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References from Next-generation sequencing in the etiological diagnosis of severe childhood pneumonia. Local targets link to admitted publications; unresolved targets remain external evidence.
Global incidence, prevalence, years lived with disability (YLDs), disability-adjusted life-years (DALYs), and healthy life expectancy (HALE) for 371 diseases and injuries in 204 countries and territories and 811 subnational locations, 1990–2021: a systematic analysis for the global burden of disease study 2021
10.1016/s0140-6736(24)00757-8 · 2024 · External reference
Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000
10.1016/s0140-6736(12)60560-1 · 2012 · External reference
Metagenomic next-generation sequencing to detect pneumocystis jirovecii pneumonia in critically ill, HIV-negative children: a retrospective multicenter study
10.1186/s12890-026-04163-9 · 2026 · External reference
10.3760/cma.j.cn112140-20240728-00523
10.3760/cma.j.cn112140-20240728-00523 · 2024 · External reference
Incidence of community-acquired pneumonia in urban China: a national population-based study
10.1016/j.vaccine.2020.11.004 · 2020 · External reference
mNGS in clinical microbiology laboratories: on the road to maturity
10.1080/1040841x.2019.1681933 · 2019 · External reference
The application status of sequencing technology in global respiratory infectious disease diagnosis
10.1007/s15010-024-02360-4 · 2024 · External reference
Clinical application of metagenomic next-generation sequencing in the diagnosis of severe pneumonia pathogens
10.3389/fcimb.2025.1661213 · 2025 · External reference
Microbiological diagnostic performance of metagenomic next-generation sequencing when applied to clinical practice
10.1093/cid/ciy693 · 2018 · External reference
The diagnostic efficacy of metagenomic next-generation sequencing (mNGS) in pathogen identification of pediatric pneumonia using bronchoalveolar lavage fluid (BALF): a systematic review and meta-analysis
10.1016/j.micpath.2025.107492 · 2025 · External reference
The clinical significance of metagenomic next-generation sequencing of bronchoalveolar lavage fluid in adult patients with severe pneumonia
10.14164/j.cnki.cn11-5581/r.2025.11.021 · 2025 · External reference
Clinical utility of bronchoalveolar lavage fluid metagenomic next-generation sequencing in the etiological diagnosis of community-acquired pneumonia in children
10.1016/j.ijmm.2026.151701 · 2026 · External reference
Evaluation of mortality risk factors in pediatric patients with severe pneumonia within the department of critical care medicine and the prognostic significance of the modified PIRO score for mortality risk
10.3760/cma.j.cn101070-20240118-00038 · 2024 · External reference
Improving pulmonary infection diagnosis with metagenomic next-generation sequencing of bronchoalveolar lavage fluid
10.1099/jmm.0.001808 · 2024 · External reference
Antimicrobial armageddon: the professional guide to conquering antibiotic resistance
10.1007/s12602-026-10928-9 · 2026 · External reference
Detection of pathogens and epidemiological characteristics of community-acquired pneumonia in children using metagenomic next-generation sequencing
10.3855/jidc.20668 · 2025 · External reference
A COVID-19 patient with seven consecutive false-negative rRT-PCR results from sputum specimens
10.1007/s11739-020-02423-y · 2020 · External reference
Detecting respiratory infection in children with cystic fibrosis: cough swab, sputum induction or bronchoalveolar lavage
10.1016/j.prrv.2019.03.001 · 2019 · External reference
Metagenomic and targeted next-generation sequencing in infectious disease diagnostics: current applications, challenges, and future perspectives
10.3390/diagnostics16070991 · 2026 · External reference
Bacterial multiplex polymerase chain reaction tests for the diagnosis and management of pneumonia: ready for prime time?
