Research graph
References from Molecular Programming of CRISPR Diagnostics: From Target Recognition to Field Deployment. Local targets link to admitted publications; unresolved targets remain external evidence.
REASSURED Diagnostics to Inform Disease Control Strategies, Strengthen Health Systems and Improve Patient Outcomes
10.1038/s41564-018-0295-3 · 2019 · External reference
Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care Diagnosis
10.1021/acsnano.0c10035 · 2021 · External reference
Detection of 2019 Novel Coronavirus (2019-nCoV) by Real-Time RT-PCR
10.2807/1560-7917.es.2020.25.3.2000045 · 2020 · External reference
Forty Years of Molecular Diagnostics for Infectious Diseases
10.1128/jcm.02446-21 · 2022 · External reference
Lack of Specificity Associated with Using Molecular Beacons in Loop Mediated Amplification Assays
10.1186/s12896-019-0549-z · 2019 · External reference
Review: A Comprehensive Summary of a Decade Development of the Recombinase Polymerase Amplification
10.1039/c8an01621f · 2019 · External reference
Nucleic Acid Detection with CRISPR-Cas13a/C2c2
10.1126/science.aam9321 · 2017 · External reference
CRISPR-Cas12a Target Binding Unleashes Indiscriminate Single-Stranded DNase Activity
10.1126/science.aar6245 · 2018 · External reference
Streamlined Inactivation, Amplification, and Cas13-Based Detection of SARS-CoV-2
10.1038/s41467-020-19097-x · 2020 · External reference
Ultrasensitive and Visual Detection of SARS-CoV-2 Using All-in-One Dual CRISPR-Cas12a Assay
10.1038/s41467-020-18575-6 · 2020 · External reference
CRISPR-Based Diagnostics
10.1038/s41551-021-00760-7 · 2021 · External reference
CRISPR-Cas-Based Techniques for Pathogen Detection: Retrospect, Recent Advances, and Future Perspectives
10.1016/j.jare.2022.10.011 · 2023 · External reference
CRISPR-Based Precision Molecular Diagnostics for Disease Detection and Surveillance
10.1021/acsabm.3c00439 · 2023 · External reference
CRISPR/Cas and Argonaute-Based Biosensors for Nucleic Acid Detection
10.1016/j.talanta.2025.128210 · 2025 · External reference
Amplification-Free CRISPR/Cas Detection Technology: Challenges, Strategies, and Perspectives
10.1039/d2cs00594h · 2023 · External reference
CRISPR-Based One-Pot Detection: A Game-Changer in Nucleic Acid Analysis
10.1016/j.bios.2025.117786 · 2025 · External reference
CRISPR-Based Non-Nucleic Acid Detection
10.1016/j.tibtech.2025.04.012 · 2025 · External reference
CRISPR/Dx-Based Colorimetric and Electrochemical Detection Systems for POCT Applications
10.1016/j.bios.2025.117778 · 2025 · External reference
CRISPR-Cas Diagnostics (CRISPR-Dx) of Viral Pathogens in Low and Limited Resource Areas: Current State and Future Directions
10.1016/j.trac.2025.118590 · 2026 · External reference
Determinants of CRISPR Cas12a Nuclease Activation by DNA and RNA Targets
10.1093/nar/gkae152 · 2024 · External reference
Multiplexed CRISPR-Based Nucleic Acid Detection Using a Single Cas Protein
10.1021/acs.analchem.3c01861 · 2023 · External reference
Characterization of Cas12a Nucleases Reveals Diverse PAM Profiles between Closely-Related Orthologs
10.1093/nar/gkaa272 · 2020 · External reference
Evolutionary Classification of CRISPR-Cas Systems: A Burst of Class 2 and Derived Variants
10.1038/s41579-019-0299-x · 2020 · External reference
C2c2 Is a Single-Component Programmable RNA-Guided RNA-Targeting CRISPR Effector
10.1126/science.aaf5573 · 2016 · External reference
SHERLOCK: Nucleic Acid Detection with CRISPR Nucleases
10.1038/s41596-019-0210-2 · 2019 · External reference
Designing a Cas9/gRNA-Assisted Quantitative Real-Time PCR (CARP) Assay for Identification of Point Mutations Leading to Rifampicin Resistance in the Human Pathogen Mycobacterium tuberculosis
