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References from Extending the Lifespan of Human Mesenchymal Stromal Cells: Approaches to Immortalization and Clinical Prospects. Local targets link to admitted publications; unresolved targets remain external evidence.
Mesenchymal stem cells and their microenvironment
10.1186/s13287-022-02985-y · 2022 · External reference
The safety of MSC therapy over the past 15 years: a meta-analysis
10.1186/s13287-021-02609-x · 2021 · External reference
Challenges and advances in clinical applications of mesenchymal stromal cells
10.1186/s13045-021-01037-x · 2021 · External reference
Challenges in Clinical Development of Mesenchymal Stromal/Stem Cells: Concise Review
10.1002/sctm.19-0044 · 2019 · External reference
Inter- and Intra-donor variability in bone marrow-derived mesenchymal stromal cells: implications for clinical applications
10.1016/j.jcyt.2024.03.486 · 2024 · External reference
The potency of mesenchymal stem/stromal cells: does donor sex matter?
10.1186/s13287-024-03722-3 · 2024 · External reference
Managing the Heterogeneity of Mesenchymal Stem Cells for Cartilage Regenerative Therapy: A Review
10.3390/bioengineering10030355 · 2023 · External reference
The issue of heterogeneity of MSC-based advanced therapy medicinal products–a review
10.3389/fcell.2024.1400347 · 2024 · External reference
Advantages of pooling of human bone marrow-derived mesenchymal stromal cells from different donors versus single-donor MSCs
10.1038/s41598-024-62544-8 · 2024 · External reference
Pooled human bone marrow-derived mesenchymal stromal cells with defined trophic factors cargo promote dermal wound healing in diabetic rats by improved vascularization and dynamic recruitment of M2-like macrophages
10.3389/fimmu.2022.976511 · 2022 · External reference
Concise Review: Review and Perspective of Cell Dosage and Routes of Administration From Preclinical and Clinical Studies of Stem Cell Therapy for Heart Disease
10.5966/sctm.2015-0101 · 2016 · External reference
Recent advances in conditional cell immortalization technology, Cell Gene Ther
2016 · External reference
Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features
10.1155/2020/5726947 · 2020 · External reference
Adipose-Derived Mesenchymal Stem Cell (MSC) Immortalization by Modulation of hTERT and TP53 Expression Levels
10.3390/jpm13111621 · 2023 · External reference
From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy
10.3390/ijms222111439 · 2021 · External reference
Immune modulatory mesenchymal stem cells derived from human embryonic stem cells through a trophoblast-like stage
10.1002/stem.2242 · 2016 · External reference
Production, safety and efficacy of iPSC-derived mesenchymal stromal cells in acute steroid-resistant graft versus host disease: a phase I, multicenter, open-label, dose-escalation study
10.1038/s41591-020-1050-x · 2020 · External reference
MSC Manufacturing for Academic Clinical Trials: From a Clinical-Grade to a Full GMP-Compliant Process
10.3390/cells10061320 · 2021 · External reference
Clinical-scale expansion of mesenchymal stromal cells: a large banking experience
10.1186/s12967-016-0892-y · 2016 · External reference
Novel Immortalized Human Multipotent Mesenchymal Stromal Cell Line for Studying Hormonal Signaling
10.3390/ijms25042421 · 2024 · External reference
Immortalized human hair follicle-derived mesenchymal-like stromal cells for the long-term production of scalable Immunomodulatory and regenerative secretome
10.1186/s13287-025-04775-8 · 2025 · External reference
The PRISMA 2020 statement: an updated guideline for reporting systematic reviews
2021 · External reference
Challenges and limitations of mesenchymal stem cell therapy for lung diseases in clinical trials
10.1080/14728214.2025.2489700 · 2025 · External reference
Allogeneic vs. autologous mesenchymal stem/stromal cells in their medication practice
10.1186/s13578-021-00698-y · 2021 · External reference
Multiomics global landscape of stemness-related gene clusters in adipose-derived mesenchymal stem cells
