Research graph
References from Next-generation sequencing: A powerful multi-purpose tool in cell line development for biologics production. Local targets link to admitted publications; unresolved targets remain external evidence.
Screening strategies for high-yield chinese hamster ovary cell clones
10.3389/fbioe.2022.858478 · 2022 · External reference
Selection of transfected mammalian cells
2003 · External reference
Stable transfer of genes into mammalian cells
2001 · External reference
Selection of transfected mammalian cells
10.1002/0471142727.mb0905s86 · 2009 · External reference
Accelerating and de-risking CMC development with transposon-derived manufacturing cell lines
10.1002/bit.27742 · 2021 · External reference
Progress of transposon vector system for production of recombinant therapeutic proteins in mammalian cells
10.3389/fbioe.2022.879222 · 2022 · External reference
10.1038/s41587-022-01582-x
10.1038/s41587-022-01582-x · External reference
The genetics of somatic mammalian cells
10.1016/b978-1-4832-3111-2.50006-7 · 1957 · External reference
CHO Quasispecies—Implications for Manufacturing Processes
10.3390/pr1030296 · 2013 · External reference
Naming CHO cells for bio-manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names
10.1002/biot.202100165 · 2021 · External reference
CHO cells in biotechnology for production of recombinant proteins: current state and further potential
10.1007/s00253-011-3758-5 · 2012 · External reference
10.1002/bit.20865
10.1002/bit.20865 · External reference
Restoration of DNA repair mitigates genome instability and increases productivity of Chinese hamster ovary cells
10.1002/bit.28016 · 2022 · External reference
Growth rate changes in CHO host cells are associated with karyotypic heterogeneity
10.1002/biot.201700230 · 2018 · External reference
Recurring genomic structural variation leads to clonal instability and loss of productivity
10.1002/bit.26823 · 2019 · External reference
Identifying low-level sequence variants via next generation sequencing to aid stable CHO cell line screening
10.1002/btpr.2119 · 2015 · External reference
The fickle CHO: a review of the causes, implications, and potential alleviation of the CHO cell line instability problem
10.1016/j.copbio.2019.01.011 · 2019 · External reference
10.1016/j.tibtech.2016.02.013
10.1016/j.tibtech.2016.02.013 · External reference
Application of next generation sequencing in laboratory medicine
10.3343/alm.2021.41.1.25 · 2021 · External reference
A next-generation sequencing primer-how does it work and what can it do?
10.1177/2374289518766521 · 2018 · External reference
10.3390/biology12070997
10.3390/biology12070997 · External reference
10.1038/s41592-022-01730-w
10.1038/s41592-022-01730-w · External reference
Next-generation sequencing technologies: an overview
10.1016/j.humimm.2021.02.012 · 2021 · External reference
Overview of next-generation sequencing technologies
10.1002/cpmb.59 · 2018 · External reference
Unresolved reference
External reference
Validation of a next generation sequencing method for adventitious virus detection: Demonstration of sensitivity in multiple cell lines
2024 · External reference
Report of the third conference on next-generation sequencing for adventitious virus detection in biologics for humans and animals
2023 · External reference
A multicenter study to evaluate the performance of high-throughput sequencing for virus detection
10.1128/msphere.00307-17 · 2017 · External reference
10.3390/v10100566
10.3390/v10100566 · External reference
Evaluation of next-generation sequencing performance for in vitro detection of viruses in biological products
2024 · External reference
FDA’s activities supporting regulatory application of ‘next gen’ sequencing technologies
10.5731/pdajpst.2014.01024 · 2014 · External reference
10.1002/bit.27432
10.1002/bit.27432 · External reference
10.1038/nbt.2624
10.1038/nbt.2624 · External reference
10.1038/nbt.1932
10.1038/nbt.1932 · External reference
10.1002/bit.28226
