Research graph
References from Advances in directed evolution: Mutagenesis, sensing, screening, and in silico innovation. Local targets link to admitted publications; unresolved targets remain external evidence.
From self-replication to replicator systems en route to de novo life
10.1038/s41570-020-0196-x · 2020 · External reference
Inducible directed evolution of complex phenotypes in bacteria
10.1093/nar/gkac094 · 2022 · External reference
In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
10.1038/s41467-020-20230-z · 2020 · External reference
Comparative analysis of deep mutational scanning datasets in enteroviruses A and B identifies functional divergence and therapeutic targets
10.1038/s41559-026-02993-8 · 2026 · External reference
CADEE: computer-aided directed evolution of enzymes
10.1107/s2052252516018017 · 2017 · External reference
Chemical production of mutations
10.1038/157302a0 · 1946 · External reference
Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site
10.1038/s41467-023-42098-5 · 2023 · External reference
Genomic language models: opportunities and challenges
10.1016/j.tig.2024.11.013 · 2025 · External reference
An adaptable platform for directed evolution in human cells
10.1021/jacs.8b10937 · 2018 · External reference
Low-N protein engineering with data-efficient deep learning
10.1038/s41592-021-01100-y · 2021 · External reference
Functional alignment of protein language models via reinforcement learning
2026 · External reference
CatPred: a comprehensive framework for deep learning in vitro enzyme kinetic parameters
10.1038/s41467-025-57215-9 · 2025 · External reference
Accelerating enzyme discovery and engineering with high-throughput screening
10.1039/d4np00031e · 2025 · External reference
Virus-free continuous directed evolution in human cells using somatic hypermutation
2026 · External reference
Genome modelling and design across all domains of life with evo 2
10.1038/s41586-026-10176-5 · 2026 · External reference
Tunable protein degradation in bacteria
10.1038/nbt.3053 · 2014 · External reference
BRENDA, the ELIXIR core data resource in 2021: new developments and updates
10.1093/nar/gkaa1025 · 2021 · External reference
Learning protein fitness landscapes with deep mutational scanning data from multiple sources
10.1016/j.cels.2023.07.003 · 2023 · External reference
Wearable flexible microfluidic sensing technologies
10.1038/s44222-023-00094-w · 2023 · External reference
Helicase-assisted continuous editing for programmable mutagenesis of endogenous genomes
10.1126/science.adn5876 · 2024 · External reference
Reconstruction, simulation and analysis of enzyme-constrained metabolic models using GECKO toolbox 3.0
10.1038/s41596-023-00931-7 · 2024 · External reference
Structural dynamics-guided engineering of a riboswitch RNA for evolving c-di-AMP synthases
10.1126/sciadv.adt8165 · 2025 · External reference
Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae
10.1038/s41467-023-44511-5 · 2024 · External reference
Sequence display enables large-scale sequence–activity datasets for rapid protein evolution
10.1038/s41587-026-03087-3 · 2026 · External reference
BartSmiles: generative masked language models for molecular representations
10.1021/acs.jcim.4c00512 · 2024 · External reference
Selective fluorescent labeling of cellular proteins and its biological applications
10.1039/d4cs00094c · 2024 · External reference
Accurate top protein variant discovery via low-N pick-and-validate machine learning
10.1016/j.cels.2024.01.002 · 2024 · External reference
High-throughput optimisation of protein secretion in yeast via an engineered biosensor
10.1016/j.tibtech.2024.11.010 · 2025 · External reference
A chimeric viral platform for directed evolution in mammalian cells
10.1038/s41467-025-59438-2 · 2025 · External reference
Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering
10.1038/s41467-021-21876-z · 2021 · External reference
In vivo continuous evolution of genes and pathways in yeast
10.1038/ncomms13051 · 2016 · External reference
Enzyme specificity prediction using cross-attention graph neural networks
10.1038/s41586-025-09697-2 · 2025 · External reference
A pseudovirus system enables deep mutational scanning of the full SARS-CoV-2 spike
10.1016/j.cell.2023.02.001 · 2023 · External reference
Spike deep mutational scanning helps predict success of SARS-CoV-2 clades
10.1038/s41586-024-07636-1 · 2024 · External reference
Global characterization of in vivo enzyme catalytic rates and their correspondence to in vitro kcat measurements
10.1073/pnas.1514240113 · 2016 · External reference
Directing evolution: the next revolution in drug discovery?
