Research graph
References from Sustainable biomanufacturing of paclitaxel: From pathway elucidation to green production systems. Local targets link to admitted publications; unresolved targets remain external evidence.
Metabolic engineering of Bacillus subtilis toward taxadiene biosynthesis as the first committed step for taxol production
10.3389/fmicb.2019.00218 · 2019 · External reference
Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli
10.1126/science.1191652 · 2010 · External reference
An endoplasmic reticulum-engineered yeast platform for overproduction of triterpenoids
10.1016/j.ymben.2017.02.007 · 2017 · External reference
Engineering of Yarrowia lipolytica for terpenoid production
10.1016/j.mec.2022.e00213 · 2022 · External reference
Accessing low-oxidation state taxanes: is taxadiene-4(5)-epoxide on the taxol biosynthetic pathway?
10.1039/c5sc03463a · 2016 · External reference
Rational engineering of photosynthetic electron flux enhances light-powered cytochrome P450 activity
2018 · External reference
Overcoming heterologous protein interdependency to optimize P450-mediated Taxol precursor synthesis in Escherichia coli
10.1073/pnas.1515826113 · 2016 · External reference
Engineering yeast for the production of plant terpenoids using synthetic biology approaches
10.1039/d3np00005b · 2023 · External reference
Molecular cloning and characterization of a cytochrome P450 taxoid 2alpha-hydroxylase involved in Taxol biosynthesis
10.1016/j.abb.2004.04.016 · 2004 · External reference
Taxol biosynthesis: molecular cloning and characterization of a cytochrome P450 taxoid 7-hydroxylase
2004 · External reference
Regioselectivity of taxoid-O-acetyltransferases: heterologous expression and characterization of a new taxadien-5alpha-ol-O-acetyltransferase
10.1016/j.abb.2004.07.013 · 2004 · External reference
Genetic engineering of taxol biosynthetic genes in Saccharomyces cerevisiae
10.1002/bit.20694 · 2006 · External reference
Genomic and metabolic instability during long-term fermentation of an industrial Saccharomyces cerevisiae strain engineered for C5 sugar utilization
10.3389/fbioe.2024.1357671 · 2024 · External reference
Metabolic engineering of taxadiene biosynthesis in yeast as a first step towards Taxol (Paclitaxel) production
10.1016/j.ymben.2008.03.001 · 2008 · External reference
A novel step towards the heterologous biosynthesis of paclitaxel: characterization of T1βOH taxane hydroxylase
10.1016/j.ymben.2024.08.005 · 2024 · External reference
Post-genomic illumination of paclitaxel biosynthesis
10.1038/s41477-024-01869-8 · 2024 · External reference
Bioprospecting and incentives for biodiversity conservation: lessons from the history of paclitaxel
2023 · External reference
Simultaneous determination of taxoids and flavonoids in twigs and leaves of three Taxus species by UHPLC-MS/MS
10.1016/j.jpba.2020.113456 · 2020 · External reference
In situ recovery of taxadiene using solid adsorption in cultivations with Saccharomyces cerevisiae
10.1080/10826068.2023.2207204 · 2024 · External reference
Production and bioprocessing of Taxol from Aspergillus niger, an endophyte of Encephalartos whitelockii, with a plausible biosynthetic stability: antiproliferative activity and cell cycle analysis
10.1186/s12934-024-02356-7 · 2024 · External reference
Research progress on Taxus extraction and formulation preparation technologies
2024 · External reference
Engineering an artificial Taxol biosynthetic pathway from baccatin III in yeast
10.1021/acssynbio.6c00148 · 2026 · External reference
Thylakoid targeting improves stability of a cytochrome P450 in the cyanobacterium Synechocystis sp. PCC 6803
10.1021/acssynbio.4c00800 · 2025 · External reference
10.1042/bsr20171290
10.1042/bsr20171290 · External reference
Cloning and functional expression of a cDNA encoding geranylgeranyl diphosphate synthase from Taxus canadensis and assessment of the role of this prenyltransferase in cells induced for taxol production
10.1006/abbi.1998.0926 · 1998 · External reference
Cytochrome P450-catalyzed hydroxylation of taxa-4(5),11(12)-diene to taxa-4(20),11(12)-dien-5alpha-ol: the first oxygenation step in taxol biosynthesis