10.1002/bit.28226 · External reference
10.1038/nbt.2645
10.1038/nbt.2645 · External reference
10.1002/bit.26722
10.1002/bit.26722 · External reference
Genomic sequencing and analysis of a Chinese hamster ovary cell line using Illumina sequencing technology
10.1186/1471-2164-12-67 · 2011 · External reference
Sequencing the CHO DXB11 genome reveals regional variations in genomic stability and haploidy
10.1186/s12864-015-1391-x · 2015 · External reference
10.1007/s00216-016-9549-1
10.1007/s00216-016-9549-1 · External reference
10.1093/nar/gkae332
10.1093/nar/gkae332 · External reference
Complete sequence verification of plasmid DNA using the Oxford Nanopore Technologies’ MinION device
10.1186/s12859-023-05226-y · 2023 · External reference
Multiplexed long-read plasmid validation and analysis using OnRamp
10.1101/gr.277369.122 · 2023 · External reference
Highly sensitive detection of mutations in CHO cell recombinant DNA using multi-parallel single molecule real-time DNA sequencing
10.1002/bit.26561 · 2018 · External reference
DetectIS: a pipeline to rapidly detect exogenous DNA integration sites using DNA or RNA paired-end sequencing data
10.1093/bioinformatics/btab366 · 2021 · External reference
10.1038/nbt.2959
10.1038/nbt.2959 · External reference
Targeted locus amplification and next-generation sequencing
10.1007/978-1-4939-6442-0_13 · 2017 · External reference
Structural analysis of random transgene integration in CHO manufacturing cell lines by targeted sequencing
10.1002/bit.28012 · 2022 · External reference
Multiplexed clonality verification of cell lines for protein biologic production
10.1002/btpr.2978 · 2020 · External reference
CRISPR/CAS9 targeted CAPTURE of mammalian genomic regions for characterization by NGS
10.1038/s41598-019-39667-4 · 2019 · External reference
10.1002/bit.28382
10.1002/bit.28382 · External reference
Deciphering integration loci of CHO manufacturing cell lines using long read nanopore sequencing
10.1016/j.nbt.2023.03.003 · 2023 · External reference
Analytical assessment of clonal derivation of eukaryotic/CHO cell populations
10.1016/j.jbiotec.2018.08.020 · 2018 · External reference
Genome-wide analysis of single nucleotide variants allows for robust and accurate assessment of clonal derivation in cell lines used to produce biologics
10.1002/bit.27534 · 2020 · External reference
10.1016/j.ymben.2017.02.001
10.1016/j.ymben.2017.02.001 · External reference
Investigation of metabolic variability observed in extended fed batch cell culture
10.1002/btpr.1787 · 2013 · External reference
A Consensus genome-scale reconstruction of chinese hamster ovary cell metabolism
10.1016/j.cels.2016.10.020 · 2016 · External reference
10.1016/j.nbt.2023.12.007
10.1016/j.nbt.2023.12.007 · External reference
A compendium of stable hotspots in the CHO genome
10.1002/bit.28390 · 2023 · External reference
10.1002/bit.27599
10.1002/bit.27599 · External reference
Context-dependent genomic locus effects on antibody production in recombinant Chinese hamster ovary cells generated through random integration
10.1016/j.csbj.2024.04.023 · 2024 · External reference
An online compendium of CHO RNA-seq data allows identification of CHO cell line-specific transcriptomic signatures
10.1002/biot.201800070 · 2018 · External reference
A global RNA-seq-driven analysis of CHO host and production cell lines reveals distinct differential expression patterns of genes contributing to recombinant antibody glycosylation
10.1002/biot.201400652 · 2015 · External reference
An RNA-seq based transcriptomic investigation into the productivity and growth variants with Chinese hamster ovary cells
10.1016/j.jbiotec.2018.02.008 · 2018 · External reference
10.1016/j.csbj.2024.12.006 · Admitted local publication
RNA-seq highlights high clonal variation in monoclonal antibody producing CHO cells
10.1002/biot.201700231 · 2018 · External reference
Subcloning induces changes in the DNA-methylation pattern of outgrowing Chinese hamster ovary cell colonies