10.1186/s13287-020-01823-3 · 2020 · External reference
Effects of long-term culture on the biological characteristics and RNA profiles of human bone-marrow-derived mesenchymal stem cells
10.1016/j.omtn.2021.08.013 · 2021 · External reference
Concise Review: Mesenchymal Stem Cells Derived from Human Pluripotent Cells, an Unlimited and Quality-Controllable Source for Therapeutic Applications
10.1002/stem.2964 · 2019 · External reference
Human embryonic stem cell–derived mesenchymal stromal cells suppress inflammation in mouse models of rheumatoid arthritis and lung fibrosis by regulating T-cell function
10.1016/j.jcyt.2024.03.008 · 2024 · External reference
Induced pluripotent stem cell-derived mesenchymal stem cells: whether they can become new stars of cell therapy
10.1186/s13287-024-03968-x · 2024 · External reference
Progress and emerging techniques for biomaterial-based derivation of mesenchymal stem cells (MSCs) from pluripotent stem cells (PSCs)
10.1186/s40824-023-00371-0 · 2023 · External reference
Low-Intensity Pulsed Ultrasound Promotes Osteogenic Potential of iPSC-Derived MSCs but Fails to Simplify the iPSC-EB-MSC Differentiation Process
10.3389/fbioe.2022.841778 · 2022 · External reference
Human iPSC-derived iMSCs improve bone regeneration in mini-pigs
10.1038/s41413-019-0069-4 · 2019 · External reference
Feeder-free generation and transcriptome characterization of functional mesenchymal stromal cells from human pluripotent stem cells
10.1016/j.scr.2020.101990 · 2020 · External reference
In vivo regeneration of rat laryngeal cartilage with mesenchymal stem cells derived from human induced pluripotent stem cells via neural crest cells
10.1016/j.scr.2021.102233 · 2021 · External reference
Human induced mesenchymal stem cells display increased sensitivity to matrix stiffness
10.1038/s41598-022-12143-2 · 2022 · External reference
Systemic Supplementation of Collagen VI by Neonatal Transplantation of iPSC-Derived MSCs Improves Histological Phenotype and Function of Col6-Deficient Model Mice
10.3389/fcell.2021.790341 · 2021 · External reference
Laryngeal Cartilage Regeneration of Nude Rats by Transplantation of Mesenchymal Stem Cells Derived from Human-Induced Pluripotent Stem Cells
10.1177/09636897231178460 · 2023 · External reference
Impact of differentiation protocols on the functionality of mesenchymal stem cells derived from induced pluripotent stem cells
10.4252/wjsc.v17.i12.110564 · 2025 · External reference
Mesenchymal Stem Cells (MSC) Derived from Induced Pluripotent Stem Cells (iPSC) Equivalent to Adipose-Derived MSC in Promoting Intestinal Healing and Microbiome Normalization in Mouse Inflammatory Bowel Disease Model
10.1002/sctm.17-0305 · 2018 · External reference
The therapeutic effects of induced pluripotent stem cell‐derived mesenchymal stem cells on Parkinson’s disease
10.1002/iub.2936 · 2025 · External reference
Induced pluripotent stem cell‐derived mesenchymal stem cells prolong hind limb survival in a rat vascularized composite allotransplantation model
10.1002/micr.30507 · 2019 · External reference
Generation of canine induced pluripotent stem cell-derived mesenchymal stem cells: Comparison of differentiation strategies and cell origins
10.1016/j.reth.2025.05.008 · 2025 · External reference
iPSC-derived mesenchymal stem cells attenuate cerebral ischemia-reperfusion injury by inhibiting inflammatory signaling and oxidative stress
10.1016/j.omtm.2023.07.005 · 2023 · External reference
Kartogenin Enhances Chondrogenic Differentiation of iPSC Derived MSCs (iMSCs) and Improves Outcomes in an Osteochondral Defect Model in Male Rats
10.1002/jor.26040 · 2025 · External reference
Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients
10.3389/fimmu.2023.1022676 · 2023 · External reference
Differentiation and Molecular Properties of Mesenchymal Stem Cells Derived from Murine Induced Pluripotent Stem Cells Derived on Gelatin or Collagen
10.1155/2016/9013089 · 2016 · External reference