10.1016/s1074-5521(96)90096-4 · 1996 · External reference
Efficient heterologous expression of cytochrome P450 enzymes in microorganisms for the biosynthesis of natural products
10.1080/07388551.2022.2029344 · 2023 · External reference
Asymmetric total synthesis of Taxol
10.1021/jacs.1c09637 · 2021 · External reference
Engineering Escherichia coli for the synthesis of taxadiene, a key intermediate in the biosynthesis of taxol
10.1016/s0968-0896(01)00072-4 · 2001 · External reference
Taxol biosynthesis: taxane 13 alpha-hydroxylase is a cytochrome P450-dependent monooxygenase
10.1073/pnas.251539398 · 2001 · External reference
Cytochrome P450 taxadiene 5α-hydroxylase, a mechanistically unusual monooxygenase catalyzing the first oxygenation step of Taxol biosynthesis
10.1016/j.chembiol.2004.02.022 · 2004 · External reference
Characterization and heterologous reconstitution of Taxus biosynthetic enzymes leading to baccatin III
10.1126/science.adj3484 · 2024 · External reference
Metabolic engineering of Saccharomyces cerevisiae for enhanced taxadiene production
10.1186/s12934-024-02512-z · 2024 · External reference
Heterologous expression of a bacterial haemoglobin improves the growth properties of recombinant Escherichia coli
10.1038/331633a0 · 1988 · External reference
Tailoring the Saccharomyces cerevisiae endoplasmic reticulum for functional assembly of terpene synthesis pathway
10.1016/j.ymben.2019.08.013 · 2019 · External reference
Cyclization of geranylgeranyl diphosphate to taxa-4(5),11(12)-diene is the committed step of taxol biosynthesis in Pacific yew
10.1074/jbc.270.15.8686 · 1995 · External reference
Iso-Seq analysis of the Taxus cuspidata transcriptome reveals the complexity of Taxol biosynthesis
10.1186/s12870-019-1809-8 · 2019 · External reference
Designing an oxidase toolbox for site-directed oxidation of taxanes
10.1038/s41467-025-67544-4 · 2025 · External reference
Phycobilisome-deficient strains of Synechocystis sp. PCC 6803 have reduced size and require carbon-limiting conditions to exhibit enhanced productivity
10.1104/pp.114.237206 · 2014 · External reference
Modular pathway engineering for enhancing terpenoid production in plants
10.1016/j.ymben.2026.03.010 · 2026 · External reference
Jasmonate-responsive expression of paclitaxel biosynthesis genes in Taxus cuspidata cultured cells is negatively regulated by the bHLH transcription factors TcJAMYC1, TcJAMYC2, and TcJAMYC4
10.3389/fpls.2015.00115 · 2015 · External reference
Chloroplastic metabolic engineering coupled with isoprenoid pool enhancement for committed taxanes biosynthesis in Nicotiana benthamiana
10.1038/s41467-019-12879-y · 2019 · External reference
10.1007/s44307-024-00032-w
10.1007/s44307-024-00032-w · External reference
Functional analysis of a WRKY transcription factor involved in transcriptional activation of the DBAT gene in Taxus chinensis
10.1111/j.1438-8677.2012.00611.x · 2013 · External reference
Elucidation of the final steps in Taxol biosynthesis and its biotechnological production
10.1038/s44160-025-00800-z · 2025 · External reference
Reconstitution of early paclitaxel biosynthetic network
10.1038/s41467-024-45574-8 · 2024 · External reference
Computational tools for nonnatural pathway design: algorithms, applications, and challenges
10.1016/j.bidere.2025.100041 · 2025 · External reference
Paclitaxel drug-drug interactions in the military health system
2024 · External reference
Improved production of Taxol(®) precursors in S. cerevisiae using combinatorial in silico design and metabolic engineering
10.1186/s12934-023-02251-7 · 2023 · External reference
Machine learning in enzyme engineering
2019 · External reference
Discovery of FoTO1 and Taxol genes enables biosynthesis of baccatin III
10.1038/s41586-025-09090-z · 2025 · External reference
Recent advances in metabolic engineering, protein engineering, and transcriptome-guided insights toward synthetic production of Taxol
10.3389/fbioe.2021.632269 · 2021 · External reference
Systematic study of genomic loci in Escherichia coli B and K12 for genomic integration: application in plasmid-free astaxanthin production
10.1016/j.ymben.2026.03.013 · 2026 · External reference
Exploring optimal Taxol® CYP725A4 activity in Saccharomyces cerevisiae