10.1038/nrd.2017.146 · 2017 · External reference
Accelerated adaptive laboratory evolution: A tool for evolutionary biotechnology
10.1016/j.biotechadv.2025.108741 · 2026 · External reference
A propeptide-based biosensor for the selective detection of vibrio cholerae using an environment-sensitive fluorophore
10.1016/j.chembiol.2022.09.003 · 2022 · External reference
The VEGAS platform is unsuitable for mammalian directed evolution
10.1021/acssynbio.2c00460 · 2022 · External reference
Miniaturizing and modifying natural proteins with Raygun
10.1038/s41586-026-10842-8 · 2026 · External reference
An orthogonal T7 replisome for continuous hypermutation and accelerated evolution in E. coli
10.1126/science.adp9583 · 2025 · External reference
Machine learning-guided co-optimization of fitness and diversity facilitates combinatorial library design in enzyme engineering
10.1038/s41467-024-50698-y · 2024 · External reference
Michaelis-Menten for single enzyme molecules
10.1038/nmeth0306-158b · 2006 · External reference
Phage-assisted evolution and protein engineering yield compact, efficient prime editors
10.1016/j.cell.2023.07.039 · 2023 · External reference
Mutagenic deamination of cytosine residues in DNA
10.1038/287560a0 · 1980 · External reference
Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
10.1038/s41467-020-17061-3 · 2020 · External reference
VEGAS as a platform for facile directed evolution in mammalian cells
10.1016/j.cell.2019.05.051 · 2019 · External reference
ADEVO: proof-of-concept of adenovirus-directed EVOlution by random peptide display on the fiber knob
2024 · External reference
A system for the continuous directed evolution of biomolecules
10.1038/nature09929 · 2011 · External reference
Capabilities and challenges for the use of fluorescent RNAs in RNA dynamics research
10.1016/j.tcb.2025.05.004 · 2026 · External reference
A CRISPR-guided mutagenic DNA polymerase strategy for the detection of antibiotic-resistant mutations in M. tuberculosis
10.1016/j.omtn.2022.07.004 · 2022 · External reference
Genuine directed evolution in test tube (GENie)
2026 · External reference
RAISE: a simple and novel method of generating random insertion and deletion mutations
10.1093/nar/gnj032 · 2006 · External reference
Ultrahigh-throughput enzyme engineering and discovery in in vitro compartments
10.1021/acs.chemrev.2c00910 · 2023 · External reference
A gasdermin-based life–death evolution system for reprogramming protease specificity
10.1038/s41589-025-02063-3 · 2026 · External reference
Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)
10.1073/pnas.1606927113 · 2016 · External reference
Light-directed evolution of dynamic, multi-state, and computational protein functionalities
10.1016/j.cell.2026.02.002 · 2026 · External reference
Thermophiles in the genomic era (2015–2025): a review on biodiversity, metagenome-assembled genomes, and future directions
10.1080/1040841x.2026.2614431 · 2026 · External reference
Advancing microbial production through artificial intelligence-aided biology
10.1016/j.biotechadv.2024.108399 · 2024 · External reference
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: current advances and future prospects
10.1016/j.biotechadv.2024.108319 · 2024 · External reference
Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis
10.1093/genetics/164.2.731 · 2003 · External reference
Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes
10.1016/j.tibtech.2022.03.004 · 2022 · External reference
Directed evolution of a TNA polymerase identifies independent paths to fidelity and catalysis
10.1038/s41467-025-67652-1 · 2025 · External reference
CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window
10.1038/s41586-018-0384-8 · 2018 · External reference
Recent progress and prospects of alkaline phosphatase biosensor based on fluorescence strategy
10.1016/j.bios.2019.111811 · 2020 · External reference
Optimization of the activity and biodegradability of ionizable lipids for mRNA delivery via directed chemical evolution
10.1038/s41551-024-01267-7 · 2024 · External reference