10.1136/thorax-2024-222297 · 2025 · External reference
The value of metagenomic next-generation sequencing in lower respiratory tract infections among critically ill patients in the ICU
10.3389/fcimb.2026.1746117 · 2026 · External reference
The clinical value of metagenomic next-generation sequencing in severe infections in children
10.7499/j.issn.1008-8830.2110003 · 2022 · External reference
Diagnostic value of alveolar lavage fluid metagenomic sequencing in children with severe mixed Mycoplasma Pneumoniae and pneumococcal pneumonia
10.3969/j.issn.1000-3606.2020.12.003 · 2020 · External reference
High utility of bronchoalveolar lavage fluid metagenomic next-generation sequencing approach for etiological diagnosis of pneumonia
10.1186/s12879-024-10108-6 · 2024 · External reference
Clinical metagenomic next-generation sequencing for pathogen detection
10.1146/annurev-pathmechdis-012418-012751 · 2019 · External reference
Application of metagenomic next-generation sequencing (mNGS) using bronchoalveolar lavage fluid (BALF) in diagnosing pneumonia of children
10.1128/spectrum.01488-22 · 2022 · External reference
[Application of metagenomic next-generation sequencing of bronchoalveolar lavage fluid in the diagnosis and treatment of refractory pneumonia in children]
10.7499/j.issn.1008-8830.2307106 · 2023 · External reference
Utilizing metagenomic next-generation sequencing for diagnosis and lung microbiome probing of pediatric pneumonia through bronchoalveolar lavage fluid in pediatric intensive care unit: results from a large real-world cohort
10.3389/fcimb.2023.1200806 · 2023 · External reference
Clinical evaluation of cell-free and cellular metagenomic next-generation sequencing of infected body fluids
10.1016/j.jare.2023.02.018 · 2024 · External reference
Clinical utility of metagenomic next-generation sequencing in pathogen detection for lower respiratory tract infections
10.1038/s41598-025-03564-w · 2025 · External reference
Comprehensive analysis of microorganisms in severe septic patients in the intensive care unit by mNGS and microbial culture
10.1186/s12866-026-04730-4 · 2026 · External reference
Metagenomic next-generation sequencing for mixed pulmonary infection diagnosis
10.1186/s12890-019-1022-4 · 2019 · External reference
Metagenomic next-generation sequencing contributes to the diagnosis of mixed pulmonary infection: a case report
10.1186/s12941-022-00545-z · 2022 · External reference
Diagnostic value of bronchoalveolar lavage fluid metagenomic next-generation sequencing in pediatric pneumonia
10.3389/fcimb.2022.950531 · 2022 · External reference
Clinical diagnostic application of metagenomic next-generation sequencing in children with severe nonresponding pneumonia
10.1371/journal.pone.0232610 · 2020 · External reference
Diagnosis of mixed infection and a primary immunodeficiency disease using next-generation sequencing: a case report
10.3389/fcimb.2023.1179090 · 2023 · External reference
Blood and bronchoalveolar lavage fluid metagenomic next-generation sequencing in pneumonia
10.1155/2020/6839103 · 2020 · External reference
Application of metagenomic next-generation sequencing in the etiological diagnosis of refractory pneumonia in children
10.3389/fmicb.2024.1357372 · 2024 · External reference
Comparison of targeted next-generation sequencing and metagenomic next-generation sequencing in the identification of pathogens in pneumonia after congenital heart surgery: a comparative diagnostic accuracy study
10.1186/s13052-024-01749-z · 2024 · External reference
Detection of cytomegalovirus in the lower respiratory tract among patients with critical illness: uncovering enhanced potential benefits
10.1186/s13054-025-05456-x · 2025 · External reference
Application of metagenomic next-generation sequencing and targeted metagenomic next-generation sequencing in diagnosing pulmonary infections in immunocompetent and immunocompromised patients
10.3389/fcimb.2024.1439472 · 2024 · External reference
Application of metagenomic next-generation sequencing in the diagnosis of pulmonary invasive fungal disease
10.3389/fcimb.2022.949505 · 2022 · External reference
Clinical utility of metagenomic next-generation sequencing in the diagnosis of invasive pulmonary aspergillosis in acute exacerbation of chronic obstructive pulmonary disease patients in the intensive care unit
