References

Redox-Dependent Binding and Conformational Equilibria Govern the Fluorescence Decay of NAD(P)H in Living Cells

10.1002/1873-3468.70125 · ExternalCitation · doi-reference

An Activatable NIR Fluorescent Probe for NAD(P)H and Its Application to the Real-Time Monitoring of p53 Abnormalities In Vivo

10.1002/anie.202301518 · ExternalCitation · doi-reference

Measurement of NAD(P)H and NADPH-Generating Enzymes

10.1007/978-1-0716-2469-2_7 · ExternalCitation · doi-reference

Experimental Design and Reporting Standards for Metabolomics Studies of Mammalian Cell Lines

10.1007/s00018-017-2582-1 · ExternalCitation · doi-reference

Evaluating Mitochondrial Membrane Potential in Cells

10.1007/s10540-007-9033-4 · ExternalCitation · doi-reference

NAD(P)H-Triggered Probe for Dual-Modal Imaging during Energy Metabolism and Novel Strategy of Enhanced Photothermal Therapy in Tumor

10.1016/j.biomaterials.2021.120736 · ExternalCitation · doi-reference

NAD(P)H Binding Configurations Revealed by Time-Resolved Fluorescence and Two-Photon Absorption

10.1016/j.bpj.2023.02.014 · ExternalCitation · doi-reference

Glutathione and Thioredoxin Antioxidant Pathways Synergize to Drive Cancer Initiation and Progression

10.1016/j.ccell.2014.11.019 · ExternalCitation · doi-reference

A Two-Photon Fluorescent Probe Sensitive to NADPH Reveals Metabolic Aberrations in Brain Cells Arising from Mitochondrial Dysfunction in Alzheimer’s Disease

10.1016/j.cej.2024.153652 · ExternalCitation · doi-reference

Succinate Dehydrogenase Supports Metabolic Repurposing of Mitochondria to Drive Inflammatory Macrophages

10.1016/j.cell.2016.08.064 · ExternalCitation · doi-reference

Understanding the Intersections between Metabolism and Cancer Biology

10.1016/j.cell.2016.12.039 · ExternalCitation · doi-reference

Metabolic Instruction of Immunity

10.1016/j.cell.2017.04.004 · ExternalCitation · doi-reference

Metabolomics and Isotope Tracing

10.1016/j.cell.2018.03.055 · ExternalCitation · doi-reference

A Roadmap for Interpreting (13)C Metabolite Labeling Patterns from Cells

10.1016/j.copbio.2015.02.003 · ExternalCitation · doi-reference

Measuring Reactive Oxygen and Nitrogen Species with Fluorescent Probes: Challenges and Limitations

10.1016/j.freeradbiomed.2011.09.030 · ExternalCitation · doi-reference

Small-Molecule Luminescent Probes for the Detection of Cellular Oxidizing and Nitrating Species

10.1016/j.freeradbiomed.2018.03.032 · ExternalCitation · doi-reference

Homeostatic Regulation of NAD(H) and NADP(H) in Cells

10.1016/j.gendis.2023.101146 · ExternalCitation · doi-reference

Redox Metabolism: ROS as Specific Molecular Regulators of Cell Signaling and Function

10.1016/j.molcel.2021.08.018 · ExternalCitation · doi-reference

Mitostasis in Neurons: Maintaining Mitochondria in an Extended Cellular Architecture

10.1016/j.neuron.2017.09.055 · ExternalCitation · doi-reference

In Cancer, All Roads Lead to NADPH

10.1016/j.pharmthera.2021.107864 · ExternalCitation · doi-reference

Real-Time Monitoring of Stromal NADPH Levels in Arabidopsis Using a Metagenome-Derived NADPH-Binding Fluorescent Protein

10.1016/j.plaphy.2024.109260 · ExternalCitation · doi-reference

Redox Homeostasis: The Golden Mean of Healthy Living

10.1016/j.redox.2016.01.010 · ExternalCitation · doi-reference

A Quinoline-Based Indicator for NAD(P)H Multimodal Detection in Vitro and in Vivo: Spectrophotometry and Visible near-Infrared Dual-Channel Lighting-up Fluorescence Imaging