RNA control of reverse transcription in a diversity-generating retroelement
10.1038/s41586-024-08405-w · 2025 · External reference
Precise DNA base editing using AlphaFold3-based contact modelling
10.1038/s41586-026-10794-z · 2026 · External reference
PRIZM: Combining low-N data and zero-shot models to design enhanced protein variants
2026 · External reference
Simulating 500 million years of evolution with a language model
10.1126/science.ads0018 · 2025 · External reference
Ramanome technology platform for label-free screening and sorting of microbial cell factories at single-cell resolution
10.1016/j.biotechadv.2019.04.010 · 2019 · External reference
Directed evolution in mammalian cells
10.1038/s41592-021-01090-x · 2021 · External reference
A biosensor for the direct visualization of auxin
10.1038/s41586-021-03425-2 · 2021 · External reference
Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
10.1038/nmeth.4038 · 2016 · External reference
Efficient evolution of human antibodies from general protein language models
10.1038/s41587-023-01763-2 · 2024 · External reference
An AsCas12f-based compact genome-editing tool derived by deep mutational scanning and structural analysis
10.1016/j.cell.2023.08.031 · 2023 · External reference
Deep mutational scanning identifies Cas1 and Cas2 variants that enhance type II-A CRISPR-Cas spacer acquisition
10.1038/s41467-025-60925-9 · 2025 · External reference
Learning protein fitness models from evolutionary and assay-labeled data
10.1038/s41587-021-01146-5 · 2022 · External reference
Land plant evolution: from microbial interaction to horizontal gene transfer
10.1016/j.tplants.2025.10.002 · 2026 · External reference
High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs
10.1038/s41587-022-01410-2 · 2023 · External reference
A comprehensive assessment of ultraviolet-radiation-induced mutations in Flammulina filiformis using whole-genome resequencing
10.3390/jof10030228 · 2024 · External reference
Nickase fidelity drives EvolvR-mediated diversification in mammalian cells
10.1038/s41467-025-58414-0 · 2025 · External reference
Biosensor-guided discovery and engineering of metabolic enzymes
10.1016/j.biotechadv.2023.108251 · 2023 · External reference
Integrated Tn-seq and MAGE-assisted rapid genome engineering targeting in Escherichia coli
10.1016/j.tibtech.2025.10.009 · 2026 · External reference
High-throughput method characterizes hundreds of previously unknown antibiotic resistance mutations
10.1038/s41467-025-56050-2 · 2025 · External reference
The design and engineering of synthetic genomes
10.1038/s41576-024-00786-y · 2025 · External reference
Phage display and other peptide display technologies
10.1093/femsre/fuab052 · 2022 · External reference
Cell-free systems: a synthetic biology tool for rapid prototyping in metabolic engineering
10.1016/j.biotechadv.2025.108522 · 2025 · External reference
Virus-assisted directed evolution of enhanced suppressor tRNAs in mammalian cells
10.1038/s41592-022-01706-w · 2023 · External reference
Enhanced directed evolution in mammalian cells yields a hyperefficient pyrrolysyl tRNA for noncanonical amino acid mutagenesis
10.1002/anie.202316428 · 2024 · External reference
Evolving antibody response to SARS-CoV-2 antigenic shift from XBB to JN.1
10.1038/s41586-024-08315-x · 2025 · External reference
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies
10.1016/j.biotechadv.2023.108173 · 2023 · External reference
Large stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells
10.1038/s41592-023-01997-7 · 2023 · External reference
A general temperature-guided language model to design proteins of enhanced stability and activity
10.1126/sciadv.adr2641 · 2024 · External reference
Rapid in silico directed evolution by a protein language model with EVOLVEpro
10.1126/science.adr6006 · 2024 · External reference
Inducible plasmid copy number control for synthetic biology in commonly used E. coli strains
10.1038/s41467-022-34390-7 · 2022 · External reference
Biology and evolution of bacterial toxin–antitoxin systems
10.1038/s41579-021-00661-1 · 2022 · External reference
