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References from In vitro selection of VNAR antibodies using bioinformatics-based library design and cDNA display. Local targets link to admitted publications; unresolved targets remain external evidence.
Inhibition of tau seeding by targeting tau nucleation core within neurons with a single domain antibody fragment
10.1016/j.ymthe.2022.01.009 · 2022 · External reference
A novel PD-L1-targeted shark VNAR single-domain-based CAR-T cell strategy for treating breast cancer and liver cancer
10.1016/j.omto.2022.02.015 · 2022 · External reference
A human single-domain antibody elicits potent antitumor activity by targeting an epitope in mesothelin close to the cancer cell surface
10.1158/1535-7163.mct-12-0731 · 2013 · External reference
Naturally occurring antibodies devoid of light chains
10.1038/363446a0 · 1993 · External reference
A new antigen receptor gene family that undergoes rearrangement and extensive somatic diversification in sharks
10.1038/374168a0 · 1995 · External reference
Intraocular penetration of a vNAR: in vivo and In vitro VEGF165 neutralization
10.3390/md16040113 · 2018 · External reference
The structural analysis of shark IgNAR antibodies reveals evolutionary principles of immunoglobulins
10.1073/pnas.1321502111 · 2014 · External reference
Refined structure of an intact IgG2a monoclonal antibody
10.1021/bi962514+ · 1997 · External reference
Solution structure of a llama single-domain antibody with hydrophobic residues typical of the VH/VL interface
10.1021/bi012169a · 2002 · External reference
The influence of antibody humanization on shark variable domain (VNAR) binding site ensembles
10.3389/fimmu.2022.953917 · 2022 · External reference
Structural insights into epitope-paratope interactions of a monoclonal antibody targeting CEACAM5-expressing tumors
10.1038/s41467-024-53746-9 · 2024 · External reference
Mechanisms of SARS-CoV-2 neutralization by shark variable new antigen receptors elucidated through X-ray crystallography
10.1038/s41467-021-27611-y · 2021 · External reference
Therapeutic potential of Shark anti-ICOSL VNAR domains is exemplified in a murine model of autoimmune non-infectious uveitis
10.3389/fimmu.2017.01121 · 2017 · External reference
Blood-brain barrier transport using a high affinity, brain-selective VNAR antibody targeting transferrin receptor 1
10.1096/fj.202001787r · 2021 · External reference
An anti-hTNF-α variable new antigen receptor format demonstrates superior in vivo preclinical efficacy to Humira® in a transgenic mouse autoimmune polyarthritis disease model
10.3389/fimmu.2019.00526 · 2019 · External reference
Shark nanobodies with potent SARS-CoV-2 neutralizing activity and broad sarbecovirus reactivity
10.1038/s41467-023-36106-x · 2023 · External reference
VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica)
10.3389/fbioe.2023.1265582 · 2023 · External reference
A shark-derived broadly neutralizing nanobody targeting a highly conserved epitope on the S2 domain of sarbecoviruses
10.1186/s12951-025-03150-2 · 2025 · External reference
Cancer therapy via neoepitope-specific monoclonal antibody cocktails
10.1007/s00262-025-04075-3 · 2025 · External reference
Searching for peptide ligands with an epitope library
1990 · External reference
Construction and next-generation sequencing analysis of a large phage-displayed VNAR single-domain antibody library from six naïve nurse sharks
2019 · External reference
A single-domain VNAR nanobody binds with high-affinity and selectivity to the Heparin pentasaccharide fondaparinux
10.3390/ijms26094045 · 2025 · External reference
Construction of an artificially randomized IgNAR phage display library: screening of variable regions that bind to hen egg white lysozyme
10.1007/s10126-012-9456-1 · 2013 · External reference
A novel shark single-domain antibody targeting OGT as a tool for detection and intracellular localization
10.3389/fimmu.2023.1062656 · 2023 · External reference
Generation of a large peptide phage display library by self-ligation of whole-plasmid PCR product
10.1021/acschembio.0c00497 · 2020 · External reference
In vitro virus: bonding of mRNA bearing puromycin at the 3′-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro
10.1016/s0014-5793(97)01026-0 · 1997 · External reference
RNA-peptide fusions for the in vitro selection of peptides and proteins
