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References from A Pol III-independent sgRNA expression strategy enables plasmid-compatible CRISPR/Cas9 genome editing in the Pacific oyster (Crassostrea gigas). Local targets link to admitted publications; unresolved targets remain external evidence.
Tailor-made CRISPR/Cas system for highly efficient targeted gene replacement in the rice blast fungus
10.1002/bit.25662 · 2015 · External reference
Evolutionary innovation within conserved gene regulatory networks underlying biomineralized skeletons in Bilateria
10.1093/molbev/msag019 · 2026 · External reference
Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9
10.1242/bio.20137120 · 2014 · External reference
Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level
10.1038/s41467-025-59280-6 · 2025 · External reference
Characterization and organization of the U6 snRNA gene in zebrafish and usage of their promoters to express short hairpin RNA
10.1016/j.margen.2008.10.001 · 2008 · External reference
Multiplexed and tunable transcriptional activation by promoter insertion using nuclease-assisted vector integration
10.1093/nar/gkz210 · 2019 · External reference
fastp: an ultra-fast all-in-one FASTQ preprocessor
10.1093/bioinformatics/bty560 · 2018 · External reference
Functional characterization of novel U6 RNA polymerase III promoters: their implication for CRISPR-Cas9-mediated gene editing in Aspergillus oryzae
10.1007/s00284-019-01770-0 · 2019 · External reference
Inference of CRISPR edits from sanger trace data
10.1089/crispr.2021.0113 · 2022 · External reference
Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination
10.1038/nmeth.2641 · 2013 · External reference
Multiple, dispersed human U6 small nuclear RNA genes with varied transcriptional efficiencies
10.1093/nar/gkg331 · 2003 · External reference
U6 snRNA pseudogenes: markers of retrotransposition dynamics in mammals
10.1093/molbev/msv062 · 2015 · External reference
Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo
10.1016/j.ydbio.2017.08.036 · 2017 · External reference
Genome engineering for crop improvement and future agriculture
10.1016/j.cell.2021.01.005 · 2021 · External reference
Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
10.1111/jipb.12152 · 2014 · External reference
Electroporation-mediated exogenous gene delivery methodology into germ cells of the scallop, Chlamys farreri
10.1016/j.aquaculture.2025.743514 · 2026 · External reference
Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR
10.1186/s13059-016-1012-2 · 2016 · External reference
Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants
10.1007/s11427-019-1612-6 · 2020 · External reference
Ancient repeat sequence derived from U6 snRNA in primate genomes
10.1016/j.gene.2009.07.015 · 2009 · External reference
Genetic improvement of oysters: current status, challenges, and prospects
10.1111/raq.12868 · 2023 · External reference
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector
10.1093/nar/gku749 · 2014 · External reference
Ribozyme mediated gRNA generation for in vitro and in vivo CRISPR/Cas9 mutagenesis
2016 · External reference
The sequence alignment and map format and SAMtools
10.1093/bioinformatics/btp352 · 2009 · External reference
CRISPR/Cas9 mediated high efficiency knockout of myosin essential light chain gene in the Pacific oyster (Crassostrea Gigas)
10.1007/s10126-020-10016-1 · 2021 · External reference
High resolution melting analysis: a rapid and sensitive method for detection of mutations induced by CRISPR/Cas9-based genome editing in oysters
10.1016/j.aquaculture.2023.739533 · 2023 · External reference
Dual sgRNA-directed tyrosinases knockout using CRISPR/Cas9 technology in Pacific oyster (Crassostrea gigas) reveals their roles in early shell calcification
10.1016/j.gene.2024.148748 · 2024 · External reference
Optimizing genome editing in mollusks (Crassostrea gigas) in vitro validation of sgRNA and identifying key factors influencing efficiency
10.1089/crispr.2024.0086 · 2025 · External reference
A dual-system CRISPR-LbCpf1 platform for genome editing in the bivalves (Crassostrea gigas): targeting both embryos and oocytes
10.1016/j.aquaculture.2025.743353 · 2026 · External reference
Evolution of 7SK RNA and its protein partners in Metazoa
10.1093/molbev/msp198 · 2009 · External reference
An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene
10.1093/nar/29.12.2502 · 2001 · External reference
Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells
10.1016/j.molcel.2014.04.022 · 2014 · External reference
Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters
10.1111/imb.12875 · 2023 · External reference