Physics-based modeling in the new era of enzyme engineering
10.1038/s43588-025-00788-8 · 2025 · External reference
Directing evolution of novel ligands by mRNA display
10.1039/d1cs00160d · 2021 · External reference
Self-assembling biomolecules for biosensor applications
10.1186/s40824-023-00466-8 · 2023 · External reference
Generative design of bacteriophages with genome language models
10.1126/science.aec2657 · 2026 · External reference
A vaccinia-based system for directed evolution of GPCRs in mammalian cells
10.1038/s41467-023-37191-8 · 2023 · External reference
De novo design of synthetic microbial genomes
10.1038/s44222-026-00410-0 · 2026 · External reference
Turnover number predictions for kinetically uncharacterized enzymes using machine and deep learning
10.1038/s41467-023-39840-4 · 2023 · External reference
Accelerated enzyme engineering by machine-learning guided cell-free expression
10.1038/s41467-024-55399-0 · 2025 · External reference
Machine learning guided cell-free expression maps the biochemical landscape of carbonic anhydrase
2026 · External reference
Optofluidic Raman-activated cell sorting for targeted genome retrieval or cultivation of microbial cells with specific functions
10.1038/s41596-020-00427-8 · 2021 · External reference
Control of protein stability by post-translational modifications
10.1038/s41467-023-35795-8 · 2023 · External reference
Directed evolution of LaccID for cell surface proximity labeling and electron microscopy
10.1038/s41589-025-01973-6 · 2025 · External reference
Tuning evolvability via plasmid copy number and regulatory architecture
10.1038/s41467-025-67995-9 · 2025 · External reference
Deep learning-based kcat prediction enables improved enzyme-constrained model reconstruction
10.1038/s41929-022-00798-z · 2022 · External reference
Growth-coupled high throughput selection for directed enzyme evolution
10.1016/j.biotechadv.2023.108238 · 2023 · External reference
Targeted genome-modification tools and their advanced applications in crop breeding
10.1038/s41576-024-00720-2 · 2024 · External reference
Evaluation of machine learning-assisted directed evolution across diverse combinatorial landscapes
2025 · External reference
Large language model for knowledge synthesis and AI-enhanced biomanufacturing
10.1016/j.tibtech.2025.02.008 · 2025 · External reference
Hairpin fuel-driven fluorogenic DNA aptamer amplifier for high-contrast imaging of microRNAs in living cells
10.1016/j.bios.2026.118616 · 2026 · External reference
SAGE-prot: scoring-assisted generative exploration for multi-objective protein design
10.1093/bib/bbaf585 · 2025 · External reference
Evolutionary-scale prediction of atomic-level protein structure with a language model
10.1126/science.ade2574 · 2023 · External reference
How artificial intelligence is reengineering protein engineering
10.1126/science.aec8444 · 2026 · External reference
Opportunities and challenges in design and optimization of protein function
10.1038/s41580-024-00718-y · 2024 · External reference
OMEGA-guided DNA polymerases enable random mutagenesis in a tunable window
10.1016/j.tibtech.2025.02.011 · 2025 · External reference
Directed evolution of Escherichia coli Nissle 1917 functioning as a methylotrophic chassis
10.1016/j.cej.2025.164608 · 2025 · External reference
Biomimetic co-immobilization of β-glucosidase, glucose oxidase, and horseradish peroxidase to construct a multi-enzyme biosensor for determination of amygdalin
10.1016/j.ijbiomac.2025.139868 · 2025 · External reference
LDOB: multi-dimensional programmable lactose-derived oligosaccharide biosensors
10.1016/j.bios.2026.118407 · 2026 · External reference
Omega-based mini prime- and base-editing systems in Escherichia coli
10.1016/j.tibtech.2026.02.002 · 2026 · External reference
Advances in in vivo continuous evolution technologies for strain development and enzyme engineering
10.1016/j.biotechadv.2026.108895 · 2026 · External reference
Modular determinants of cleavage preference in GIY-YIG nucleases revealed by block-based DNA shuffling and directed evolution
