Research graph
References from SPARCS enables scalable recovery of complex image-based phenotypes for genetic screening. Local targets link to admitted publications; unresolved targets remain external evidence.
The genetics of Caenorhabditis elegans
10.1093/genetics/77.1.71 · 1974 · External reference
Mutations affecting segment number and polarity in Drosophila
10.1038/287795a0 · 1980 · External reference
Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling
10.1038/nature15541 · 2015 · External reference
CRISPR technology: A decade of genome editing is only the beginning
10.1126/science.add8643 · 2023 · External reference
High-throughput functional genomics using CRISPR–Cas9
10.1038/nrg3899 · 2015 · External reference
High-content CRISPR screening
10.1038/s43586-021-00093-4 · 2022 · External reference
A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response
10.1016/j.cell.2016.11.048 · 2016 · External reference
Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq
10.1016/j.cell.2016.11.039 · 2016 · External reference
Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens
10.1016/j.cell.2016.11.038 · 2016 · External reference
Pooled CRISPR screening with single-cell transcriptome readout
10.1038/nmeth.4177 · 2017 · External reference
Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq
10.1016/j.cell.2022.05.013 · 2022 · External reference
Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits
10.1016/j.cell.2025.02.012 · 2025 · External reference
Sequencing-free whole-genome spatial transcriptomics at single-molecule resolution
10.1016/j.cell.2025.09.006 · 2025 · External reference
High-content CRISPR activation screens identify synthetically lethal RNA-based mechanisms to sensitize cancer cells to targeted T cell cytotoxicity
10.1038/s41588-026-02561-7 · 2026 · External reference
SPACE: multimodal spatial CRISPR screening with whole-transcriptome readout at subcellular resolution in 3D models
2026 · External reference
Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens
10.1016/j.cell.2018.09.022 · 2018 · External reference
Imaging-based screens of pool-synthesized cell libraries
10.1038/s41592-020-01053-8 · 2021 · External reference
Spatial CRISPR genomics identifies regulators of the tumor microenvironment
10.1016/j.cell.2022.02.015 · 2022 · External reference
Coupled Single-Cell CRISPR Screening and Epigenomic Profiling Reveals Causal Gene Regulatory Networks
10.1016/j.cell.2018.11.022 · 2019 · External reference
Multiome Perturb-seq unlocks scalable discovery of integrated perturbation effects on the transcriptome and epigenome
2025 · External reference
Scaling perturbations: beyond genome-scale CRISPR screens
2026 · External reference
Genome-wide single-cell perturbation screens with VIPerturb-seq
2026 · External reference
A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks
10.1016/j.cell.2015.06.059 · 2015 · External reference
Genome-scale CRISPR-Cas9 knockout screening in human cells
10.1126/science.1247005 · 2014 · External reference
Genetic screens in human cells using the CRISPR-Cas9 system
10.1126/science.1246981 · 2014 · External reference
Human NLRP1 is a sensor for double-stranded RNA
10.1126/science.abd0811 · 2021 · External reference
Microscopy-Based High-Content Screening
10.1016/j.cell.2015.11.007 · 2015 · External reference
Subcellular localization as a driver of protein function
10.1038/s41580-026-00947-3 · 2026 · External reference
Morphology and gene expression profiling provide complementary information for mapping cell state
10.1016/j.cels.2022.10.001 · 2022 · External reference
Single-cell and multivariate approaches in genetic perturbation screens
10.1038/nrg3768 · 2015 · External reference
Genetic and molecular architecture of complex traits
10.1016/j.cell.2024.01.023 · 2024 · External reference
Optical Pooled Screens in Human Cells
10.1016/j.cell.2019.09.016 · 2019 · External reference
The phenotypic landscape of essential human genes
10.1016/j.cell.2022.10.017 · 2022 · External reference
Multiplexed, image-based pooled screens in primary cells and tissues with PerturbView
10.1038/s41587-024-02391-0 · 2025 · External reference
NIS-Seq enables cell-type-agnostic optical perturbation screening
10.1038/s41587-024-02516-5 · 2025 · External reference
Mapping multimodal phenotypes to perturbations in cells and tissue with CRISPRmap
10.1038/s41587-024-02386-x · 2025 · External reference
The one-week automated genome-wide optical pooled screen
2026 · External reference
Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects
10.1016/j.cell.2026.04.031 · 2026 · External reference
A genome-wide optical pooled screen reveals regulators of cellular antiviral responses
10.1073/pnas.2210623120 · 2023 · External reference
A genome-wide atlas of human cell morphology
10.1038/s41592-024-02537-7 · 2025 · External reference