Safety and immune regulatory properties of canine induced pluripotent stem cell-derived mesenchymal stem cells
10.1016/j.scr.2017.11.010 · 2017 · External reference
Potential and Limitations of Induced Pluripotent Stem Cells-Derived Mesenchymal Stem Cells in Musculoskeletal Disorders Treatment
10.3390/biom13091342 · 2023 · External reference
CymerusTM iPSC-MSCs significantly prolong survival in a pre-clinical, humanized mouse model of Graft-vs-host disease
10.1016/j.scr.2019.101401 · 2019 · External reference
Induced Pluripotent Stem Cell-Derived Mesenchymal Stromal Cells Are Functionally and Genetically Different From Bone Marrow-Derived Mesenchymal Stromal Cells
10.1002/stem.2993 · 2019 · External reference
Methods to produce induced pluripotent stem cell-derived mesenchymal stem cells: Mesenchymal stem cells from induced pluripotent stem cells, World J
2021 · External reference
Inducible Caspase9-mediated suicide gene for MSC-based cancer gene therapy
10.1038/s41417-018-0034-1 · 2019 · External reference
Regulatory considerations for developing a phase I investigational new drug application for autologous induced pluripotent stem cells‐based therapy product
10.1002/sctm.20-0242 · 2020 · External reference
Application of telomere biology and telomerase in mesenchymal stem cells
10.26599/ntm.2022.9130007 · 2022 · External reference
Immortalization of Mesenchymal Stem Cells for Application in Regenerative Medicine and Their Potential Risks of Tumorigenesis
10.3390/ijms252413562 · 2024 · External reference
Transformed Canine and Murine Mesenchymal Stem Cells as a Model for Sarcoma with Complex Genomics
10.3390/cancers13051126 · 2021 · External reference
Revisiting the role of mesenchymal stromal cells in cancer initiation, metastasis and immunosuppression
10.1186/s40164-024-00532-4 · 2024 · External reference
Alterations of epigenetic regulators and P53 mutations in murine mesenchymal stem cell cultures: A possible mechanism of spontaneous transformation
10.3233/cbm-203121 · 2021 · External reference
Phase separation of SHP2E76K promotes malignant transformation of mesenchymal stem cells by activating mitochondrial complexes
10.1172/jci.insight.170340 · 2024 · External reference
Neoplastic Transformation of Human Mesenchymal Stromal Cells Mediated via LIN28B
10.1038/s41598-019-44536-1 · 2019 · External reference
Mechanisms of human telomerase reverse transcriptase (hTERT) regulation: clinical impacts in cancer
10.1186/s12929-018-0422-8 · 2018 · External reference
Human mesenchymal stem cells with enhanced telomerase activity acquire resistance against oxidative stress-induced genomic damage
10.1016/j.jcyt.2017.03.078 · 2017 · External reference
Establishing an hTERT-driven immortalized umbilical cord-derived mesenchymal stem cell line and its therapeutic application in mice with liver failure
10.1177/20417314231200328 · 2023 · External reference
Development of a Biologically Immortalized Equine Stem Cell Line
10.1055/s-0038-1668241 · 2018 · External reference
Characterization of human telomerase reverse transcriptase immortalized anterior cruciate ligament cell lines
10.1016/j.bj.2019.05.005 · 2019 · External reference
10.1007/s12015-020-09986-5 · 2020 · Admitted local publication
Decreased Insulin Sensitivity in Telomerase-Immortalized Mesenchymal Stem Cells Affects Efficacy and Outcome of Adipogenic Differentiation in vitro
10.3389/fcell.2021.662078 · 2021 · External reference
hTERT-immortalized adipose-derived stem cell line ASC52Telo demonstrates limited potential for adipose biology research
10.1016/j.ab.2021.114268 · 2021 · External reference
The other side of the coin: mesenchymal stromal cell immortalization beyond evasion of senescence
10.1007/s13577-023-00925-3 · 2023 · External reference
Immortalized canine adipose-derived mesenchymal stem cells alleviate gentamicin-induced acute kidney injury by inhibiting endoplasmic reticulum stress in mice and dogs
10.1016/j.rvsc.2021.02.001 · 2021 · External reference
Characterization of canine adipose tissue‐derived mesenchymal stem cells immortalized by SV40‐T retrovirus for therapeutic use