10.1093/nar/gkaf1430 · 2026 · External reference
Transcription factor-based biosensor: A molecular-guided approach for advanced biofuel synthesis
10.1016/j.biotechadv.2024.108339 · 2024 · External reference
ECNet is an evolutionary context-integrated deep learning framework for protein engineering
10.1038/s41467-021-25976-8 · 2021 · External reference
Orthogonal RNA replication enables directed evolution and Darwinian adaptation in mammalian cells
10.1038/s41589-024-01783-2 · 2025 · External reference
EvoAI enables extreme compression and reconstruction of the protein sequence space
10.1038/s41592-024-02504-2 · 2025 · External reference
Targeted protein evolution in the gut microbiome by diversity-generating retroelements
10.1126/science.adv2111 · 2026 · External reference
Advances in ultrahigh-throughput screening for directed enzyme evolution
10.1039/c8cs00981c · 2020 · External reference
Ultra-high-throughput absorbance-activated droplet sorting for enzyme screening at kilohertz frequencies
10.1021/acs.analchem.2c04144 · 2023 · External reference
Expanded MutaT7 toolkit efficiently and simultaneously accesses all possible transition mutations in bacteria
10.1093/nar/gkad003 · 2023 · External reference
Phage-assisted continuous and non-continuous evolution
10.1038/s41596-020-00410-3 · 2020 · External reference
Continuous evolution of SpCas9 variants compatible with non-G PAMs
10.1038/s41587-020-0412-8 · 2020 · External reference
Recent advances in antibiotic-free markers; novel technologies to enhance safe human food production in the world
10.1007/s12033-022-00609-7 · 2023 · External reference
In vivo hypermutation and continuous evolution
10.1038/s43586-022-00119-5 · 2022 · External reference
Droplet-based microfluidics
10.1038/s43586-023-00212-3 · 2023 · External reference
Artificial transmutation of the gene
10.1126/science.66.1699.84 · 1927 · External reference
Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs
10.1038/nbt0102-76 · 2002 · External reference
High-throughput steady-state enzyme kinetics measured in a parallel droplet generation and absorbance detection platform
10.1021/acs.analchem.2c03164 · 2022 · External reference
Sequence modeling and design from molecular to genome scale with Evo
10.1126/science.ado9336 · 2024 · External reference
Growth-coupled enzyme engineering through manipulation of redox cofactor regeneration
10.1016/j.biotechadv.2023.108102 · 2023 · External reference
Advances in AAV capsid engineering: integrating rational design, directed evolution and machine learning
10.1016/j.ymthe.2025.03.056 · 2025 · External reference
Highly multiplexed design of an allosteric transcription factor to sense new ligands
10.1038/s41467-024-54260-8 · 2024 · External reference
Prime editor-based high-throughput screening reveals functional synonymous mutations in human cells
10.1038/s41587-025-02710-z · 2026 · External reference
Machine learning for functional protein design
10.1038/s41587-024-02127-0 · 2024 · External reference
Probing the limits of genetic recoding using multi-omics-guided evolution
10.1038/s41467-026-74300-9 · 2026 · External reference
Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system
10.1038/s41564-026-02346-y · 2026 · External reference
Automated in vivo enzyme engineering accelerates biocatalyst optimization
10.1038/s41467-024-46574-4 · 2024 · External reference
Computation-aided designs enable developing auxotrophic metabolic sensors for wide-range glyoxylate and glycolate detection
10.1038/s41467-025-57407-3 · 2025 · External reference
Chemical language modeling with structured state space sequence models
10.1038/s41467-024-50469-9 · 2024 · External reference
Methods for the directed evolution of proteins
10.1038/nrg3927 · 2015 · External reference
Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing
10.1038/s41551-024-01227-1 · 2025 · External reference
Design and construction of a microfluidics workstation for high-throughput multi-wavelength fluorescence and transmittance activated droplet analysis and sorting
10.1038/s41596-022-00796-2 · 2023 · External reference