10.1016/j.gene.2023.147173 · 2023 · External reference
HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation
10.1021/acssynbio.9b00209 · 2019 · External reference
Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems
10.1016/j.molcel.2015.10.008 · 2015 · External reference
Glycerol Additive Boosts 100-Fold Sensitivity Enhancement for One-Pot RPA-CRISPR/Cas12a Assay
10.1021/acs.analchem.2c00616 · 2022 · External reference
Tunable Control of Cas12 Activity Promotes Universal and Fast One-Pot Nucleic Acid Detection
10.1038/s41467-025-56516-3 · 2025 · External reference
Programmed DNA Destruction by Miniature CRISPR-Cas14 Enzymes
10.1126/science.aav4294 · 2018 · External reference
Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity
10.1016/j.cell.2018.10.045 · 2018 · External reference
Unleashing High Trans-Substrate Cleavage Kinetics of Cas12a for Nucleic Acid Diagnostics
10.1093/nar/gkaf712 · 2025 · External reference
Highly Efficient DNA Reporter for CRISPR/Cas12a-Based Specific and Sensitive Biosensor
10.1007/s13206-022-00081-0 · 2022 · External reference
Multiplexed and Portable Nucleic Acid Detection Platform with Cas13, Cas12a, and Csm6
10.1126/science.aaq0179 · 2018 · External reference
Enhancement of CRISPR/Cas12a Trans-Cleavage Activity Using Hairpin DNA Reporters
10.1093/nar/gkac578 · 2022 · External reference
MeCas12a, a Highly Sensitive and Specific System for COVID-19 Detection
10.1002/advs.202001300 · 2020 · External reference
CRISPR-Cas12a Exhibits Metal-Dependent Specificity Switching
10.1093/nar/gkae613 · 2024 · External reference
Harnessing Noncanonical Trans-Cleavage Characteristics of Cas12 and Cas13a to Enhance CRISPR-Based Diagnostics
10.1038/s42003-024-07000-z · 2024 · External reference
Development of a Naked Eye CRISPR-Cas12a and -Cas13a Multiplex Point-of-Care Detection of Genetically Modified Swine
10.1021/acssynbio.3c00089 · 2023 · External reference
Multiplexed CRISPR-Based Microfluidic Platform for Clinical Testing of Respiratory Viruses and Identification of SARS-CoV-2 Variants
10.1038/s41591-022-01734-1 · 2022 · External reference
Iterative crRNA Design and a PAM-Free Strategy Enabled an Ultra-Specific RPA-CRISPR/Cas12a Detection Platform
10.1038/s42003-024-07173-7 · 2024 · External reference
Profiling crRNA Architectures for Enhanced Cas12 Biosensing
10.1038/s42003-025-08356-6 · 2025 · External reference
Split crRNA with CRISPR-Cas12a Enabling Highly Sensitive and Multiplexed Detection of RNA and DNA
10.1038/s41467-024-52691-x · 2024 · External reference
Development of a CRISPR/Cas-Based Detection Platform for Tracking Decreased Susceptibility to Cephalosporins in Neisseria gonorrheae
10.1021/acs.analchem.5c01400 · 2025 · External reference
Enhancement of Trans-Cleavage Activity of Cas12a with Engineered crRNA Enables Amplified Nucleic Acid Detection
10.1038/s41467-020-18615-1 · 2020 · External reference
Sensitive and Visual Detection of SARS-CoV-2 Using RPA-Cas12a One-Step Assay with ssDNA-Modified crRNA
10.1016/j.aca.2024.342693 · 2024 · External reference
Chemical Modifications in RNA Interference and CRISPR/Cas Genome Editing Reagents
10.1007/978-1-0716-0290-4_2 · 2020 · External reference
Gene Editing with CRISPR-Cas12a Guides Possessing Ribose-Modified Pseudoknot Handles
10.1038/s41467-021-26989-z · 2021 · External reference
Identification of a Key Nucleotide Influencing Cas12a crRNA Activity for Universal Photo-Controlled CRISPR Diagnostics
10.1038/s41467-025-62082-5 · 2025 · External reference
Unmodificated Stepless Regulation of CRISPR/Cas12a Multi-Performance
10.1093/nar/gkad748 · 2023 · External reference
Harnessing Multiplex crRNA in the CRISPR/Cas12a System Enables an Amplification-Free DNA Diagnostic Platform for ASFV Detection