10.1073/pnas.94.23.12297 · 1997 · External reference
cDNA display: a novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA-protein fusions
10.1093/nar/gkp514 · 2009 · External reference
Interleukin-17A peptide aptamers with an unexpected binding moiety selected by cDNA display under heterogenous conditions
10.1021/acsmedchemlett.1c00217 · 2021 · External reference
One-pot preparation of mRNA/cDNA display by a novel and versatile puromycin-linker DNA
10.1021/co2000295 · 2011 · External reference
A versatile puromycin-linker using cnvK for high-throughput in vitro selection by cDNA display
10.1016/j.jbiotec.2015.08.020 · 2015 · External reference
Comprehensive analysis of transglutaminase substrate preference by cDNA display coupled with next-generation sequencing and bioinformatics
10.1038/s41598-022-17494-4 · 2022 · External reference
Selection of peptides that associate with dye-conjugated solid surfaces in a pH-dependent manner using cDNA display
10.1021/acsomega.9b00631 · 2019 · External reference
A novel agonist with homobivalent single-domain antibodies that bind the FGF receptor 1 domain III functions as an FGF2 ligand
10.1016/j.jbc.2022.102804 · 2023 · External reference
A novel immuno-PCR method using cDNA display
10.1016/j.ab.2019.04.017 · 2019 · External reference
Anti-survivin single-domain antibodies derived from an artificial library including three synthetic random regions by in vitro selection using cDNA display
10.1016/j.bbrc.2018.07.158 · 2018 · External reference
Transcriptomic analysis of immunoglobulin novel antigen receptor (IgNAR) heavy chain constant domains of brownbanded bamboo shark (Chiloscyllium punctatum)
10.1016/j.fsi.2018.10.004 · 2019 · External reference
Crystal structure of a shark single-domain antibody V region in complex with lysozyme
2004 · External reference
Structure of a shark IgNAR antibody variable domain and modeling of an early-developmental isotype
10.1110/ps.051709505 · 2005 · External reference
Shark Attack: high affinity binding proteins derived from shark vNAR domains by stepwise in vitro affinity maturation
10.1016/j.jbiotec.2014.04.023 · 2014 · External reference
A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum
10.1038/nmeth.2413 · 2013 · External reference
Engineering of mCherry variants with long stokes shift, red-shifted fluorescence, and low cytotoxicity
2017 · External reference
Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein
10.1093/nar/24.22.4592 · 1996 · External reference
Accurate structure prediction of biomolecular interactions with AlphaFold 3
10.1038/s41586-024-07487-w · 2024 · External reference
Highly accurate protein structure prediction for the human proteome
10.1038/s41586-021-03828-1 · 2021 · External reference
Modulation of protein properties in living cells using nanobodies
10.1038/nsmb.1727 · 2010 · External reference
Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification
10.1038/s41598-020-62606-7 · 2020 · External reference
MEGA12: molecular evolutionary genetic analysis version 12 for adaptive and green computing
10.1093/molbev/msae263 · 2024 · External reference
The characterization of variable new antigen receptors targeting FAP isolated from a novel immunized library
10.1038/s42003-025-08610-x · 2025 · External reference
Importance of hypervariable region 2 for stability and affinity of a shark single-domain antibody specific for Ebola virus nucleoprotein
10.1371/journal.pone.0160534 · 2016 · External reference
Isolation and characterization of malaria PfHRP2 specific VNAR antibody fragments from immunized shark phage display library
10.1186/s12936-018-2531-y · 2018 · External reference
A high performance platform based on cDNA display for efficient synthesis of protein fusions and accelerated directed evolution
10.1021/acscombsci.5b00139 · 2016 · External reference
Atypical antigen recognition mode of a shark immunoglobulin new antigen receptor (IgNAR) variable domain characterized by humanization and structural analysis
10.1074/jbc.m112.435289 · 2013 · External reference
Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface
1985 · External reference
A humanized trivalent Nectin-4-targeting nanobody drug conjugate displays potent antitumor activity in gastric cancer
10.1186/s12951-024-02521-5 · 2024 · External reference