Using general algebra to model the directed evolution of an asexual population
2025 · External reference
Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo
10.1093/nar/gkaa1231 · 2021 · External reference
Rapidly inducible yeast surface display for antibody evolution with OrthoRep
10.1021/acssynbio.4c00370 · 2024 · External reference
RiNALMo: general-purpose RNA language models can generalize well on structure prediction tasks
10.1038/s41467-025-60872-5 · 2025 · External reference
Engineered base editors with reduced bystander editing through directed evolution
10.1038/s41587-025-02937-w · 2025 · External reference
Continuous evolution of gene libraries towards arbitrary functions reveals the versatility of biomolecular evolution in vivo
2026 · External reference
Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM
10.1038/s41589-023-01481-5 · 2024 · External reference
Persistent spectral theory-guided protein engineering
10.1038/s43588-022-00394-y · 2023 · External reference
DLTKcat: deep learning-based prediction of temperature-dependent enzyme turnover rates
10.1093/bib/bbad506 · 2024 · External reference
Directed evolution of engineered virus-like particles with improved production and transduction efficiencies
10.1038/s41587-024-02467-x · 2024 · External reference
CRISPR-assisted probiotic and in situ engineering of gut microbiota: a prospect to modification of metabolic disorders
10.1007/s12602-025-10561-y · 2026 · External reference
Ultra-high-throughput mapping of genetic design space
10.1038/s41586-025-09933-9 · 2026 · External reference
Self-driving laboratories to autonomously navigate the protein fitness landscape
10.1038/s44286-023-00002-4 · 2024 · External reference
An orthogonal DNA replication system in yeast
10.1038/nchembio.1439 · 2014 · External reference
Scalable, continuous evolution of genes at mutation rates above genomic error thresholds
10.1016/j.cell.2018.10.021 · 2018 · External reference
Evolutionary advantage of the diversity-generating retroelement hypermutating system
2026 · External reference
Highly mutagenic continuous evolution in E. Coli using a Φ29-based orthogonal replication system
10.1038/s41587-025-02944-x · 2026 · External reference
A synthetic methylotrophic Escherichia coli as a chassis for bioproduction from methanol
10.1038/s41929-024-01137-0 · 2024 · External reference
Recent advancements of fluorescent biosensors using semisynthetic probes
10.1016/j.bios.2023.115862 · 2024 · External reference
Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
10.1038/s41587-020-0453-z · 2020 · External reference
Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities
10.1038/s41467-020-19539-6 · 2020 · External reference
Continuous evolution of user-defined genes at 1 million times the genomic mutation rate
10.1126/science.adm9073 · 2024 · External reference
Diversity-generating retroelements for programmable targeted hypermutagenesis
10.1038/s41587-026-03078-4 · 2026 · External reference
The evolution of display technologies for antibody drug discovery
10.1016/j.tibtech.2026.04.021 · 2026 · External reference
Design of highly functional genome editors by modelling CRISPR–Cas sequences
10.1038/s41586-025-09298-z · 2025 · External reference
Directed evolution of small RNA-stabilizing motifs that improve prime-editing efficiency
10.1038/s41587-026-03123-2 · 2026 · External reference
Directed evolution of microbial communities
10.1146/annurev-biophys-101220-072829 · 2021 · External reference
The community-function landscape of microbial consortia
10.1016/j.cels.2022.12.011 · 2023 · External reference
Directed evolution of enzymatic silicon-carbon bond cleavage in siloxanes
10.1126/science.adi5554 · 2024 · External reference
High-speed fluorescence image–enabled cell sorting
10.1126/science.abj3013 · 2022 · External reference
Functional viromic screens uncover regulatory RNA elements
10.1016/j.cell.2023.06.007 · 2023 · External reference
Landscape profiling of PET depolymerases using a natural sequence cluster framework
10.1126/science.adp5637 · 2025 · External reference
