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References from Overcoming lentiviral delivery limitations in hard-to-transduce suspension cells for genome-wide CRISPR screening. Local targets link to admitted publications; unresolved targets remain external evidence.
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Vesicular stomatitis virus G protein transmembrane region is crucial for the hemi-fusion to full fusion transition
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A guide in lentiviral vector production for hard-to-transfect cells, using cardiac-derived c-kit expressing cells as a model system
10.1038/s41598-021-98657-7 · 2021 · External reference
CRISPR: a versatile tool for both forward and reverse genetics research
10.1007/s00439-016-1704-4 · 2016 · External reference
Discovery of putative tumor suppressors from CRISPR screens reveals rewired lipid metabolism in acute myeloid leukemia cells
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CRISPR screens for functional interrogation of immunity
10.1038/s41577-022-00802-4 · 2023 · External reference
A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens
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A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes
10.1016/j.ymben.2021.03.017 · 2021 · External reference
Reprogramming AA catabolism in CHO cells with CRISPR/Cas9 genome editing improves cell growth and reduces byproduct secretion
10.1016/j.ymben.2019.09.005 · 2019 · External reference
Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool
10.1002/bit.25233 · 2014 · External reference
Discovery of target genes and pathways at GWAS loci by pooled single-cell CRISPR screens
10.1126/science.adh7699 · 2023 · External reference
Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function
10.1016/j.cell.2018.10.024 · 2018 · External reference
A Genome-wide CRISPR Screen Reveals a Role for the Non-canonical Nucleosome-Remodeling BAF Complex in Foxp3 Expression and Regulatory T Cell Function
10.1016/j.immuni.2020.06.011 · 2020 · External reference
Focused CRISPR-Cas9 genetic screening reveals USO1 as a vulnerability in B-cell acute lymphoblastic leukemia
10.1038/s41598-021-92448-w · 2021 · External reference
Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives
10.3390/v13071288 · 2021 · External reference
Production, purification and titration of a lentivirus-based vector for gene delivery purposes
10.1007/s10616-013-9652-5 · 2014 · External reference
CRISPR-Based Lentiviral Knockout Libraries for Functional Genomic Screening and Identification of Phenotype-Related Genes
2019 · External reference
Pois(s)on – It’s a Question of Dose
10.1038/sj.gt.3302270 · 2004 · External reference
High-content CRISPR screening
10.1038/s43586-021-00093-4 · 2022 · External reference
High-content CRISPR screening in tumor immunology
10.3389/fimmu.2022.1041451 · 2022 · External reference
Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening
10.1038/nprot.2017.016 · 2017 · External reference
Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines
10.1007/978-1-4939-9151-8_16 · 2019 · External reference
Research Techniques Made Simple: CRISPR Genetic Screens
10.1016/j.jid.2020.01.018 · 2020 · External reference
Insights into product and process related challenges of lentiviral vector bioprocessing
10.1002/bit.28498 · 2024 · External reference
Simplified production and concentration of lentiviral vectors to achieve high transduction in primary human T cells
10.1186/1472-6750-13-98 · 2013 · External reference
Systematic improvement of lentivirus transduction protocols by antibody fragments fused to VSV-G as envelope glycoprotein
10.1016/j.biomaterials.2014.01.051 · 2014 · External reference
Process for an efficient lentiviral cell transduction
10.1093/biomethods/bpaa005 · 2020 · External reference
Highly efficient ex vivo lentiviral transduction of primary human pancreatic exocrine cells
10.1038/s41598-019-51763-z · 2019 · External reference
Vector Copy Distribution at a Single-Cell Level Enhances Analytical Characterization of Gene-Modified Cell Therapies
10.1016/j.omtm.2020.04.016 · 2020 · External reference
Am I ready for CRISPR? A user’s guide to genetic screens
10.1038/nrg.2017.97 · 2018 · External reference
Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
10.1371/journal.pone.0197547 · 2018 · External reference
Correcting CRISPR for copy number
10.1038/ng.3994 · 2017 · External reference
Sources of Error in Mammalian Genetic Screens
10.1534/g3.116.030973 · 2016 · External reference
Improving recombinant protein production in CHO cells using the CRISPR-Cas system
10.1016/j.biotechadv.2023.108115 · 2023 · External reference
Secretion analysis of intracellular “difficult-to-express” immunoglobulin G (IgG) in Chinese hamster ovary (CHO) cells
10.1007/s10616-018-0286-5 · 2019 · External reference
The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line
10.1038/nbt.1932 · 2011 · External reference