Efficient genome editing using CRISPR-Cas-mediated homology directed repair in the ascidian Ciona robusta
2018 · External reference
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila
10.1073/pnas.1405500111 · 2014 · External reference
Potential of genomic technologies to improve disease resistance in molluscan aquaculture
10.1098/rstb.2020.0168 · 2021 · External reference
Long-range enhancer-promoter contacts in gene expression control
10.1038/s41576-019-0128-0 · 2019 · External reference
Identification of active shrimp U6 promoters for efficient CRISPR/Cas9 gene editing in penaeid shrimps based on a VP28-pseudotyped baculovirus expression system
10.1016/j.aquaculture.2024.741128 · 2024 · External reference
Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes
10.1038/nbt.3806 · 2017 · External reference
Integrative genomics viewer (IGV): high-performance genomics data visualization and exploration
10.1093/bib/bbs017 · 2013 · External reference
Self-cleaving guide RNAs enable pharmacological selection of precise gene editing events in vivo
10.1038/s41467-022-35097-5 · 2022 · External reference
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
10.1038/nbt.3117 · 2015 · External reference
Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions
10.1093/nar/gkac582 · 2022 · External reference
Synthetic gene design—the rationale for codon optimization and implications for molecular pharming in plants
10.1002/bit.26183 · 2017 · External reference
Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system
10.1073/pnas.1420294112 · 2015 · External reference
Optimized plasmid construction strategy for Cas9
10.1159/000491669 · 2018 · External reference
Gene delivery available in molluscan cells by strong promoter discovered from bivalve-infectious virus
10.1073/pnas.2209910119 · 2022 · External reference
Targeted gene disruption in Pacific oyster based on CRISPR/Cas9 ribonucleoprotein complexes
10.1007/s10126-019-09885-y · 2019 · External reference
Comparative analysis of promoter activity in Crassostrea gigas embryos: implications for bivalve gene editing
10.1007/s10126-024-10398-6 · 2025 · External reference
The endogenous EF-1α promoter is highly active in driving gene overexpression in developing embryos of the Pacific oyster Crassostrea gigas
10.1016/j.aquaculture.2020.735134 · 2020 · External reference
Using Staphylococcus aureus Cas9 to expand the scope of potential gene targets for genome editing in Soybean
2022 · External reference
Tailor-made CRISPR/Cas system for highly efficient targeted gene replacement in the rice blast fungus
10.1002/bit.25662 · ExternalCitation · doi-reference
Synthetic gene design—the rationale for codon optimization and implications for molecular pharming in plants
10.1002/bit.26183 · ExternalCitation · doi-reference
Functional characterization of novel U6 RNA polymerase III promoters: their implication for CRISPR-Cas9-mediated gene editing in Aspergillus oryzae
10.1007/s00284-019-01770-0 · ExternalCitation · doi-reference
Targeted gene disruption in Pacific oyster based on CRISPR/Cas9 ribonucleoprotein complexes
10.1007/s10126-019-09885-y · ExternalCitation · doi-reference
CRISPR/Cas9 mediated high efficiency knockout of myosin essential light chain gene in the Pacific oyster (Crassostrea Gigas)
10.1007/s10126-020-10016-1 · ExternalCitation · doi-reference
Comparative analysis of promoter activity in Crassostrea gigas embryos: implications for bivalve gene editing
10.1007/s10126-024-10398-6 · ExternalCitation · doi-reference
Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants
10.1007/s11427-019-1612-6 · ExternalCitation · doi-reference
The endogenous EF-1α promoter is highly active in driving gene overexpression in developing embryos of the Pacific oyster Crassostrea gigas
10.1016/j.aquaculture.2020.735134 · ExternalCitation · doi-reference
High resolution melting analysis: a rapid and sensitive method for detection of mutations induced by CRISPR/Cas9-based genome editing in oysters
10.1016/j.aquaculture.2023.739533 · ExternalCitation · doi-reference
Identification of active shrimp U6 promoters for efficient CRISPR/Cas9 gene editing in penaeid shrimps based on a VP28-pseudotyped baculovirus expression system
10.1016/j.aquaculture.2024.741128 · ExternalCitation · doi-reference
A dual-system CRISPR-LbCpf1 platform for genome editing in the bivalves (Crassostrea gigas): targeting both embryos and oocytes
10.1016/j.aquaculture.2025.743353 · ExternalCitation · doi-reference
Electroporation-mediated exogenous gene delivery methodology into germ cells of the scallop, Chlamys farreri