10.1002/jcp.28338 · 2019 · External reference
CRISPR/Cas9-mediated reversibly immortalized mouse bone marrow stromal stem cells (BMSCs) retain multipotent features of mesenchymal stem cells (MSCs)
10.18632/oncotarget.22915 · 2017 · External reference
Immortalized Mesenchymal Stem Cells: A Safe Cell Source for Cellular or Cell Membrane-Based Treatment of Glioma
2022 · External reference
Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for Obtention
10.3390/biotech12010014 · 2023 · External reference
Reversibly immortalized human umbilical cord–derived mesenchymal stem cells (UC‐MSCs) are responsive to BMP9‐induced osteogenic and adipogenic differentiation
10.1002/jcb.27140 · 2018 · External reference
Rejuvenation of human mesenchymal stem cells using a nonintegrative and conditionally removable Sendai virus vector
10.1038/s41598-024-74757-y · 2024 · External reference
Immortalization Reversibility in the Context of Cell Therapy Biosafety
10.3390/ijms24097738 · 2023 · External reference
Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels
10.1038/srep27697 · 2016 · External reference
Meeting FDA Guidance recommendations for replication-competent virus and insertional oncogenesis testing
10.1016/j.omtm.2022.11.009 · 2023 · External reference
Development of the First World Health Organization Lentiviral Vector Standard: Toward the Production Control and Standardization of Lentivirus-Based Gene Therapy Products
10.1089/hgtb.2017.078 · 2017 · External reference
Immortalized Canine Adipose-Derived Mesenchymal Stem Cells as a Novel Candidate Cell Source for Mesenchymal Stem Cell Therapy
10.3390/ijms24032250 · 2023 · External reference
Novel immortalization approach defers senescence of cultured canine adipose-derived mesenchymal stromal cells
10.1007/s11357-021-00488-x · 2022 · External reference
Characterization of a growth-elevated cell line of human bone marrow-derived mesenchymal stem cells by SV40 T-antigen
10.1007/s12257-014-0730-0 · 2015 · External reference
Immortalization of canine adipose‐derived mesenchymal stem cells and their seminiferous tubule transplantation
10.1002/jcb.26574 · 2018 · External reference
Epigenetic regulation of mesenchymal stem cell aging through histone modifications
10.1016/j.gendis.2022.10.030 · 2022 · External reference
Mesenchymal stem cells under epigenetic control – the role of epigenetic machinery in fate decision and functional properties
10.1038/s41419-023-06239-4 · 2023 · External reference
Tracing genomic instability in induced mesenchymal stromal cell manufacture: an integration-free transfection approach
10.1038/s12276-025-01439-8 · 2025 · External reference
Single-cell Transcriptomic Analysis Reveals the Cellular Heterogeneity of Mesenchymal Stem Cells
10.1016/j.gpb.2022.01.005 · 2022 · External reference
Practical Use of Immortalized Cells in Medicine: Current Advances and Future Perspectives
10.3390/ijms241612716 · 2023 · External reference
Engineered Stem Cells Improve Neurogenic Bladder by Overexpressing SDF-1 in a Pelvic Nerve Injury Rat Model
10.1177/0963689720902466 · 2020 · External reference
Human iPSC-Derived MSCs Induce Neurotrophic Effects and Improve Metabolic Activity in Acute Neuronal Injury Models
10.1523/jneurosci.0606-24.2024 · 2025 · External reference
BDNF-Overexpressing Engineered Mesenchymal Stem Cells Enhances Their Therapeutic Efficacy against Severe Neonatal Hypoxic Ischemic Brain Injury
10.3390/ijms222111395 · 2021 · External reference
Immortalized Human Bone Marrow Derived Stromal Cells in Treatment of Transient Cerebral Ischemia in Rats
10.3233/jad-190279 · 2019 · External reference
Mesenchymal stem cells overexpressing BMP-9 by CRISPR-Cas9 present high in vitro osteogenic potential and enhance in vivo bone formation
10.1038/s41434-021-00248-8 · 2021 · External reference
Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability
10.1002/ame2.12513 · 2024 · External reference
