Research graph
References from Rational engineering of the P5 TRS-mimic site and REP78/68 start codon yields promoter variants that improve rAAV purity while maintaining high titers. Local targets link to admitted publications; unresolved targets remain external evidence.
rAAV immunogenicity, toxicity, and durability in 255 clinical trials: A meta-analysis
10.3389/fimmu.2022.1001263 · 2022 · External reference
Adeno-associated virus vector as a platform for gene therapy delivery
10.1038/s41573-019-0012-9 · 2019 · External reference
Good Manufacturing Practice Production of Self-Complementary Serotype 8 Adeno-Associated Viral Vector for a Hemophilia B Clinical Trial
10.1089/hum.2010.202 · 2011 · External reference
A novel 165-base-pair terminal repeat sequence is the sole cis requirement for the adeno-associated virus life cycle
10.1128/jvi.71.2.941-948.1997 · 1997 · External reference
The Use of Heterologous Promoters for Adeno-Associated Virus (AAV) Protein Expression in AAV Vector Production
10.1111/j.1348-0421.1998.tb02269.x · 1998 · External reference
Role for highly regulated rep gene expression in adeno-associated virus vector production
10.1128/jvi.71.7.5236-5243.1997 · 1997 · External reference
Negative regulation of the adeno-associated virus (AAV) P5 promoter involves both the P5 rep binding site and the consensus ATP-binding motif of the AAV Rep68 protein
10.1128/jvi.69.11.6787-6796.1995 · 1995 · External reference
The cellular transcription factor SP1 and an unknown cellular protein are required to mediate Rep protein activation of the adeno-associated virus p19 promoter
10.1128/jvi.71.3.1747-1756.1997 · 1997 · External reference
Positive and negative autoregulation of the adeno-associated virus type 2 genome
10.1128/jvi.60.1.251-258.1986 · 1986 · External reference
Studies of the Mechanism of Transactivation of the Adeno-Associated Virus p19 Promoter by Rep Protein
10.1128/jvi.76.16.8225-8235.2002 · 2002 · External reference
The adeno-associated virus type 2 p40 promoter requires a proximal Sp1 interaction and a p19 CArG-like element to facilitate Rep transactivation
10.1128/jvi.71.6.4300-4309.1997 · 1997 · External reference
Stowaways in the cargo: Contaminating nucleic acids in rAAV preparations for gene therapy
10.1016/j.ymthe.2023.07.025 · 2023 · External reference
Preventing packaging of translatable P5-associated DNA contaminants in recombinant AAV vector preps
10.1016/j.omtm.2022.01.008 · 2022 · External reference
Nanopore sequencing of native adeno-associated virus (AAV) single-stranded DNA using a transposase-based rapid protocol
2020 · External reference
Advanced Characterization of DNA Molecules in rAAV Vector Preparations by Single-stranded Virus Next-generation Sequencing
10.1038/mtna.2015.32 · 2015 · External reference
OneBac 2.0: Sf9 Cell Lines for Production of AAV5 Vectors with Enhanced Infectivity and Minimal Encapsidation of Foreign DNA
10.1089/hum.2015.050 · 2015 · External reference
An inducible system for highly efficient production of recombinant adeno-associated virus (rAAV) vectors in insect Sf9 cells
10.1073/pnas.0810614106 · 2009 · External reference
Direct ITR-to-ITR nanopore sequencing of AAV vector genomes
10.1089/hum.2022.143 · 2022 · External reference
Short DNA Hairpins Compromise Recombinant Adeno-Associated Virus Genome Homogeneity
10.1016/j.ymthe.2017.03.028 · 2017 · External reference
Extra-viral DNA in adeno-associated viral vector preparations induces TLR9-dependent innate immune responses in human plasmacytoid dendritic cells
10.1038/s41598-023-28830-7 · 2023 · External reference
Off-Target Expression of Cre-Dependent Adeno-Associated Viruses in Wild-Type C57BL/6J Mice
10.1523/eneuro.0363-21.2021 · 2021 · External reference
Characterization of residual microRNAs in AAV vector batches produced in HEK293 mammalian cells and Sf9 insect cells
10.1016/j.omtm.2024.101305 · 2024 · External reference