10.1016/j.aquaculture.2025.743514 · ExternalCitation · doi-reference
Genome engineering for crop improvement and future agriculture
10.1016/j.cell.2021.01.005 · ExternalCitation · doi-reference
Ancient repeat sequence derived from U6 snRNA in primate genomes
10.1016/j.gene.2009.07.015 · ExternalCitation · doi-reference
Dual sgRNA-directed tyrosinases knockout using CRISPR/Cas9 technology in Pacific oyster (Crassostrea gigas) reveals their roles in early shell calcification
10.1016/j.gene.2024.148748 · ExternalCitation · doi-reference
Characterization and organization of the U6 snRNA gene in zebrafish and usage of their promoters to express short hairpin RNA
10.1016/j.margen.2008.10.001 · ExternalCitation · doi-reference
Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells
10.1016/j.molcel.2014.04.022 · ExternalCitation · doi-reference
Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo
10.1016/j.ydbio.2017.08.036 · ExternalCitation · doi-reference
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
10.1038/nbt.3117 · ExternalCitation · doi-reference
Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes
10.1038/nbt.3806 · ExternalCitation · doi-reference
Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination
10.1038/nmeth.2641 · ExternalCitation · doi-reference
Self-cleaving guide RNAs enable pharmacological selection of precise gene editing events in vivo
10.1038/s41467-022-35097-5 · ExternalCitation · doi-reference
Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level
10.1038/s41467-025-59280-6 · ExternalCitation · doi-reference
Long-range enhancer-promoter contacts in gene expression control
10.1038/s41576-019-0128-0 · ExternalCitation · doi-reference
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila
10.1073/pnas.1405500111 · ExternalCitation · doi-reference
Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system
10.1073/pnas.1420294112 · ExternalCitation · doi-reference
Gene delivery available in molluscan cells by strong promoter discovered from bivalve-infectious virus
10.1073/pnas.2209910119 · ExternalCitation · doi-reference
Inference of CRISPR edits from sanger trace data
10.1089/crispr.2021.0113 · ExternalCitation · doi-reference
Optimizing genome editing in mollusks (Crassostrea gigas) in vitro validation of sgRNA and identifying key factors influencing efficiency
10.1089/crispr.2024.0086 · ExternalCitation · doi-reference
Integrative genomics viewer (IGV): high-performance genomics data visualization and exploration
10.1093/bib/bbs017 · ExternalCitation · doi-reference
The sequence alignment and map format and SAMtools
10.1093/bioinformatics/btp352 · ExternalCitation · doi-reference
fastp: an ultra-fast all-in-one FASTQ preprocessor
10.1093/bioinformatics/bty560 · ExternalCitation · doi-reference
Evolutionary innovation within conserved gene regulatory networks underlying biomineralized skeletons in Bilateria
10.1093/molbev/msag019 · ExternalCitation · doi-reference
Evolution of 7SK RNA and its protein partners in Metazoa
10.1093/molbev/msp198 · ExternalCitation · doi-reference
U6 snRNA pseudogenes: markers of retrotransposition dynamics in mammals
10.1093/molbev/msv062 · ExternalCitation · doi-reference
An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene
10.1093/nar/29.12.2502 · ExternalCitation · doi-reference
Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions
10.1093/nar/gkac582 · ExternalCitation · doi-reference
Multiple, dispersed human U6 small nuclear RNA genes with varied transcriptional efficiencies
10.1093/nar/gkg331 · ExternalCitation · doi-reference
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector
10.1093/nar/gku749 · ExternalCitation · doi-reference
Multiplexed and tunable transcriptional activation by promoter insertion using nuclease-assisted vector integration
10.1093/nar/gkz210 · ExternalCitation · doi-reference
Potential of genomic technologies to improve disease resistance in molluscan aquaculture
10.1098/rstb.2020.0168 · ExternalCitation · doi-reference
Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters
10.1111/imb.12875 · ExternalCitation · doi-reference
Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
10.1111/jipb.12152 · ExternalCitation · doi-reference
Genetic improvement of oysters: current status, challenges, and prospects
10.1111/raq.12868 · ExternalCitation · doi-reference
Optimized plasmid construction strategy for Cas9
10.1159/000491669 · ExternalCitation · doi-reference
Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR
10.1186/s13059-016-1012-2 · ExternalCitation · doi-reference
Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9
10.1242/bio.20137120 · ExternalCitation · doi-reference