Bone morphogenetic protein 9 (BMP9) induces effective bone formation from reversibly immortalized multipotent adipose-derived (iMAD) mesenchymal stem cells
2016 · External reference
Fluorescent Immortalized Human Adipose Derived Stromal Cells (hASCs-TS/GFP+) for Studying Cell Drug Delivery Mediated by Microvesicles
10.2174/1871520617666170327113932 · 2017 · External reference
Biomimetic immortalized mesenchymal stem cell-based nanoparticles suppress orthotopic postsurgical glioma via CD73 targeting and chemotherapy
10.1016/j.jconrel.2025.114297 · 2025 · External reference
Local injection of CCL19-expressing mesenchymal stem cells augments the therapeutic efficacy of anti-PD-L1 antibody by promoting infiltration of immune cells
10.1136/jitc-2020-000582 · 2020 · External reference
Immortalized mesenchymal stromal cells overexpressing alpha‐1 antitrypsin protect acinar cells from apoptotic and ferroptotic cell death
10.1111/jcmm.70093 · 2024 · External reference
Bioluminescence Imaging of Transplanted Mesenchymal Stem Cells by Overexpression of Hepatocyte Nuclear Factor4α: Tracking Biodistribution and Survival
10.1007/s11307-018-1204-0 · 2019 · External reference
Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats
10.1590/1414-431x2021e11352 · 2021 · External reference
Generation of Functional Immortalized Human Corneal Stromal Stem Cells
10.3390/ijms232113399 · 2022 · External reference
Evaluating teratoma formation risk of pluripotent stem cell-derived cell therapy products: a consensus recommendation from the Health and Environmental Sciences Institute’s International Cell Therapy Committee
10.1016/j.jcyt.2025.04.062 · 2025 · External reference
Flexible and Expedited Regulatory Review Processes for Innovative Medicines and Regenerative Medical Products in the US, the EU, and Japan
10.3390/ijms20153801 · 2019 · External reference
Genetic Stability of Mesenchymal Stromal Cells for Regenerative Medicine Applications: A Fundamental Biosafety Aspect
10.3390/ijms20102406 · 2019 · External reference
10.1007/978-981-16-0301-3_5
10.1007/978-981-16-0301-3_5 · External reference
Clonal chromosomal and genomic instability during human multipotent mesenchymal stromal cells long-term culture
10.1371/journal.pone.0192445 · 2018 · External reference
Establishment of Three Types of Immortalized Human Skin Stem Cell Lines Derived from the Single Donor
10.1248/bpb.b21-00058 · 2021 · External reference
Low-pass whole genome sequencing as a cost-effective alternative to chromosomal microarray analysis for low- and middle-income countries
10.1002/ajmg.a.63802 · 2024 · External reference
An NGS-based approach for precise and footprint-free CRISPR-based gene editing in human stem cells
10.1016/j.ymeth.2025.05.004 · 2025 · External reference
Optical Genome Mapping: A New Tool for Cytogenomic Analysis
10.3390/genes16080924 · 2025 · External reference
Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs
10.1186/s13059-020-02143-8 · 2020 · External reference
Microvesicles from Human Immortalized Cell Lines of Endothelial Progenitor Cells and Mesenchymal Stem/Stromal Cells of Adipose Tissue Origin as Carriers of Bioactive Factors Facilitating Angiogenesis
10.1155/2020/1289380 · 2020 · External reference
Exosomes derived from mesenchymal stem cells primed with disease-condition-serum improved therapeutic efficacy in a mouse rheumatoid arthritis model via enhanced TGF-β1 production
10.1186/s13287-023-03523-0 · 2023 · External reference
Practical Considerations for Translating Mesenchymal Stromal Cell-Derived Extracellular Vesicles from Bench to Bed
10.3390/pharmaceutics14081684 · 2022 · External reference
Applications, challenges and prospects of mesenchymal stem cell exosomes in regenerative medicine
10.1186/s13287-021-02596-z · 2021 · External reference
Current challenges surrounding exosome treatments
2023 · External reference
hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells
10.3390/cells13100861 · 2024 · External reference
Mesenchymal Stem Cells Current Clinical Applications: A Systematic Review
10.1016/j.arcmed.2020.08.006 · 2021 · External reference