Comprehensive Comparison of AAV Purification Methods: Iodixanol Gradient Centrifugation vs. Immuno-Affinity Chromatography
10.1155/2023/2339702 · 2023 · External reference
Comparative Analysis of Cesium Chloride- and Iodixanol-Based Purification of Recombinant Adeno-Associated Viral Vectors for Preclinical Applications
10.1089/hgtb.2015.051 · 2015 · External reference
Human and Insect Cell-Produced Recombinant Adeno-Associated Viruses Show Differences in Genome Heterogeneity
10.1089/hum.2022.050 · 2022 · External reference
Toxicity after AAV delivery of RNAi expression constructs into nonhuman primate brain
10.1038/s41591-021-01522-3 · 2021 · External reference
Pre-Existing Immunity to a Nucleic Acid Contaminant-Derived Antigen Mediates Transaminitis and Resultant Diminished Transgene Expression in a Mouse Model of Hepatic Recombinant Adeno-Associated Virus-Mediated Gene Transfer
10.1089/hum.2023.188 · 2024 · External reference
Adeno-associated virus: from defective virus to effective vector
10.1186/1743-422x-2-43 · 2005 · External reference
547. AAV Preparations Contain Contamination from DNA Sequences in Production Plasmids Directly Outside of the ITRs
10.1016/s1525-0016(16)33355-x · 2016 · External reference
Mechanism of Rep-Mediated Adeno-Associated Virus Origin Nicking
10.1128/jvi.74.17.7762-7771.2000 · 2000 · External reference
Factors affecting the terminal resolution site endonuclease, helicase, and ATPase activities of adeno-associated virus type 2 Rep proteins
10.1128/jvi.73.10.8235-8244.1999 · 1999 · External reference
The Cellular TATA Binding Protein Is Required for Rep-Dependent Replication of a Minimal Adeno-Associated Virus Type 2 p5 Element
10.1128/jvi.79.17.11082-11094.2005 · 2005 · External reference
Four-Dimensional Visualization of the Simultaneous Activity of Alternative Adeno-Associated Virus Replication Origins
10.1128/jvi.79.19.12218-12230.2005 · 2005 · External reference
Rep-mediated nicking of the adeno-associated virus origin requires two biochemical activities, DNA helicase activity and transesterification
10.1128/jvi.73.11.9325-9336.1999 · 1999 · External reference
Adeno-Associated Virus (AAV) Type 5 Rep Protein Cleaves a Unique Terminal Resolution Site Compared with Other AAV Serotypes
10.1128/jvi.73.5.4293-4298.1999 · 1999 · External reference
Isolation and genetic characterization of a novel adeno-associated virus from Muscovy ducks in China
10.3382/ps/pex235 · 2017 · External reference
A parvovirus isolated from royal python (Python regius) is a member of the genus Dependovirus
10.1099/vir.0.19616-0 · 2004 · External reference
Molecular characterization of the heparin-dependent transduction domain on the capsid of a novel adeno-associated virus isolate, AAV(VR-942)
10.1128/jvi.00672-08 · 2008 · External reference
Adeno-associated virus type 12 (AAV12): a novel AAV serotype with sialic acid- and heparan sulfate proteoglycan-independent transduction activity
10.1128/jvi.02012-07 · 2008 · External reference
Cloning and characterization of adeno-associated virus type 5
10.1128/jvi.73.2.1309-1319.1999 · 1999 · External reference
Cloning of adeno-associated virus type 4 (AAV4) and generation of recombinant AAV4 particles
10.1128/jvi.71.9.6823-6833.1997 · 1997 · External reference
Novel adeno-associated viruses from rhesus monkeys as vectors for human gene therapy
10.1073/pnas.182412299 · 2002 · External reference
Nucleotide sequence and organization of the adeno-associated virus 2 genome
10.1128/jvi.45.2.555-564.1983 · 1983 · External reference
Gene therapy vectors based on adeno-associated virus type 1
10.1128/jvi.73.5.3994-4003.1999 · 1999 · External reference
Clades of Adeno-associated viruses are widely disseminated in human tissues
10.1128/jvi.78.12.6381-6388.2004 · 2004 · External reference
Long-Term Safety and Efficacy of Factor IX Gene Therapy in Hemophilia B
10.1056/nejmoa1407309 · 2014 · External reference