10.3389/fcimb.2024.1397733 · 2024 · External reference
Functional metagenomics highlights varied infection states with dynamics of pathogens and antibiotic resistance in lower respiratory tract infections
10.1016/j.heliyon.2024.e38380 · 2024 · External reference
The diagnostic value of bronchoalveolar lavage fluid metagenomic next-generation sequencing in critically ill patients with respiratory tract infections
10.1128/spectrum.00458-24 · 2024 · External reference
Metagenomics next-generation sequencing tests take the stage in the diagnosis of lower respiratory tract infections
10.1016/j.jare.2021.09.012 · 2021 · External reference
The diagnostic value of RNA-mNGS and DNA-mNGS in differentiating bacterial infection from colonization in the lower respiratory tract
10.3389/fcimb.2025.1639148 · 2025 · External reference
Application of next-generation sequencing in the detection of antimicrobial resistance
10.1016/j.cca.2025.120520 · 2026 · External reference
Performance of targeted next-generation sequencing in the detection of respiratory pathogens and antimicrobial resistance genes for children
10.1099/jmm.0.001771 · 2023 · External reference
Exploring drug resistance genes in Acinetobacter baumannii using metagenomic next-generation sequencing
10.3389/fmicb.2025.1669208 · 2025 · External reference
Optimization of early antimicrobial strategies for lung transplant recipients based on metagenomic next-generation sequencing
10.3389/fmicb.2022.839698 · 2022 · External reference
Application of second-generation sequencing in cerebrospinal fluid metagenomics for the diagnosis and treatment of intracranial infections
10.3969/j.issn.1672-6731.2024.06.004 · 2024 · External reference
Diagnostic value of metagenomic next-generation sequencing using bronchoalveolar lavage fluid samples for pathogen detection in children with severe or refractory pneumonia
10.1128/spectrum.01087-24 · 2025 · External reference
Pathogen landscape and synergistic interactions in pediatric ARIs: implications for broad-spectrum surveillance via targeted NGS
10.1128/spectrum.02275-25 · 2026 · External reference
Metagenomic next-generation sequencing on treatment strategies and prognosis of patients with lower respiratory tract infections: a systematic review and meta-analysis
10.1016/j.ijantimicag.2024.107440 · 2025 · External reference
Metagenomic next-generation sequencing in the diagnosis of infectious fever during myelosuppression among pediatric patients with hematological and neoplastic diseases
10.2147/idr.s379582 · 2022 · External reference
Metagenomic next-generation sequencing in infectious diseases: clinical applications, translational challenges, and future directions
10.3390/diagnostics15161991 · 2025 · External reference
Enhancing the detection sensitivity of mNGS in bronchoalveolar lavage fluid through cell counting: an empirical study
10.1016/j.cca.2025.120311 · 2025 · External reference
Clinical applications of metagenomics next-generation sequencing in infectious diseases
10.1631/jzus.b2300029 · 2024 · External reference
The performance of nanopore sequencing in rapid detection of pathogens and antimicrobial resistance genes in blood cultures
10.1016/j.diagmicrobio.2025.116720 · 2025 · External reference
Adaptation of custom capture sequencing panels to the Oxford Nanopore MinION platform
10.1007/s11033-026-11589-1 · 2026 · External reference
A microreactor system for point-of-care viral genome sequencing
10.1016/j.bios.2026.118369 · 2026 · External reference
Clinical bacteriology in low-resource settings: today’s solutions
10.1016/s1473-3099(18)30093-8 · 2018 · External reference
Metagenomics and artificial intelligence in the context of human health
10.1016/j.meegid.2022.105267 · 2022 · External reference
Discovery and cultivation of prokaryotic taxa in the age of metagenomics and artificial intelligence
10.1093/ismejo/wrag012 · 2025 · External reference
Artificial intelligence for the optimal management of community-acquired pneumonia
10.1097/mcp.0000000000001055 · 2024 · External reference
Artificial intelligence and pneumonia: a rapidly evolving frontier
10.1016/s2214-109x(23)00463-1 · 2023 · External reference