10.1002/biot.202000350 · 2021 · External reference
Transcriptome analysis of a CHO cell line expressing a recombinant therapeutic protein treated with inducers of protein expression
10.1016/j.jbiotec.2015.08.025 · 2015 · External reference
Chemical and genetic modulation of complex I of the electron transport chain enhances the biotherapeutic protein production capacity of CHO cells
10.3390/cells12222661 · 2023 · External reference
Transcriptomic analysis reveals mode of action of butyric acid supplementation in an intensified CHO cell fed-batch process
10.1002/bit.28150 · 2022 · External reference
Differential gene expression of a feed-spiked super-producing CHO cell line
10.1016/j.jbiotec.2018.08.013 · 2018 · External reference
Transcriptomic analysis of IgG4 Fc-fusion protein degradation in a panel of clonally-derived CHO cell lines using RNASeq
10.1002/bit.26958 · 2019 · External reference
10.1016/s0092-8674(04)00045-5
10.1016/s0092-8674(04)00045-5 · External reference
10.1016/j.tibtech.2008.03.010
10.1016/j.tibtech.2008.03.010 · External reference
10.1016/j.jbiotec.2011.02.011
10.1016/j.jbiotec.2011.02.011 · External reference
10.1016/j.biotechadv.2013.07.007
10.1016/j.biotechadv.2013.07.007 · External reference
The effect of microRNA on the production of recombinant protein in CHO cells and its mechanism
10.3389/fbioe.2022.832065 · 2022 · External reference
10.1038/s41580-022-00566-8
10.1038/s41580-022-00566-8 · External reference
10.1038/s41580-020-00315-9
10.1038/s41580-020-00315-9 · External reference
Transcriptome-scale RNA-targeting CRISPR screens reveal essential lncRNAs in human cells
10.1016/j.cell.2024.10.021 · 2024 · External reference
10.1002/bit.27467
10.1002/bit.27467 · External reference
The long non-coding RNA transcriptome landscape in CHO cells under batch and fed-batch conditions
10.1002/biot.201800122 · 2018 · External reference
Defining lncRNAs correlated with CHO cell growth and IgG productivity by RNA-Seq
10.1016/j.isci.2019.100785 · 2020 · External reference
LncRNA analysis of mAb producing CHO clones reveals marker and engineering potential
10.1016/j.ymben.2023.05.003 · 2023 · External reference
10.1002/bit.27715
10.1002/bit.27715 · External reference
10.1016/j.jbiotec.2023.01.006
10.1016/j.jbiotec.2023.01.006 · External reference
10.1016/j.tibtech.2007.07.004
10.1016/j.tibtech.2007.07.004 · External reference
Towards next generation CHO cell biology: bioinformatics methods for RNA-Seq-based expression profiling
10.1002/biot.201500107 · 2015 · External reference
From observational to actionable: rethinking omics in biologics production
10.1016/j.tibtech.2023.03.009 · 2023 · External reference
10.1186/s13059-017-1215-1
10.1186/s13059-017-1215-1 · External reference
Multi-omics data deconvolution and integration: new methods, insights, and translational implications
10.1007/978-1-0716-2986-4_1 · 2023 · External reference
10.1002/mco2.315
10.1002/mco2.315 · External reference
Advancing personalized medicine by analytical means: selection of three
2024 · External reference
A comprehensive review of machine learning techniques for multi-omics data integration: challenges and applications in precision oncology
10.1093/bfgp/elae013 · 2024 · External reference
Integrated multi-omics for rapid rare disease diagnosis on a national scale
10.1038/s41591-023-02401-9 · 2023 · External reference
Mammalian systems biotechnology reveals global cellular adaptations in a recombinant CHO cell line
10.1016/j.cels.2017.04.009 · 2017 · External reference
CHOmics: a web-based tool for multi-omics data analysis and interactive visualization in CHO cell lines
10.1371/journal.pcbi.1008498 · 2020 · External reference
Multi-omics profiling of a CHO cell culture system unravels the effect of culture pH on cell growth, antibody titer, and product quality
10.1002/bit.27899 · 2021 · External reference
Multi-omic characterization of antibody-producing CHO cell lines elucidates metabolic reprogramming and nutrient uptake bottlenecks