Roles of adeno-associated virus Rep protein and human chromosome 19 in site-specific recombination
10.1128/jvi.74.9.3953-3966.2000 · 2000 · External reference
A link between severe hepatitis in children and adenovirus 41 and adeno-associated virus 2 infections
10.1099/jgv.0.001783 · 2022 · External reference
The Cellular TATA Binding Protein Is Required for Rep-Dependent Replication of a Minimal Adeno-Associated Virus Type 2 p5 Element
10.1128/jvi.79.17.11082-11094.2005 · 2005 · External reference
Improved Genome Packaging Efficiency of Adeno-associated Virus Vectors Using Rep Hybrids
10.1128/jvi.00773-21 · 2021 · External reference
The AAV2.7m8 capsid packages a higher degree of heterogeneous vector genomes than AAV2
10.1038/s41434-024-00477-7 · 2024 · External reference
circlize implements and enhances circular visualization in R
10.1093/bioinformatics/btu393 · 2014 · External reference
Production of lentiviral vectors using novel, enzymatically produced, linear DNA
10.1038/s41434-018-0056-1 · 2019 · External reference
Understanding capsid assembly and genome packaging for adeno-associated viruses
10.2217/fvl-2017-0011 · 2017 · External reference
DNA helicase-mediated packaging of adeno-associated virus type 2 genomes into preformed capsids
10.1093/emboj/20.12.3282 · 2001 · External reference
Cross-Packaging of a Single Adeno-Associated Virus (AAV) Type 2 Vector Genome into Multiple AAV Serotypes Enables Transduction with Broad Specificity
10.1128/jvi.76.2.791-801.2002 · 2002 · External reference
Adeno-Associated Virus (AAV) Type 5 Rep Protein Cleaves a Unique Terminal Resolution Site Compared with Other AAV Serotypes
10.1128/jvi.73.5.4293-4298.1999 · 1999 · External reference
In vitro resolution of adeno-associated virus DNA hairpin termini by wild-type Rep protein is inhibited by a dominant-negative mutant of rep
10.1128/jvi.66.2.1236-1240.1992 · 1992 · External reference
The Adenoviral E1B-55k Protein Present in HEK293 Cells Mediates Abnormal Accumulation of Key WNT Signaling Proteins in Large Cytoplasmic Aggregates
10.3390/genes12121920 · 2021 · External reference
Analytical characterization of full, intermediate, and empty AAV capsids
10.1038/s41434-024-00444-2 · 2024 · External reference
Teaching an old vector new tricks: the surprising versatility of AAV vaccines
10.1128/jvi.00730-25 · 2025 · External reference
Adeno-associated virus type 2 Rep78 induces apoptosis through caspase activation independently of p53
10.1128/jvi.74.20.9441-9450.2000 · 2000 · External reference
Undetectable Transcription of cap in a Clinical AAV Vector: Implications for Preformed Capsid in Immune Responses
10.1038/mt.2008.227 · 2009 · External reference
A novel class of self-complementary AAV vectors with multiple advantages based on cceAAV lacking mutant ITR
10.1016/j.omtm.2024.101206 · 2024 · External reference
Elimination of contaminating cap genes in AAV vector virions reduces immune responses and improves transgene expression in a canine gene therapy model
10.1038/gt.2014.4 · 2014 · External reference
Immune Responses to AAV in Clinical Trials
10.2174/156652311796150354 · 2011 · External reference
Identification of a myotropic AAV by massively parallel in vivo evaluation of barcoded capsid variants
10.1038/s41467-020-19230-w · 2020 · External reference
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing
10.1038/s41467-022-33745-4 · 2022 · External reference
Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis
10.1038/sj.gt.3301514 · 2001 · External reference
Enhanced transgene expression from single-stranded AAV vectors in human cells in vitro and in murine hepatocytes in vivo
10.1016/j.omtn.2024.102196 · 2024 · External reference
Trimmomatic: a flexible trimmer for Illumina sequence data
10.1093/bioinformatics/btu170 · 2014 · External reference
Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM
2013 · External reference
Twelve years of SAMtools and BCFtools
10.1093/gigascience/giab008 · 2021 · External reference