Human cell lines for the production of recombinant proteins: on the horizon
10.1016/j.nbt.2014.11.005 · 2015 · External reference
Fed-batch production assessment of a tetravalent bispecific antibody: A case study on piggyBac stably transfected HEK293 cells
10.1016/j.nbt.2021.07.002 · 2021 · External reference
A Brain-Targeting Bispecific-Multivalent Antibody Clears Soluble Amyloid-Beta Aggregates in Alzheimer’s Disease Mice
10.1007/s13311-022-01283-y · 2022 · External reference
High-Throughput Dissection of AAV–Host Interactions: The Fast and the Curious
10.1016/j.jmb.2018.05.022 · 2018 · External reference
Production of lentiviral vectors
10.1038/mtm.2016.17 · 2016 · External reference
HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors
10.3389/fbioe.2021.796991 · 2021 · External reference
Affecting HEK293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes
10.3390/cells10071667 · 2021 · External reference
Trispecific antibodies produced from mAb2 pairs by controlled Fab-arm exchange
10.1515/hsz-2021-0376 · 2022 · External reference
Engineering Human Cells Expressing CRISPR/Cas9-Synergistic Activation Mediators for Recombinant Protein Production
10.3390/ijms24108468 · 2023 · External reference
CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering
10.3390/ijms24098144 · 2023 · External reference
Expanding the CRISPR toolbox for Chinese hamster ovary cells with comprehensive tools for Mad7 genome editing
10.1002/bit.28367 · 2023 · External reference
Virus susceptibility of Chinese hamster ovary (CHO) cells and detection of viral contaminations by adventitious agent testing
10.1002/bit.22723 · 2010 · External reference
Optimized Lentiviral Transduction Protocols by Use of a Poloxamer Enhancer, Spinoculation, and scFv-Antibody Fusions to VSV-G
2016 · External reference
Spinoculation Triggers Dynamic Actin and Cofilin Activity That Facilitates HIV-1 Infection of Transformed and Resting CD4 T Cells
10.1128/jvi.05170-11 · 2011 · External reference
Human Immunodeficiency Virus Type 1 Spinoculation Enhances Infection through Virus Binding
10.1128/jvi.74.21.10074-10080.2000 · 2000 · External reference
Spinoculation and retronectin highly enhance the gene transduction efficiency of Mucin-1-specific chimeric antigen receptor (CAR) in human primary T cells
10.1186/s12860-021-00397-z · 2021 · External reference
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10.1002/bit.21882 · 2008 · External reference
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Retroviral Infection Is Limited by Brownian Motion
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Influence of Microenvironment on Mesenchymal Stem Cell Therapeutic Potency: From Planar Culture to Microcarriers
10.3389/fbioe.2020.00640 · 2020 · External reference
High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2
10.1016/j.omtm.2019.01.005 · 2019 · External reference
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10.1007/s004170050129 · 1998 · External reference
A genome-scale CRISPR deletion screen in Chinese hamster ovary cells reveals essential regions of the coding and non-coding genome
10.1016/j.ymben.2025.08.007 · 2025 · External reference
The CRISPR tool kit for genome editing and beyond
10.1038/s41467-018-04252-2 · 2018 · External reference
CRISPR-dependent Base Editing Screens Identify Separation of Function Mutants of RADX with Altered RAD51 Regulatory Activity
10.1016/j.jmb.2023.168236 · 2023 · External reference
Recent advances in CRISPR/Cas9-mediated knock-ins in mammalian cells
10.1016/j.jbiotec.2019.11.010 · 2020 · External reference
Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9
10.1002/cpmb.100 · 2019 · External reference
Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection
10.1016/j.jbiotec.2015.04.024 · 2015 · External reference
An optimized genome-wide, virus-free CRISPR screen for mammalian cells
2021 · External reference
Enhanced Biosynthesis Performance of Heterologous Proteins in CHO-K1 Cells Using CRISPR-Cas9
10.1021/acssynbio.7b00375 · 2018 · External reference
Preselection of recombinant gene integration sites enabling high transcription rates in CHO cells using alternate start codons and recombinase mediated cassette exchange
10.1002/bit.26388 · 2017 · External reference
A new age in functional genomics using CRISPR/Cas9 in arrayed library screening
10.3389/fgene.2015.00300 · 2015 · External reference
Optimized Transgene Delivery Using Third-Generation Lentiviruses
10.1002/cpmb.125 · 2020 · External reference
Highly efficient large-scale lentiviral vector concentration by tandem tangential flow filtration
10.1016/j.jviromet.2011.06.019 · 2011 · External reference
Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators
10.1016/j.xpro.2023.102762 · 2023 · External reference
Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors
10.1038/nprot.2009.22 · 2009 · External reference
Production of Vesicular Stomatitis Virus G Glycoprotein (VSV-G) Pseudotyped Retroviral Vectors
2007 · External reference