A pooled Cell Painting CRISPR screening platform enables de novo inference of gene function by self-supervised deep learning
10.1038/s41467-025-66778-6 · 2025 · External reference
Scalable multimodal mapping of macrophage regulatory architecture by integrating optical and transcriptomic pooled screens
2026 · External reference
Intelligent Image-Activated Cell Sorting
10.1016/j.cell.2018.08.028 · 2018 · External reference
High-speed fluorescence image-enabled cell sorting
10.1126/science.abj3013 · 2022 · External reference
Real-time pooled optical screening with single-cell isolation capability
2023 · External reference
Germinal center dynamics revealed by multiphoton microscopy with a photoactivatable fluorescent reporter
10.1016/j.cell.2010.10.032 · 2010 · External reference
Image-based pooled whole-genome CRISPRi screening for subcellular phenotypes
10.1083/jcb.202006180 · 2021 · External reference
High-content imaging-based pooled CRISPR screens in mammalian cells
10.1083/jcb.202008158 · 2021 · External reference
Versatile phenotype-activated cell sorting
10.1126/sciadv.abb7438 · 2020 · External reference
A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biology
10.1016/j.devcel.2025.10.015 · 2026 · External reference
High-throughput, microscope-based sorting to dissect cellular heterogeneity
10.15252/msb.20209442 · 2020 · External reference
Quantitative proteomic analysis of single pancreatic islets
10.1073/pnas.0908351106 · 2009 · External reference
A streamlined mass spectrometry-based proteomics workflow for large-scale FFPE tissue analysis
10.1002/path.5420 · 2020 · External reference
Deep Visual Proteomics defines single-cell identity and heterogeneity
10.1038/s41587-022-01302-5 · 2022 · External reference
Spatial single-cell mass spectrometry defines zonation of the hepatocyte proteome
10.1038/s41592-023-02007-6 · 2023 · External reference
Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
10.1038/s41586-025-08885-4 · 2025 · External reference
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
10.1038/nbt.3437 · 2016 · External reference
Mechanism and medical implications of mammalian autophagy
10.1038/s41580-018-0003-4 · 2018 · External reference
Autophagy genes in biology and disease
10.1038/s41576-022-00562-w · 2023 · External reference
Parametric UMAP Embeddings for Representation and Semi-supervised Learning
2020 · External reference
A subcellular map of the human proteome
10.1126/science.aal3321 · 2017 · External reference
C. elegans screen identifies autophagy genes specific to multicellular organisms
10.1016/j.cell.2010.04.034 · 2010 · External reference
Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites
10.1016/j.cell.2022.09.001 · 2022 · External reference
Molecular mechanisms and cellular functions of cGAS–STING signalling
10.1038/s41580-020-0244-x · 2020 · External reference
Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase
10.1016/j.cell.2013.04.046 · 2013 · External reference
Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA
10.1126/science.1229963 · 2013 · External reference
cGAS produces a 2'-5′-linked cyclic dinucleotide second messenger that activates STING
10.1038/nature12306 · 2013 · External reference
Autophagy induction via STING trafficking is a primordial function of the cGAS pathway
10.1038/s41586-019-1006-9 · 2019 · External reference
Innate immune sensing of cytosolic chromatin fragments through cGAS promotes senescence
10.1038/ncb3586 · 2017 · External reference
STING signaling modulation by COPII cargo recognition
10.1016/j.cell.2026.02.029 · 2026 · External reference
Mutations in COPA lead to abnormal trafficking of STING to the Golgi and interferon signaling
10.1084/jem.20200600 · 2020 · External reference
Deficiency in coatomer complex I causes aberrant activation of STING signalling
10.1038/s41467-022-29946-6 · 2022 · External reference
STING trafficking as a new dimension of immune signaling
10.1084/jem.20220990 · 2023 · External reference
Clathrin-associated AP-1 controls termination of STING signalling
10.1038/s41586-022-05354-0 · 2022 · External reference
Human STING is a proton channel
10.1126/science.adf8974 · 2023 · External reference
ArfGAP2 promotes STING proton channel activity, cytokine transit, and autoinflammation
10.1016/j.cell.2025.01.027 · 2025 · External reference
10.1038/s44319-023-00045-x · 2024 · Admitted local publication
Classification and functional characterization of regulators of intracellular STING trafficking identified by genome-wide optical pooled screening
10.1016/j.cels.2024.11.004 · 2024 · External reference
The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3
10.1016/j.cell.2017.09.039 · 2017 · External reference
Golgi apparatus-synthesized sulfated glycosaminoglycans mediate polymerization and activation of the cGAMP sensor STING