10.1016/j.snb.2022.132694 · ExternalCitation · doi-reference

Near-Infrared Absorption and Emission Probes with Optimal Connection Bridges for Live Monitoring of NAD(P)H Dynamics in Living Systems

10.1016/j.snb.2023.135073 · ExternalCitation · doi-reference

Detection of NAD(P)H by Fluorescent Probe to Reveal the Protective Effects of Natural Antioxidants on Acute Liver Injury

10.1016/j.snb.2024.135618 · ExternalCitation · doi-reference

A Quinolinium-Functionalized Hemicyanine Dye for Ratiometric NAD(P)H Sensing in Live Cells, Kidney Tissues, and Drosophila melanogaster

10.1016/j.snb.2025.138043 · ExternalCitation · doi-reference

Rapid Urine Screening and Staging of Chronic Kidney Disease via NAD(P)H-Activated Dual-Salt Fluorescent Probe

10.1016/j.talanta.2026.129470 · ExternalCitation · doi-reference

The Nrf2 Regulatory Network Provides an Interface between Redox and Intermediary Metabolism

10.1016/j.tibs.2014.02.002 · ExternalCitation · doi-reference

Monitoring Subcellular NADP Redox State with NAPstar Biosensors

10.1016/j.tibs.2025.03.013 · ExternalCitation · doi-reference

Measurement of Mitochondrial Membrane Potential Using Fluorescent Rhodamine Derivatives

10.1016/s0006-3495(99)77214-0 · ExternalCitation · doi-reference

Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks

10.1021/acs.chemrev.8b00333 · ExternalCitation · doi-reference

Highly Sensitive Cyanine Dyes for Rapid Sensing of NAD(P)H in Mitochondria and First-Instar Larvae of Drosophila melanogaster

10.1021/acsabm.3c00320 · ExternalCitation · doi-reference

Syntheses and Applications of Coumarin-Derived Fluorescent Probes for Real-Time Monitoring of NAD(P)H Dynamics in Living Cells across Diverse Chemical Environments

10.1021/acsabm.4c00595 · ExternalCitation · doi-reference

Mitochondrial Targetable Turn-On Fluorescent Probe for Fast Detection of NAD(P)H in Living Cells and In Vivo

10.1021/acsabm.4c00755 · ExternalCitation · doi-reference

Deep-Red and Near-Infrared Compact Cyanine Dyes for Sensitive NAD(P)H Sensing in Live Cells and Kidney Disease Tissues

10.1021/acsabm.4c01345 · ExternalCitation · doi-reference

A Rhodamine-Based Ratiometric Fluorescent Sensor for Dual-Channel Visible and Near-Infrared Emission Detection of NAD(P)H in Living Cells and Fruit Fly Larvae

10.1021/acsabm.4c01912 · ExternalCitation · doi-reference

Deep-Red Cyanine-Based Fluorescent Probes with 6-Quinolinium Acceptors for Mitochondrial NAD(P)H Imaging in Live Cells and Human Diseased Kidney Tissues

10.1021/acsabm.5c00015 · ExternalCitation · doi-reference

A Mitochondria-Targeted Fluorescent Probe for Monitoring NADPH Overproduction during Influenza Virus Infection

10.1021/acssensors.2c02458 · ExternalCitation · doi-reference

NAD(P)H Activated Fluorescent Probe for Rapid Intraoperative Pathological Diagnosis and Tumor Histological Grading

10.1021/cbmi.3c00076 · ExternalCitation · doi-reference

Fluorogenic Rhodamine-Based Chemigenetic Biosensor for Monitoring Cellular NADPH Dynamics

10.1021/jacs.3c13137 · ExternalCitation · doi-reference

Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets

10.1021/jacs.4c11804 · ExternalCitation · doi-reference

Drug Development: Raise Standards for Preclinical Cancer Research

10.1038/483531a · ExternalCitation · doi-reference

Mitochondrial Dysfunction and Oxidative Stress in Neurodegenerative Diseases

10.1038/nature05292 · ExternalCitation · doi-reference

A Call for Transparent Reporting to Optimize the Predictive Value of Preclinical Research

10.1038/nature11556 · ExternalCitation · doi-reference

Reductive Carboxylation Supports Redox Homeostasis during Anchorage-Independent Growth

