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References from LibGENiE – A bioinformatic pipeline for the design of information-enriched enzyme libraries. Local targets link to admitted publications; unresolved targets remain external evidence.
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Algorithm-aided engineering of aliphatic halogenase WelO5* for the asymmetric late-stage functionalization of soraphens
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A machine learning approach for reliable prediction of amino acid interactions and its application in the directed evolution of enantioselective enzymes
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Directed evolution of enantioselective enzymes: Iterative cycles of CASTing for probing protein-sequence space
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10.1002/cbic.200800298
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Hot spots for the protein engineering of Baeyer-Villiger monooxygenases
10.1016/j.biotechadv.2017.11.007 · 2018 · External reference
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Making enzymes suitable for organic chemistry by rational protein design
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Revealing substrate positioning dynamics in non-heme Fe(II)/αKG-dependent halogenases through spectroscopically guided simulation.
2021 · External reference
10.1093/protein/gzw015
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Focused rational iterative site-specific mutagenesis (FRISM)
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Unresolved reference
External reference
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DeepDDG: predicting the stability change of protein point mutations using neural networks
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10.1016/j.jmb.2021.166964
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10.1093/nar/gky417 · External reference
10.1016/j.molcel.2018.08.033
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Designed active-site library reveals thousands of functional GFP variants
10.1038/s41467-023-38099-z · 2023 · External reference
Recent advances in user-friendly computational tools to engineer protein function
10.1093/bib/bbaa150 · 2020 · External reference
10.1021/acscatal.0c05450
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Asymmetric cation-olefin monocyclization by engineered squalene–hopene cyclases
10.1002/anie.202108037 · 2021 · External reference
Prediction of protein mutational free energy: benchmark and sampling improvements increase classification accuracy
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Generation of large libraries of random mutants in bacillus subtilis by PCR-based plasmid multimerization
10.2144/97232rr01 · 1997 · External reference
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10.1073/pnas.0500729102 · 2005 · External reference
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10.1073/pnas.0510098103
10.1073/pnas.0510098103 · External reference
10.1021/bi9804028
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Structural and thermodynamic bases for the design of pure prolactin receptor antagonists: X-ray structure of Del1-9-G129R-hPRL
10.1074/jbc.m704364200 · 2007 · External reference
10.1074/jbc.m109.012054
10.1074/jbc.m109.012054 · External reference
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Relationship between stability and function for isolated domains of troponin C
10.1021/bi961270q · 1996 · External reference
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10.1016/j.jmb.2005.07.066 · External reference
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10.1021/bi9726436 · 1998 · External reference
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10.1002/biot.200600170 · External reference
Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions
10.1039/b813388c · 2008 · External reference
Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction
10.2144/000114017 · 2013 · External reference
10.1021/acssynbio.9b00411
10.1021/acssynbio.9b00411 · External reference
10.1038/nmeth.3223
10.1038/nmeth.3223 · External reference
A Method for user-defined mutagenesis by integrating oligo pool synthesis technology with nicking mutagenesis
10.21769/bioprotoc.3697 · 2020 · External reference
Unresolved reference
External reference
CLUE: A bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries
2020 · External reference
10.1093/nar/18.7.1687
10.1093/nar/18.7.1687 · External reference
10.1093/nar/26.7.1819
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Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
10.1371/journal.pone.0197547 · 2018 · External reference
10.1093/nar/gkz268
10.1093/nar/gkz268 · External reference
An antisymmetric neural network to predict free energy changes in protein variants
10.1088/1361-6463/abedfb · 2021 · External reference
MEDUSA: prediction of protein flexibility from sequence
10.1016/j.jmb.2021.166882 · 2021 · External reference
10.1016/j.sbi.2021.11.001
10.1016/j.sbi.2021.11.001 · External reference
CAVER 3.0: a tool for the analysis of transport pathways in dynamic protein structures
10.1371/journal.pcbi.1002708 · 2012 · External reference
10.1093/nar/gkv315
10.1093/nar/gkv315 · External reference
FireProtASR: a web server for fully automated ancestral sequence reconstruction
10.1093/bib/bbaa337 · 2020 · External reference
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10.1126/science.ade2574 · External reference
A deep-learning sequence-based method to predict protein stability changes upon genetic variations
10.3390/genes12060911 · 2021 · External reference
Construction of stabilized proteins by combinatorial consensus mutagenesis
2004 · External reference
10.1002/prot.21670
10.1002/prot.21670 · External reference
Stabilizing proteins from sequence statistics: the interplay of conservation and correlation in triosephosphate isomerase stability
10.1016/j.jmb.2012.04.025 · 2012 · External reference
10.1016/j.sbi.2015.09.002
10.1016/j.sbi.2015.09.002 · External reference
Sequence statistics reliably predict stabilizing mutations in a protein domain
10.1006/jmbi.1994.1434 · 1994 · External reference
The consensus concept for thermostability engineering of proteins: further proof of concept
10.1093/protein/15.5.403 · 2002 · External reference
10.1093/nar/gkw416
10.1093/nar/gkw416 · External reference
10.1016/j.biotechadv.2013.10.012
10.1016/j.biotechadv.2013.10.012 · External reference
10.1093/protein/gzq071
10.1093/protein/gzq071 · External reference
Increasing the thermostability of sucrose phosphorylase by a combination of sequence- and structure-based mutagenesis
10.1093/protein/gzr042 · 2011 · External reference
Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method
2010 · External reference
10.1021/acs.chemrev.8b00290
10.1021/acs.chemrev.8b00290 · External reference
10.1038/nprot.2007.72
10.1038/nprot.2007.72 · External reference
10.1002/anie.201901491
10.1002/anie.201901491 · External reference
3DM: Systematic analysis of heterogeneous superfamily data to discover protein functionalities
2010 · External reference
Highly multiplexed, fast and accurate nanopore sequencing for verification of synthetic DNA constructs and sequence libraries
10.1093/synbio/ysz025 · 2019 · External reference