10.1016/j.immuni.2021.03.011 · 2021 · External reference
LRRC8A:C/E Heteromeric Channels Are Ubiquitous Transporters of cGAMP
10.1016/j.molcel.2020.10.021 · 2020 · External reference
SLC19A1 Is an Importer of the Immunotransmitter cGAMP
10.1016/j.molcel.2019.05.006 · 2019 · External reference
Design of amidobenzimidazole STING receptor agonists with systemic activity
10.1038/s41586-018-0705-y · 2018 · External reference
ESCRT-dependent STING degradation inhibits steady-state and cGAMP-induced signalling
10.1038/s41467-023-36132-9 · 2023 · External reference
YIPF5 Is Essential for Innate Immunity to DNA Virus and Facilitates COPII-Dependent STING Trafficking
10.4049/jimmunol.1900387 · 2019 · External reference
Single-cell spatial proteomics maps human liver zonation patterns and their vulnerability to disruption in tissue architecture
10.1038/s42255-026-01459-2 · 2026 · External reference
Predicting cellular responses to perturbation across diverse contexts with State
10.1016/j.cell.2026.07.052 · 2026 · External reference
GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus
10.1038/ncb1773 · 2008 · External reference
The discovery of potent small molecule activators of human STING
10.1016/j.ejmech.2020.112869 · 2021 · External reference
Activation of STING by targeting a pocket in the transmembrane domain
10.1038/s41586-022-04559-7 · 2022 · External reference
STING-induced noncanonical autophagy regulates endolysosomal homeostasis
2025 · External reference
Assembly of the yeast vacuolar H+-ATPase occurs in the endoplasmic reticulum and requires a Vma12p/Vma22p assembly complex
10.1083/jcb.142.1.39 · 1998 · External reference
Genome-wide CRISPR screen identifies host dependency factors for influenza A virus infection
2020 · External reference
TMEM199 Deficiency Is a Disorder of Golgi Homeostasis Characterized by Elevated Aminotransferases, Alkaline Phosphatase, and Cholesterol and Abnormal Glycosylation
10.1016/j.ajhg.2015.12.011 · 2016 · External reference
IKKbeta primes inflammasome formation by recruiting NLRP3 to the trans-Golgi network
10.1016/j.immuni.2022.10.021 · 2022 · External reference
Human Monocytes Engage an Alternative Inflammasome Pathway
10.1016/j.immuni.2016.01.012 · 2016 · External reference
Homeostatic regulation of STING by retrograde membrane traffic to the ER
10.1038/s41467-020-20234-9 · 2021 · External reference
CLCC1 governs ER bilayer equilibration to maintain lipid homeostasis
10.1038/s41586-026-10161-y · 2026 · External reference
CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly
10.1038/s41586-025-10064-4 · 2026 · External reference
ER protein CLCC1 promotes nuclear envelope fusion in herpesviral and host processes
10.1038/s41467-025-65115-1 · 2025 · External reference
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium
1952 · External reference
Self-guarding of MORC3 enables virulence factor-triggered immunity
10.1038/s41586-021-04054-5 · 2021 · External reference
The itinerary of autophagosomes: from peripheral formation to kiss-and-run fusion with lysosomes
10.1111/j.1600-0854.2008.00701.x · 2008 · External reference
Robust and Bright Genetically Encoded Fluorescent Markers for Highlighting Structures and Compartments in Mammalian Cells
2020 · External reference
Improved vectors and genome-wide libraries for CRISPR screening
10.1038/nmeth.3047 · 2014 · External reference
Stitching and registering highly multiplexed whole-slide images of tissues and tumors using ASHLAR
10.1093/bioinformatics/btac544 · 2022 · External reference
Unresolved reference
External reference
Fiji: an open-source platform for biological-image analysis
10.1038/nmeth.2019 · 2012 · External reference
DIA-NN: neural networks and interference correction enable deep proteome coverage in high throughput
10.1038/s41592-019-0638-x · 2020 · External reference
scPortrait integrates single-cell images into multimodal modeling
2025 · External reference
SCANPY: large-scale single-cell gene expression data analysis
10.1186/s13059-017-1382-0 · 2018 · External reference
A ConvNet for the 2020s
2022 · External reference
Protein Contaminants Matter: Building Universal Protein Contaminant Libraries for DDA and DIA Proteomics
10.1021/acs.jproteome.2c00145 · 2022 · External reference
SciPy 1.0: fundamental algorithms for scientific computing in Python
10.1038/s41592-019-0686-2 · 2020 · External reference
PyTorch: an Imperative Style, High-Performance Deep Learning Library
2019 · External reference
Cellpose 2.0: how to train your own model
10.1038/s41592-022-01663-4 · 2022 · External reference
Synthesis of an arrayed sgRNA library targeting the human genome
10.1038/srep14987 · 2015 · External reference
HeLa-CCL2 cell heterogeneity studied by single-cell DNA and RNA sequencing
10.1371/journal.pone.0225466 · 2019 · External reference