10.1038/nature17393 · ExternalCitation · doi-reference

Reaction-Based Small-Molecule Fluorescent Probes for Chemoselective Bioimaging

10.1038/nchem.1500 · ExternalCitation · doi-reference

Separating NADH and NADPH Fluorescence in Live Cells and Tissues Using FLIM

10.1038/ncomms4936 · ExternalCitation · doi-reference

Going Deeper than Microscopy: The Optical Imaging Frontier in Biology

10.1038/nmeth.1483 · ExternalCitation · doi-reference

Fiji: An Open-Source Platform for Biological-Image Analysis

10.1038/nmeth.2019 · ExternalCitation · doi-reference

Apollo-NADP+: A Spectrally Tunable Family of Genetically Encoded Sensors for NADP+

10.1038/nmeth.3764 · ExternalCitation · doi-reference

Genetically Encoded Fluorescent Sensors Reveal Dynamic Regulation of NADPH Metabolism

10.1038/nmeth.4306 · ExternalCitation · doi-reference

Assessing Phototoxicity in Live Fluorescence Imaging

10.1038/nmeth.4344 · ExternalCitation · doi-reference

Data-Analysis Strategies for Image-Based Cell Profiling

10.1038/nmeth.4397 · ExternalCitation · doi-reference

Cell Painting, a High-Content Image-Based Assay for Morphological Profiling Using Multiplexed Fluorescent Dyes

10.1038/nprot.2016.105 · ExternalCitation · doi-reference

A Guide to Immunometabolism for Immunologists

10.1038/nri.2016.70 · ExternalCitation · doi-reference

Beyond Oxidative Stress: An Immunologist’s Guide to Reactive Oxygen Species

10.1038/nri3423 · ExternalCitation · doi-reference

ROS as Signalling Molecules: Mechanisms That Generate Specificity in ROS Homeostasis

10.1038/nrm2256 · ExternalCitation · doi-reference

Cellular Mechanisms and Physiological Consequences of Redox-Dependent Signalling

10.1038/nrm3801 · ExternalCitation · doi-reference

A Guide to (13)C Metabolic Flux Analysis for the Cancer Biologist

10.1038/s12276-018-0060-y · ExternalCitation · doi-reference

Multi-Photon, Label-Free Photoacoustic and Optical Imaging of NADH in Brain Cells

10.1038/s41377-025-01895-x · ExternalCitation · doi-reference

NADPH Homeostasis in Cancer: Functions, Mechanisms and Therapeutic Implications

10.1038/s41392-020-00326-0 · ExternalCitation · doi-reference

Redox Regulation: Mechanisms, Biology and Therapeutic Targets in Diseases

10.1038/s41392-024-02095-6 · ExternalCitation · doi-reference

NADPH Oxidase Family Proteins: Signaling Dynamics to Disease Management

10.1038/s41423-022-00858-1 · ExternalCitation · doi-reference

Use Cases, Best Practice and Reporting Standards for Metabolomics in Regulatory Toxicology

10.1038/s41467-019-10900-y · ExternalCitation · doi-reference

NERNST: A Genetically-Encoded Ratiometric Non-Destructive Sensing Tool to Estimate NADP(H) Redox Status in Bacterial, Plant and Animal Systems

10.1038/s41467-023-38739-4 · ExternalCitation · doi-reference

Glucose-6-Phosphate Dehydrogenase Maintains Redox Homeostasis and Biosynthesis in LKB1-Deficient KRAS-Driven Lung Cancer

10.1038/s41467-024-50157-8 · ExternalCitation · doi-reference

A Family of NADPH/NADP(+) Biosensors Reveals in Vivo Dynamics of Central Redox Metabolism across Eukaryotes

10.1038/s41467-024-55302-x · ExternalCitation · doi-reference

Mitochondrial NADPH Fuels Mitochondrial Fatty Acid Synthesis and Lipoylation to Power Oxidative Metabolism