10.1186/s12934-022-01922-1 · 2022 · External reference
Rewiring Saccharomyces cerevisiae metabolism for optimised Taxol® precursors production
10.1016/j.mec.2023.e00229 · 2024 · External reference
Enhanced production of taxadiene in Saccharomyces cerevisiae
10.1186/s12934-020-01458-2 · 2020 · External reference
Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase
10.1111/pbi.12359 · 2016 · External reference
CYP725A4 from yew catalyzes complex structural rearrangement of taxa-4(5),11(12)-diene into the cyclic ether 5(12)-oxa-3(11)-cyclotaxane
10.1074/jbc.m708950200 · 2008 · External reference
Transcriptional knockdown of GGPPS increases cellular FPP availability and heterologous sesquiterpene production in the green microalga Chlamydomonas reinhardtii
10.1021/acssynbio.6c00033 · 2026 · External reference
Promotion of microtubule assembly in vitro by taxol
10.1038/277665a0 · 1979 · External reference
Molecular cloning of a cytochrome P450 taxane 10 beta-hydroxylase cDNA from Taxus and functional expression in yeast
10.1073/pnas.98.4.1501 · 2001 · External reference
Sites of biosynthesis and storage of Taxol in Taxus media (Rehder) plants: mechanism of accumulation
10.1016/j.phytochem.2020.112369 · 2020 · External reference
Same, but different: Variations in fragment ions among stereoisomers of a 17α-methyl steroid in gas chromatography/electron ionization mass spectrometry
10.1002/rcm.9934 · 2025 · External reference
Regulatory microRNAs and phasiRNAs of paclitaxel biosynthesis in Taxus chinensis
2024 · External reference
A synthetic biology roadmap for sustainable production of the plant-originated anti-cancer drug paclitaxel
10.1016/j.tibtech.2025.11.013 · 2026 · External reference
Advances in analysis of microbial metabolic fluxes via (13)C isotopic labeling
10.1002/mas.20191 · 2009 · External reference
Investigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis
10.1186/s12934-019-1235-5 · 2019 · External reference
Network analysis of the MVA and MEP pathways for isoprenoid synthesis
10.1146/annurev-arplant-050312-120116 · 2013 · External reference
Molecular cloning of a taxa-4(20),11(12)-dien-5alpha-ol-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli
10.1006/abbi.1999.1609 · 2000 · External reference
The final acylation step in taxol biosynthesis: cloning of the taxoid C13-side-chain N-benzoyltransferase from Taxus
10.1073/pnas.082115799 · 2002 · External reference
Molecular cloning and heterologous expression of the C-13 phenylpropanoid side chain-CoA acyltransferase that functions in Taxol biosynthesis
10.1073/pnas.192463699 · 2002 · External reference
Cloning, heterologous expression, and characterization of a phenylalanine aminomutase involved in Taxol biosynthesis
10.1074/jbc.m411215200 · 2004 · External reference
Definitive screening accelerates Taxol biosynthetic pathway optimization and scale up in Saccharomyces cerevisiae cell factories
10.1002/biot.202100414 · 2022 · External reference
Optimizing the biosynthesis of oxygenated and acetylated Taxol precursors in Saccharomyces cerevisiae using advanced bioprocessing strategies
10.1002/bit.27569 · 2021 · External reference
Acetylation–deacetylation-driven natural product biosynthesis
10.1002/ceur.202500025 · 2025 · External reference
Facile biosynthesis of taxadiene by a newly constructed Escherichia coli strain fusing enzymes taxadiene synthase and geranylgeranyl pyrophosphate synthase
10.1016/j.procbio.2022.09.005 · 2022 · External reference
Exploring the combinatorial space of complete pathways to chemicals
10.1042/bst20170272 · 2018 · External reference
The marine diatom Phaeodactylum tricornutum as a versatile bioproduction chassis: current progress, challenges, and perspectives
10.1016/j.xplc.2025.101519 · 2025 · External reference
Natural taxanes: developments since 1828
10.1021/cr100147u · 2011 · External reference
Nature as a remarkable chemist: a personal story of the discovery and development of Taxol
10.1097/cad.0000000000000063 · 2014 · External reference
Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia
10.1021/ja00738a045 · 1971 · External reference
