Research graph
References from Effect of target gene sequence evenness and dominance on real-time PCR quantification of artificial sulfate-reducing microbial communities. Local targets link to admitted publications; unresolved targets remain external evidence.
Simple Absolute Quantification Method Correcting for Quantitative PCR Efficiency Variations for Microbial Community Samples
10.1128/aem.07878-11 · 2012 · External reference
The degenerate primer design problem: Theory and applications
10.1089/cmb.2005.12.431 · 2005 · External reference
The Effect of Primer-Template Mismatches on the Detection and Quantification of Nucleic Acids Using the 5 ’ Nuclease Assay
10.2353/jmoldx.2010.090035 · 2010 · External reference
Bias in template-to-product ratios in multitemplate PCR
10.1128/aem.64.10.3724-3730.1998 · 1998 · External reference
Bias and artifacts in multitemplate polymerase chain reactions (PCR)
10.1016/s1389-1723(03)90130-7 · 2003 · External reference
The Use of Degenerate Primers in qPCR Analysis of Functional Genes Can Cause Dramatic Quantification Bias as Revealed by Investigation of nifH Primer Performance
10.1007/s00248-017-0968-0 · 2017 · External reference
PCR-Induced Sequence Artifacts and Bias: Insights from Comparison of Two 16S rRNA Clone Libraries Constructed from the Same Sample
10.1128/aem.71.12.8966-8969.2005 · 2005 · External reference
Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR
10.1128/aem.67.8.3753-3755.2001 · 2001 · External reference
Effect of PCR template concentration on the composition and distribution of total community 16S rDNA clone libraries
10.1046/j.1365-294x.1997.00205.x · 1997 · External reference
Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis
2006 · External reference
Controlling for Contaminants in Low-Biomass 16S rRNA Gene Sequencing Experiments
10.1128/msystems.00290-19 · 2019 · External reference
Contamination in Low Microbial Biomass Microbiome Studies: Issues and Recommendations
10.1016/j.tim.2018.11.003 · 2019 · External reference
Experimental factors affecting PCR-based estimates of microbial species richness and evenness
10.1038/ismej.2009.153 · 2010 · External reference
Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species
10.1128/aem.61.7.2798-2801.1995 · 1995 · External reference
Development and comparison of SYBR Green quantitative real-time PCR assays for detection and enumeration of sulfate-reducing bacteria in stored swine manure
10.1111/j.1365-2672.2008.03900.x · 2008 · External reference
Rapid detection and quantification of bisulfite reductase genes in oil field samples using real-time PCR
10.1111/j.1574-6941.2009.00714.x · 2009 · External reference
Phylogenetic and environmental diversity of DsrAB-type dissimilatory (bi) sulfite reductases
10.1038/ismej.2014.208 · 2015 · External reference
A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition
10.1186/1471-2105-9-326 · 2008 · External reference
Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data
10.1093/bioinformatics/bts199 · 2012 · External reference
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Assessment and modification of degenerate qPCR primers that amplify functional genes from etheneotrophs and vinyl chloride-assimilators
10.1111/j.1472-765x.2011.03144.x · 2011 · External reference
An improved protocol with a highly degenerate primer targeting copper-containing membrane-bound monooxygenase genes for community analysis of methane- and ammonia-oxidizing bacteria
2017 · External reference
Abundances and potential activities of nitrogen cycling microbial communities along a chronosequence of a glacier forefield
10.1038/ismej.2010.184 · 2011 · External reference
Abundance of narG, nirS, nirK, and nosZ genes of denitrifying bacteria during primary successions of a glacier foreland
10.1128/aem.00439-06 · 2006 · External reference