10.1038/s41556-025-01655-4 · ExternalCitation · doi-reference

Label-Free Biomedical Optical Imaging

10.1038/s41566-023-01299-6 · ExternalCitation · doi-reference

Reactive Oxygen Species (ROS) as Pleiotropic Physiological Signalling Agents

10.1038/s41580-020-0230-3 · ExternalCitation · doi-reference

Structure of Human Phagocyte NADPH Oxidase in the Activated State

10.1038/s41586-024-07056-1 · ExternalCitation · doi-reference

Cytosolic and Mitochondrial NADPH Fluxes Are Independently Regulated

10.1038/s41589-023-01283-9 · ExternalCitation · doi-reference

Towards Community-Driven Metadata Standards for Light Microscopy: Tiered Specifications Extending the OME Model

10.1038/s41592-021-01327-9 · ExternalCitation · doi-reference

Caveat Fluorophore: An Insiders’ Guide to Small-Molecule Fluorescent Labels

10.1038/s41592-021-01338-6 · ExternalCitation · doi-reference

Community-Developed Checklists for Publishing Images and Image Analyses

10.1038/s41592-023-01987-9 · ExternalCitation · doi-reference

Metabolic Trajectory of Cellular Differentiation in Small Intestine by Phasor Fluorescence Lifetime Microscopy of NADH

10.1038/srep00568 · ExternalCitation · doi-reference

A Visible and Near-Infrared Dual-Fluorescent Probe for Discrimination between Cys/Hcy and GSH and Its Application in Bioimaging

10.1039/d0bm01237h · ExternalCitation · doi-reference

Photoinduced Electron Transfer (PeT) Based Fluorescent Probes for Cellular Imaging and Disease Therapy

10.1039/d1cs01097b · ExternalCitation · doi-reference

Rapid Quantitative Assays for Glucose-6-Phosphate Dehydrogenase (G6PD) and Hemoglobin Combined on a Capillary-Driven Microfluidic Chip

10.1039/d1lc00354b · ExternalCitation · doi-reference

An NIR Fluorescent/Photoacoustic Dual-Mode Probe of NADPH for Tumor Imaging

10.1039/d2cc06354a · ExternalCitation · doi-reference

Twisted Intramolecular Charge Transfer (TICT) Based Fluorescent Probes and Imaging Agents

10.1039/d3cs01118f · ExternalCitation · doi-reference

Non-Invasive Diagnosis of Bacterial and Non-Bacterial Inflammations Using a Dual-Enzyme-Responsive Fluorescent Indicator

10.1039/d3sc06866h · ExternalCitation · doi-reference

Sensitive Monitoring of NAD(P)H Levels within Cancer Cells Using Mitochondria-Targeted near-Infrared Cyanine Dyes with Optimized Electron-Withdrawing Acceptors

10.1039/d3tb02124f · ExternalCitation · doi-reference

A Dual-Response Fluorescent Probe Rh-O-QL for Simultaneous Monitoring of NAD(P)H and pH during Mitochondrial Autophagy

10.1039/d5cc00961h · ExternalCitation · doi-reference

Harnessing the Power of Iridium AIEgens for NAD(P)H Detection in Aqueous Medium and Living Cells

10.1039/d5cc01124h · ExternalCitation · doi-reference

A NAD(P)H-Driven Mitochondria-to-Nucleus Probe for Dual-Organelle Imaging in Dopaminergic Toxicity Models

10.1039/d5cc03654b · ExternalCitation · doi-reference

Methylquinolinium-Enhanced near-Infrared Hemicyanine Dye for Ratiometric NAD(P)H Sensing in Live Cells via Carbon–Carbon Bond Conjugation

10.1039/d5tb00610d · ExternalCitation · doi-reference

Imidazolium-Tethered Red-Emissive Iridium AIEgen for Sensitive Detection of NAD(P)H in Aqueous and Cellular Media

10.1039/d5tb02044a · ExternalCitation · doi-reference

The Power to Reduce: Pyridine Nucleotides–Small Molecules with a Multitude of Functions

10.1042/bj20061638 · ExternalCitation · doi-reference

How Mitochondria Produce Reactive Oxygen Species

10.1042/bj20081386 · ExternalCitation · doi-reference

In Vivo Multiphoton Microscopy of NADH and FAD Redox States, Fluorescence Lifetimes, and Cellular Morphology in Precancerous Epithelia

10.1073/pnas.0708425104 · ExternalCitation · doi-reference

Phasor Approach to Fluorescence Lifetime Microscopy Distinguishes Different Metabolic States of Germ Cells in a Live Tissue