10.1021/acs.analchem.2c01588 · 2022 · External reference
Loop-Mediated Isothermal Amplification of DNA
10.1093/nar/28.12.e63 · 2000 · External reference
DNA Detection Using Recombination Proteins
10.1371/journal.pbio.0040204 · 2006 · External reference
Thermally Programmed One-Pot CRISPR Assay for on-Site Pandemic Surveillance
10.1038/s41467-025-65193-1 · 2025 · External reference
SPECIAL: Phosphorothioate dNTP Assisted RPA Equipped with CRISPR/Cas12a Amplifier Enables High-Specific Nucleic Acid Testing
10.1016/j.bios.2025.117421 · 2025 · External reference
Cascade CRISPR/Cas Enables Amplification-Free microRNA Sensing with fM-Sensitivity and Single-Base-Specificity
10.1039/d0cc06412b · 2021 · External reference
A CRISPR-Cas Autocatalysis-Driven Feedback Amplification Network for Supersensitive DNA Diagnostics
10.1126/sciadv.abc7802 · 2021 · External reference
Applications of Catalytic Hairpin Assembly Reaction in Biosensing
10.1002/smll.201902989 · 2019 · External reference
An Ultralocalized Cas13a Assay Enables Universal and Nucleic Acid Amplification-Free Single-Molecule RNA Diagnostics
10.1021/acsnano.0c08165 · 2021 · External reference
Integration of Logic Gates to CRISPR/Cas12a System for Rapid and Sensitive Detection of Pathogenic Bacterial Genes
10.1016/j.aca.2020.05.017 · 2020 · External reference
Dual Mature microRNA-Responsive Logic Biosensing Platform Based on CRISPR/Cas12a and DNA Nanocage
10.1016/j.talanta.2024.127078 · 2025 · External reference
Enhanced the Trans-Cleavage Activity of CRISPR-Cas12a Using Metal-Organic Frameworks as Stimulants for Efficient Electrochemical Sensing of Circulating Tumor DNA
10.1002/advs.202417206 · 2025 · External reference
Quencher-Free CRISPR-Based Molecular Detection Using an Amphiphilic DNA Fluorescence Probe
10.1016/j.bios.2024.117054 · 2025 · External reference
SERS-Based CRISPR/Cas Assay on Microfluidic Paper Analytical Devices for Supersensitive Detection of Pathogenic Bacteria in Foods
10.1016/j.bios.2022.114167 · 2022 · External reference
G-Quadruplex Molecular Beacon: A Versatile CRISPR/Cas12a Reporter for Rapid and Label-Free Biosensing
10.1016/j.snb.2023.133310 · 2023 · External reference
Universal Smartphone-Assisted Label-Free CRISPR/Cas12a-DNAzyme Chemiluminescence Biosensing Platform for on-Site Detection of Nucleic Acid and Non-Nucleic Acid Targets
10.1016/j.bios.2023.115929 · 2024 · External reference
Programmable RNA Detection with CRISPR-Cas12a
10.1038/s41467-023-41006-1 · 2023 · External reference
“RESET” Effect: Random Extending Sequences Enhance the Trans-Cleavage Activity of CRISPR/Cas12a
10.1021/acs.analchem.2c01401 · 2022 · External reference
Comprehensive Approaches to Expanding CRISPR Biosensor Applications beyond Nucleic Acid Targets
10.1016/j.trac.2025.118179 · 2025 · External reference
Split Aptamer Regulated CRISPR/Cas12a Biosensor for 17β-Estradiol through a Gap-Enhanced Raman Tags Based Lateral Flow Strategy
10.1016/j.bios.2022.114548 · 2022 · External reference
“Turn-on” Aptamer-Immune Lateral Flow Assays for the Detection of Small Molecule Targets Based on CHA-Assisted and CRISPR/Cas12a Mediated Signal Transduction and Amplification
10.1016/j.bios.2025.117593 · 2025 · External reference
Smartphone-Based Free-to-Total Prostate Specific Antigen Ratio Detection System Using a Colorimetric Reaction Integrated with Proximity-Induced Bio-Barcode and CRISPR/Cas12a Assay
10.1002/smll.202310212 · 2024 · External reference
Ultrasensitive Clinical Identification of Hepatitis B Surface Antigen (HBsAg) by CRISPR-Assisted Nanopore Sensing
10.1016/j.bios.2025.117579 · 2025 · External reference
Miniaturized Surface Engineered Technologies for Multiplex Biosensing Devices
10.1002/elan.202200355 · 2023 · External reference