A cDNA clone for taxadiene synthase, the diterpene cyclase that catalyzes the committed step of taxol biosynthesis
10.1074/jbc.271.16.9201 · 1996 · External reference
Biomimetic light dilution using side-emitting optical fiber for enhancing the productivity of microalgae reactors
10.1038/s41598-019-45955-w · 2019 · External reference
Construction of an Escherichia coli cell factory to synthesize taxadien-5α-ol, the key precursor of anti-cancer drug paclitaxel
10.1186/s40643-022-00569-5 · 2022 · External reference
Machine learning-assisted directed protein evolution with combinatorial libraries
10.1073/pnas.1901979116 · 2019 · External reference
Enhancing the biosynthesis of taxadien-5α-yl-acetate in Escherichia coli by combinatorial metabolic engineering approaches
10.1186/s40643-024-00762-8 · 2024 · External reference
From Taxus to paclitaxel: Opportunities and challenges for urban agriculture to promote human health
10.1016/j.plaphy.2025.109502 · 2025 · External reference
The Taxus genome provides insights into paclitaxel biosynthesis
10.1038/s41477-021-00963-5 · 2021 · External reference
Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica
10.1016/j.synbio.2023.04.002 · 2023 · External reference
Strategies to increase the robustness of microbial cell factories
10.1007/s44307-024-00018-8 · 2024 · External reference
Enhancing cross-organelle coordination to advance plant cytochrome P450 functionality in yeast
10.1126/sciadv.ady7184 · 2025 · External reference
Enhanced C30 carotenoid production in Bacillus subtilis by systematic overexpression of MEP pathway genes
10.1007/s00253-015-6531-3 · 2015 · External reference
Biosynthesis of the highly oxygenated tetracyclic core skeleton of Taxol
10.1038/s41467-024-46583-3 · 2024 · External reference
Comprehensive strategies for paclitaxel production: insights from plant cell culture, endophytic microorganisms, and synthetic biology
10.1093/hr/uhae346 · 2025 · External reference
miR5298b regulated taxol biosynthesis by acting on TcNPR3, resulting in an alleviation of the strong inhibition of the TcNPR3-TcTGA6 complex in Taxus chinensis
10.1016/j.ijbiomac.2023.125909 · 2023 · External reference
Role of an endodermis-specific miR858b-MYB1L module in the regulation of Taxol biosynthesis in Taxus mairei
10.1111/tpj.70135 · 2025 · External reference
Tissue-specific study across the stem of Taxus media identifies a phloem-specific TmMYB3 involved in the transcriptional regulation of paclitaxel biosynthesis
10.1111/tpj.14710 · 2020 · External reference
Improving the expression of taxadiene synthase to enhance the titer of taxadiene in Saccharomyces cerevisiae
10.1039/d4gc03079f · 2024 · External reference
Toward sustainable paclitaxel bioproduction: plant biology, biosynthesis and platform engineering
2026 · External reference
Two jasmonate-responsive factors, TcERF12 and TcERF15, respectively act as repressor and activator of tasy gene of taxol biosynthesis in Taxus chinensis
10.1007/s11103-015-0382-2 · 2015 · External reference
Computational redesign of taxane-10β-hydroxylase for de novo biosynthesis of a key paclitaxel intermediate
10.1007/s00253-023-12784-x · 2023 · External reference
Research advances in clinical applications, anticancer mechanism, total chemical synthesis, semi-synthesis and biosynthesis of paclitaxel
2023 · External reference
Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis
10.1016/j.molp.2023.10.016 · 2023 · External reference
Oxetane ring formation in Taxol biosynthesis is catalyzed by a bifunctional cytochrome P450 enzyme
10.1021/jacs.3c10864 · 2024 · External reference
Parameter optimization of ultrasonic-microwave synergistic extraction of taxanes from Taxus cuspidata needles
10.3390/molecules28237746 · 2023 · External reference
Engineering cyanobacteria as a new platform for producing taxol precursors directly from carbon dioxide
10.1186/s13068-024-02555-9 · 2024 · External reference
Distributing a metabolic pathway among a microbial consortium enhances production of natural products
10.1038/nbt.3095 · 2015 · External reference
High light promotes salicylic acid–dependent diterpenoid lactone biosynthesis in Andrographis paniculate
10.1016/j.ncrops.2025.100094 · 2026 · External reference