10.1073/pnas.1108161108 · ExternalCitation · doi-reference

Seeing Is Believing? A Beginners’ Guide to Practical Pitfalls in Image Acquisition

10.1083/jcb.200507103 · ExternalCitation · doi-reference

Designing a Rigorous Microscopy Experiment: Validating Methods and Avoiding Bias

10.1083/jcb.201812109 · ExternalCitation · doi-reference

NAD+/NADH and NADP+/NADPH in Cellular Functions and Cell Death: Regulation and Biological Consequences

10.1089/ars.2007.1672 · ExternalCitation · doi-reference

The Redox Code

10.1089/ars.2015.6247 · ExternalCitation · doi-reference

Challenges and Opportunities for Small-Molecule Fluorescent Probes in Redox Biology Applications

10.1089/ars.2017.7491 · ExternalCitation · doi-reference

The Return of Metabolism: Biochemistry and Physiology of the Pentose Phosphate Pathway

10.1111/brv.12140 · ExternalCitation · doi-reference

Fluorescence Lifetime Imaging Microscopy: Fundamentals and Advances in Instrumentation, Analysis, and Applications

10.1117/1.jbo.25.7.071203 · ExternalCitation · doi-reference

Consensus Guidelines for Cellular Label-Free Optical Metabolic Imaging: Ensuring Accuracy and Reproducibility in Metabolic Profiling

10.1117/1.jbo.30.s2.s23901 · ExternalCitation · doi-reference

Fundamentals of Cancer Metabolism

10.1126/sciadv.1600200 · ExternalCitation · doi-reference

Apollo-NADP(+) Reveals In Vivo Adaptation of NADPH/NADP(+) Metabolism in Electrically Activated Pancreatic β Cells

10.1126/sciadv.adi8317 · ExternalCitation · doi-reference

Identification of a Novel NADPH Generation Reaction in the Pentose Phosphate Pathway in Escherichia coli Using mBFP

10.1128/jb.00276-24 · ExternalCitation · doi-reference

Label-Free Optical Metabolic Imaging in Cells and Tissues

10.1146/annurev-bioeng-071516-044730 · ExternalCitation · doi-reference

Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism

10.1146/annurev-biophys-030722-021359 · ExternalCitation · doi-reference

Aerobic Glycolysis: Meeting the Metabolic Requirements of Cell Proliferation

10.1146/annurev-cellbio-092910-154237 · ExternalCitation · doi-reference

A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging

10.1146/annurev-physiol-022516-034055 · ExternalCitation · doi-reference

Targeting Antioxidants to Mitochondria by Conjugation to Lipophilic Cations

10.1146/annurev.pharmtox.47.120505.105110 · ExternalCitation · doi-reference

A Practical Guide to Evaluating Colocalization in Biological Microscopy

10.1152/ajpcell.00462.2010 · ExternalCitation · doi-reference

Quantitative Optical Imaging of Primary Tumor Organoid Metabolism Predicts Drug Response in Breast Cancer

10.1158/0008-5472.can-14-0663 · ExternalCitation · doi-reference

CellProfiler: Image Analysis Software for Identifying and Quantifying Cell Phenotypes

10.1186/gb-2006-7-10-r100 · ExternalCitation · doi-reference

10.1186/s12859-017-1934-z

10.1186/s12859-017-1934-z · ExternalCitation · doi-reference

Mitochondria Dysfunction in the Pathogenesis of Alzheimer’s Disease: Recent Advances

10.1186/s13024-020-00376-6 · ExternalCitation · doi-reference

Optical Metabolic Imaging Quantifies Heterogeneous Cell Populations

10.1364/boe.6.000559 · ExternalCitation · doi-reference

10.1371/journal.pone.0212061

10.1371/journal.pone.0212061 · ExternalCitation · doi-reference

Mitochondrial Membrane Potential Probes and the Proton Gradient: A Practical Usage Guide

10.2144/000113610 · ExternalCitation · doi-reference

10.3389/fmicb.2018.02564

10.3389/fmicb.2018.02564 · ExternalCitation · doi-reference

10.3390/ijms20174200

10.3390/ijms20174200 · ExternalCitation · doi-reference