Cas-OFFinder: A Fast and Versatile Algorithm That Searches for Potential off-Target Sites of Cas9 RNA-Guided Endonucleases
10.1093/bioinformatics/btu048 · 2014 · External reference
CHOPCHOP v3: Expanding the CRISPR Web Toolbox beyond Genome Editing
10.1093/nar/gkz365 · 2019 · External reference
CRISPOR: Intuitive Guide Selection for CRISPR/Cas9 Genome Editing Experiments and Screens
10.1093/nar/gky354 · 2018 · External reference
CrispHunter-Enabled Systematic crRNA Design and Structure-Optimized Hairpin Probes for Enhanced CRISPR-Cas12a Detection of Burkholderia pseudomallei
10.1016/j.bios.2025.117912 · 2025 · External reference
PathoGD: An Integrative Genomics Approach to Primer and Guide RNA Design for CRISPR-Based Diagnostics
10.1038/s42003-025-07591-1 · 2025 · External reference
Krisp: A Python Package to Aid in the Design of CRISPR and Amplification-Based Diagnostic Assays from Whole Genome Sequencing Data
10.1371/journal.pcbi.1012139 · 2024 · External reference
CaSilico: A Versatile CRISPR Package for in Silico CRISPR RNA Designing for Cas12, Cas13, and Cas14
10.3389/fbioe.2022.957131 · 2022 · External reference
Optimized sgRNA Design to Maximize Activity and Minimize Off-Target Effects of CRISPR-Cas9
10.1038/nbt.3437 · 2016 · External reference
DeepCRISPR: Optimized CRISPR Guide RNA Design by Deep Learning
10.1186/s13059-018-1459-4 · 2018 · External reference
Deep Learning Enhancing Guide RNA Design for CRISPR/Cas12a-Based Diagnostics
10.1002/imt2.214 · 2024 · External reference
Designing Sensitive Viral Diagnostics with Machine Learning
10.1038/s41587-022-01213-5 · 2022 · External reference
CRISPR-GPT for Agentic Automation of Gene-Editing Experiments
10.1038/s41551-025-01463-z · 2026 · External reference
Highly Specific and Sensitive Detection of Burkholderia pseudomallei Genomic DNA by CRISPR-Cas12a
10.1371/journal.pntd.0010659 · 2022 · External reference
Benchmarking CRISPR-BP34 for Point-of-Care Melioidosis Detection in Low-Income and Middle-Income Countries: A Molecular Diagnostics Study
10.1016/s2666-5247(23)00378-6 · 2024 · External reference
Amplification-Free CRISPR/Cas12a Biosensor Integrating AuNPs-Mediated Surface Plasmon Resonance for Human Papillomavirus Detection and Genotyping
10.1016/j.bios.2025.117960 · 2025 · External reference
Enhanced One-Pot Dual-CRISPR-Based Assay Lyophilized on a 3D-Printed Disc for Field-Deployable Multiplex Bacteria Detection
10.1002/advs.202509355 · 2025 · External reference
Field-Deployable Viral Diagnostics Using CRISPR-Cas13
10.1126/science.aas8836 · 2018 · External reference
FLASH: A next-Generation CRISPR Diagnostic for Multiplexed Detection of Antimicrobial Resistance Sequences
10.1093/nar/gkz418 · 2019 · External reference
FLASH-TB: An Application of Next-Generation CRISPR to Detect Drug Resistant Tuberculosis from Direct Sputum
10.1128/jcm.01634-22 · 2023 · External reference
A Streamlined CRISPR-Based Test for Tuberculosis Detection Directly from Sputum
10.1126/sciadv.adx2067 · 2025 · External reference
A Smartphone-Read Ultrasensitive and Quantitative Saliva Test for COVID-19
10.1126/sciadv.abe3703 · 2021 · External reference
Carrying out Pseudo Dual Nucleic Acid Detection from Sample to Visual Result in a Polypropylene Bag with CRISPR/Cas12a
10.1016/j.bios.2021.113001 · 2021 · External reference
Simplified Cas13-Based Assays for the Fast Identification of SARS-CoV-2 and Its Variants
10.1038/s41551-022-00889-z · 2022 · External reference
Deep Learning-Enhanced Hand-Driven Spatial Encoding Microfluidics for Multiplexed Molecular Testing at Home
10.1021/acsnano.5c04309 · 2025 · External reference
Miniaturized Single-Step Duplex CRISPR Diagnostic Platform for at-Home Molecular Testing of HPV16 and HPV18
10.1021/acssensors.5c03020 · 2026 · External reference