10.1016/j.ymben.2024.07.009 · 2024 · External reference
Determination of critical quality attributes for monoclonal antibodies using quality by design principles
2016 · External reference
The confluence of innovation in therapeutics and regulation: recent CMC considerations
10.1016/j.xphs.2020.09.025 · 2020 · External reference
Unresolved reference
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Unresolved reference
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Evolution of a comprehensive, orthogonal approach to sequence variant analysis for biotherapeutics
10.1080/19420862.2018.1531965 · 2019 · External reference
Genetic mutation analysis at early stages of cell line development using next generation sequencing
10.1002/btpr.2263 · 2016 · External reference
Mutation detection in an antibody-producing chinese hamster ovary cell line by targeted RNA sequencing
2016 · External reference
10.1038/nmeth.2772
10.1038/nmeth.2772 · External reference
Correcting PCR amplification errors in unique molecular identifiers to generate accurate numbers of sequencing molecules
10.1038/s41592-024-02168-y · 2024 · External reference
High accuracy meets high throughput for near full-length 16S ribosomal RNA amplicon sequencing on the Nanopore platform
10.1093/pnasnexus/pgae411 · 2024 · External reference
Je, a versatile suite to handle multiplexed NGS libraries with unique molecular identifiers
10.1186/s12859-016-1284-2 · 2016 · External reference
AmpUMI: design and analysis of unique molecular identifiers for deep amplicon sequencing
10.1101/288118 · 2018 · External reference
Benefits and challenges with applying unique molecular identifiers in next generation sequencing to detect low frequency mutations
10.1371/journal.pone.0146638 · 2016 · External reference
Monoclonal antibody sequence variants disguised as fragments: identification, characterization, and their removal by purification process optimization
10.1016/j.xphs.2022.08.002 · 2022 · External reference
Identification and characterization of an IgG sequence variant with an 11 kDa heavy chain C-terminal extension using a combination of mass spectrometry and high-throughput sequencing analysis
10.1080/19420862.2019.1667740 · 2019 · External reference
10.1038/nrg.2017.44
10.1038/nrg.2017.44 · External reference
10.1038/nmeth.3144
10.1038/nmeth.3144 · External reference
10.1016/j.cell.2007.01.033
10.1016/j.cell.2007.01.033 · External reference
A mechanistic understanding of production instability in CHO cell lines expressing recombinant monoclonal antibodies
10.1002/bit.23189 · 2011 · External reference
Mechanisms underlying epigenetic and transcriptional heterogeneity in Chinese hamster ovary (CHO) cell lines
10.1186/s12896-016-0238-0 · 2016 · External reference
Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time
10.1002/bit.25990 · 2016 · External reference
10.1002/bit.26891
10.1002/bit.26891 · External reference
Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells
10.1016/j.ymben.2021.04.014 · 2021 · External reference
CRISPR/Cas9-mediated gene knockout for DNA methyltransferase Dnmt3a in CHO cells displays enhanced transgenic expression and long-term stability
10.1111/jcmm.13687 · 2018 · External reference
Enhancing expression level and stability of transgene mediated by episomal vector via buffering DNA methyltransferase in transfected CHO cells
10.1002/jcb.28835 · 2019 · External reference
10.1016/j.csbj.2020.11.008 · Admitted local publication
SnapShot: chromosome confirmation capture
10.1016/j.cell.2012.02.019 · 2012 · External reference
10.1038/s41588-019-0472-1
10.1038/s41588-019-0472-1 · External reference
10.1016/j.cell.2016.09.037
10.1016/j.cell.2016.09.037 · External reference
Transcription factories
10.3389/fgene.2012.00221 · 2012 · External reference
A Chinese hamster transcription start site atlas that enables targeted editing of CHO cells
2021 · External reference
Unresolved reference
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