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References from A hierarchical systems metabolic engineering framework for modular construction of Mycolicibacterium neoaurumcell factories for efficient phytosterol bioconversion. Local targets link to admitted publications; unresolved targets remain external evidence.
Unresolved reference
2018 · External reference
Mycobacterial cytochrome P450 125 (Cyp125) catalyzes the terminal hydroxylation of C27 steroids
10.1074/jbc.m109.072132 · 2009 · External reference
Structure of an endogenous mycobacterial MCE lipid transporter
10.1038/s41586-023-06366-0 · 2023 · External reference
Steroid bioconversions. methods
2017 · External reference
Engineering a two-enzyme system in Mycolicibacterium neoaurum for efficient biotransformation of phytosterols to dihydrotestosterone
10.1016/j.ijbiomac.2024.138443 · 2025 · External reference
Bioconversion of phytosterols to 9-Hydroxy-3-Oxo-4,17-Pregadiene-20-Carboxylic Acid Methyl Ester by Enoyl-CoA deficiency and modifying multiple genes in Mycolicibacterium neoaurum
10.1128/aem.01303-22 · 2022 · External reference
Microbial steroid transformations: current state and prospects
10.1007/s00253-012-4078-0 · 2012 · External reference
Engineering Mycobacterium smegmatis for testosterone production
10.1111/1751-7915.12433 · 2017 · External reference
New insights on steroid biotechnology
10.3389/fmicb.2018.00958 · 2018 · External reference
Mycobacterium smegmatis is a suitable cell factory for the production of steroidic synthons
10.1111/1751-7915.12429 · 2017 · External reference
Catabolism and biotechnological applications of cholesterol degrading bacteria
10.1111/j.1751-7915.2012.00331.x · 2012 · External reference
Efficient production of 9,22-Dihydroxy-23,24-bisnorchol-4-ene-3-one from Phytosterols by Modifying Multiple Genes in Mycobacterium fortuitum
10.3390/ijms25073579 · 2024 · External reference
Engineering phytosterol transport system in Mycobacterium sp. strain MS136 enhances production of 9α-hydroxy-4-androstene-3,17-dione
10.1007/s10529-018-2520-9 · 2018 · External reference
Highly accurate protein structure prediction with AlphaFold
10.1038/s41586-021-03819-2 · 2021 · External reference
Rerouting phytosterol degradation pathway for directed androst-1,4-diene-3,17-dione microbial bioconversion
10.1007/s00253-023-12847-z · 2024 · External reference
Structures and molecular mechanisms of action of the cholesterol C17 side-chain-degrading enzymes
2022 · External reference
Microbial catabolism of sterols: focus on the enzymes that transform the sterol 3β-hydroxy-5-en into 3-keto-4-en
2017 · External reference
Introduction
2010 · External reference
Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9alpha-hydroxylated derivatives by engineered Mycobacteria
10.1186/s12934-021-01653-9 · 2021 · External reference
Regulation of NAD(H) pool and NADH/NAD(+) ratio by overexpression of nicotinic acid phosphoribosyltransferase for succinic acid production in Escherichia coli NZN111
10.1016/j.enzmictec.2012.07.011 · 2012 · External reference
Emerging gene editing in industrial microbiology beyond CRISPR-Cas9
10.1016/j.tibtech.2024.09.012 · 2025 · External reference
Engineered 3-Ketosteroid 9α-Hydroxylases in Mycobacterium neoaurum: an efficient platform for production of steroid drugs
10.1128/aem.02777-17 · 2018 · External reference
CRISPR-Cas12a assisted precise genome editing of Mycolicibacterium neoaurum
10.1016/j.nbt.2021.10.003 · 2022 · External reference
Metabolic adaptation of Mycobacterium neoaurum ATCC 25795 in the catabolism of sterols for producing important steroid intermediates
10.1021/acs.jafc.8b04777 · 2018 · External reference
Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis
2022 · External reference
Improving the production of 9alpha-hydroxy-4-androstene-3,17-dione from phytosterols by 3-ketosteroid-Delta(1)-dehydrogenase deletions and multiple genetic modifications in Mycobacterium fortuitum
10.1186/s12934-023-02052-y · 2023 · External reference
The actinobacterial mce4 locus encodes a steroid transporter*
10.1074/jbc.m805496200 · 2008 · External reference
Microbial mutagenesis by atmospheric and room-temperature plasma (ARTP): the latest development
10.1186/s40643-018-0200-1 · 2018 · External reference
An orphaned Mce-associated membrane protein of Mycobacterium tuberculosis is a virulence factor that stabilizes Mce transporters
10.1111/mmi.13303 · 2016 · External reference
Evidence for the mycobacterial Mce4 transporter being a Multiprotein complex
10.1128/jb.00685-20 · 2021 · External reference
Application of microbial 3-ketosteroid Delta(1)-dehydrogenases in biotechnology
10.1016/j.biotechadv.2021.107751 · 2021 · External reference
FadA5 a thiolase from Mycobacterium tuberculosis: a steroid-binding pocket reveals the potential for drug development against tuberculosis
10.1016/j.str.2014.10.010 · 2015 · External reference
Cofactor engineering to regulate NAD(+)/NADH ratio with its application to phytosterols biotransformation
10.1186/s12934-017-0796-4 · 2017 · External reference
Whole-genome and enzymatic analyses of an androstenedione-producing Mycobacterium strain with residual phytosterol-degrading pathways
10.1186/s12934-020-01442-w · 2020 · External reference
A new steroid-transforming strain of Mycobacterium neoaurum and cloning of 3-ketosteroid 9alpha-hydroxylase in NwIB-01
10.1007/s12010-010-8919-y · 2010 · External reference
The role of fadD19 and echA19 in sterol side chain degradation by Mycobacterium smegmatis
10.3390/molecules21050598 · 2016 · External reference
Enhancement of 9α-Hydroxy-4-androstene-3,17-dione production from soybean phytosterols by deficiency of a regulated intramembrane proteolysis metalloprotease in Mycobacterium neoaurum
10.1021/acs.jafc.7b03766 · 2017 · External reference
Enhancing the bioconversion of phytosterols to steroidal intermediates by the deficiency of kasB in the cell wall synthesis of Mycobacterium neoaurum
10.1186/s12934-020-01335-y · 2020 · External reference
Unraveling and engineering the production of 23,24-bisnorcholenic steroids in sterol metabolism
10.1038/srep21928 · 2016 · External reference
CRISPR-Cas12a-assisted recombineering in bacteria
10.1128/aem.00947-17 · 2017 · External reference
Characterization and engineering of 3-ketosteroid- big up tri, open1-dehydrogenase and 3-ketosteroid-9alpha-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9alpha-hydroxy-4-androstene-3,17-dione through the catabolism of sterols
10.1016/j.ymben.2014.05.005 · 2014 · External reference
Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool
10.1007/s00253-014-5755-y · 2014 · External reference
New insights into the modification of the non-core metabolic pathway of steroids in Mycolicibacterium and the application of fermentation biotechnology in C-19 steroid production
10.3390/ijms24065236 · 2023 · External reference
Mycolicibacterium cell factory for the production of steroid-based drug intermediates
10.1016/j.biotechadv.2021.107860 · 2021 · External reference
Efficient production of androstenedione by repeated batch fermentation in waste cooking oil media through regulating NAD(+)/NADH ratio and strengthening cell vitality of Mycobacterium neoaurum
10.1016/j.biortech.2019.01.144 · 2019 · External reference