Type IX class utilizing cell signals for organizing toxin-antitoxin systems
10.1016/j.tim.2026.03.004 · 2026 · External reference
Unsupervised evolution of protein and antibody complexes with a structure-informed language model
10.1126/science.adk8946 · 2024 · External reference
An orthogonal transcription mutation system generating all transition mutations for accelerated protein evolution in vivo
10.1038/s41467-025-61354-4 · 2025 · External reference
Synonymous mutations in representative yeast genes are mostly strongly non-neutral
10.1038/s41586-022-04823-w · 2022 · External reference
Co-transcriptional gene regulation in eukaryotes and prokaryotes
10.1038/s41580-024-00706-2 · 2024 · External reference
CRISPR-DNA polymerase assisted targeted mutagenesis for regulable laboratory evolution
2025 · External reference
Comparative performance evaluation of bisulfite- and enzyme-based DNA conversion methods
10.1186/s13148-025-01855-7 · 2025 · External reference
A generalized platform for artificial intelligence-powered autonomous enzyme engineering
10.1038/s41467-025-61209-y · 2025 · External reference
Recent advances on the spectroscopic characterization of microbial biofilms: A critical review
2022 · External reference
Low fidelity mutants in the O-helix of Thermus aquaticus DNA polymerase I
10.1074/jbc.272.17.11228 · 1997 · External reference
UniProt: the universal protein knowledgebase in 2023
10.1093/nar/gkac1052 · 2023 · External reference
Engineering highly active nuclease enzymes with machine learning and high-throughput screening
2025 · External reference
Engineered TnpB genome editors for plants and human cells identified by ribonucleoprotein mutational scanning
10.1038/s41587-026-03059-7 · 2026 · External reference
Engineered bacterial orthogonal DNA replication system for continuous evolution
10.1038/s41589-023-01387-2 · 2023 · External reference
Establishing a synthetic orthogonal replication system enables accelerated evolution in E. Coli
10.1126/science.adk1281 · 2024 · External reference
A portable orthogonal replication system enables continuous gene evolution near the biological speed limit
2026 · External reference
Targeted diversification in the S. Cerevisiae genome with CRISPR-guided DNA polymerase I
10.1021/acssynbio.0c00149 · 2020 · External reference
Rapid directed evolution guided by protein language models and epistatic interactions
10.1126/science.aea1820 · 2026 · External reference
Directed evolution drives the next generation of biocatalysts
10.1038/nchembio.203 · 2009 · External reference
A novel directed evolution platform for engineering chemically gated protein switches
10.1039/d5cc06861d · 2026 · External reference
Adaptive model-guided protein evolution with sparse data optimizes compact eukaryotic genome editors
10.1038/s41587-026-03272-4 · 2026 · External reference
Hierarchical regulation and adaptive evolution of higher plants under abiotic stress
10.1021/acs.jafc.5c09033 · 2026 · External reference
Positive dielectrophoresis–based Raman-activated droplet sorting for culture-free and label-free screening of enzyme function in vivo
10.1126/sciadv.abb3521 · 2020 · External reference
Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids
10.1038/s41467-023-42431-y · 2023 · External reference
Dynamic plasmid copy number control for synthetic biology
10.1016/j.tibtech.2023.08.004 · 2024 · External reference
Robust enzyme discovery and engineering with deep learning using CataPro
10.1038/s41467-025-58038-4 · 2025 · External reference
EnzymeTuning improves enzyme-constrained metabolic modeling and proteome abundance prediction through deep learning
10.1038/s41467-026-73744-3 · 2026 · External reference
Enhancing prime editor activity by directed protein evolution in yeast
10.1038/s41467-024-46107-z · 2024 · External reference
Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase
10.1126/science.adt5199 · 2025 · External reference
SABIO-RK: an updated resource for manually curated biochemical reaction kinetics
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