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References from Engineering Programmable Expression Systems in Aspergillus: Genetic Architecture, Expression Control, Protein Secretion, and Chassis Optimization. Local targets link to admitted publications; unresolved targets remain external evidence.
Growing a circular economy with fungal biotechnology: A white paper
10.1186/s40694-020-00095-z · 2020 · External reference
Heterologous protein production in filamentous fungi
10.1007/s00253-023-12660-8 · 2023 · External reference
Recent advances in the development of Aspergillus for protein production
10.1016/j.biortech.2022.126768 · 2022 · External reference
10.3390/jof11070534
10.3390/jof11070534 · External reference
How a fungus shapes biotechnology: 100 years of Aspergillus niger research
10.1186/s40694-018-0054-5 · 2018 · External reference
Developing Aspergillus niger as a cell factory for food enzyme production
10.1016/j.biotechadv.2020.107630 · 2020 · External reference
Development of an efficient heterologous protein expression platform in Aspergillus niger through genetic modification of a glucoamylase hyperproducing industrial strain
10.1186/s12934-025-02786-x · 2025 · External reference
Multidimensional strategies for efficient heterologous protein expression in Aspergillus niger
10.1016/j.biotechadv.2025.108690 · 2025 · External reference
Genome sequencing and analysis of Aspergillus oryzae
10.1038/nature04300 · 2005 · External reference
Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit
10.1038/s41467-024-46314-8 · 2024 · External reference
Deciphering chemical logic of fungal natural product biosynthesis through heterologous expression and genome mining
10.1039/d2np00050d · 2023 · External reference
Versatile filamentous fungal host highly-producing heterologous natural products developed by genome editing-mediated engineering of multiple metabolic pathways
10.1038/s42003-024-06958-0 · 2024 · External reference
10.3390/jof10010034
10.3390/jof10010034 · External reference
Aspergillus nidulans
10.1016/j.tim.2022.09.013 · 2023 · External reference
Modular synthetic biology toolkit for filamentous fungi
10.1021/acssynbio.1c00260 · 2021 · External reference
Protein secretion and associated stress in industrially employed filamentous fungi
10.1007/s00253-023-12985-4 · 2024 · External reference
Marker and promoter effects on heterologous expression in Aspergillus nidulans
10.1007/s00253-006-0368-8 · 2006 · External reference
Chromosomal location plays a role in regulation of aflatoxin gene expression in Aspergillus parasiticus
10.1128/aem.68.1.306-315.2002 · 2002 · External reference
A CRISPR/Cas9-based multicopy integration system for protein production in Aspergillus niger
10.1111/febs.16891 · 2023 · External reference
Improvement of foreign-protein production in Aspergillus niger var. awamori by constitutive induction of the unfolded-protein response
10.1128/aem.69.12.6979-6986.2003 · 2003 · External reference
Effects of a defective ERAD pathway on growth and heterologous protein production in Aspergillus niger
10.1007/s00253-010-2916-5 · 2011 · External reference
Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability
10.1016/j.mtbio.2023.100560 · 2023 · External reference
Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus
10.1186/s13059-017-1151-0 · 2017 · External reference
Engineering strategies for enhanced heterologous protein production by Saccharomyces cerevisiae
10.1186/s12934-024-02299-z · 2024 · External reference
Advancing recombinant protein expression in Komagataella phaffii: Opportunities and challenges
10.1093/femsyr/foaf010 · 2025 · External reference
10.3390/microorganisms12020346
10.3390/microorganisms12020346 · External reference
A simple and universal system for gene manipulation in Aspergillus fumigatus: In vitro-assembled Cas9-guide RNA ribonucleoproteins coupled with microhomology repair templates
10.1128/msphere.00446-17 · 2017 · External reference
10.1371/journal.pone.0133085
10.1371/journal.pone.0133085 · External reference
Agrobacterium tumefaciens-mediated transformation of filamentous fungi
10.1038/nbt0998-839 · 1998 · External reference
A dual selection marker transformation system using Agrobacterium tumefaciens for the industrial Aspergillus oryzae 3.042
10.4014/jmb.1811.11027 · 2019 · External reference
Influence of α-1, 3-glucan synthase gene agsE on protoplast formation for transformation of Aspergillus luchuensis
10.1016/j.jbiosc.2019.01.018 · 2019 · External reference
Next-generation AMA1-based plasmids for enhanced heterologous expression in filamentous fungi
10.1111/1751-7915.70010 · 2024 · External reference
Forced recycling of an AMA1-based genome-editing plasmid allows for efficient multiple gene deletion/integration in the industrial filamentous fungus Aspergillus oryzae
10.1128/aem.01896-18 · 2019 · External reference
Moulding the mould: Understanding and reprogramming filamentous fungal growth and morphogenesis for next generation cell factories
10.1186/s13068-019-1400-4 · 2019 · External reference
Understanding and controlling filamentous growth of fungal cell factories: Novel tools and opportunities for targeted morphology engineering
10.1186/s40694-021-00115-6 · 2021 · External reference
The filamentous fungal pellet—Relationship between morphology and productivity
10.1007/s00253-018-8818-7 · 2018 · External reference
Localization of growth and secretion of proteins in Aspergillus niger
1991 · External reference
Morphological regulation of Aspergillus niger to improve citric acid production by chsC gene silencing
10.1007/s00449-018-1932-1 · 2018 · External reference
Cultivation of filamentous fungi in airlift bioreactors: Advantages and disadvantages
10.1007/s00253-025-13422-4 · 2025 · External reference
Transformation by integration in Aspergillus nidulans
10.1016/0378-1119(83)90191-9 · 1983 · External reference
NHEJ and HDR can occur simultaneously during gene integration into the genome of Aspergillus niger
10.1186/s40694-024-00180-7 · 2024 · External reference
Ku80 gene is related to non-homologous end-joining and genome stability in Aspergillus niger
10.1007/s00284-010-9853-5 · 2011 · External reference
10.1186/s12915-019-0702-0
10.1186/s12915-019-0702-0 · External reference
Dual-single-guide RNA strategy improves CRISPR-mediated homology-directed repair in Aspergillus
10.1093/nar/gkag095 · 2026 · External reference
CRISPR-Cas9 cytidine-base-editor mediated continuous in vivo evolution in Aspergillus nidulans
10.1021/acssynbio.4c00716 · 2025 · External reference
10.1038/s41587-026-03202-4
10.1038/s41587-026-03202-4 · External reference
Versatile enzyme expression and characterization system for Aspergillus nidulans, with the Penicillium brevicompactum polyketide synthase gene from the mycophenolic acid gene cluster as a test case
10.1128/aem.01768-10 · 2011 · External reference
10.1371/journal.pone.0072871
10.1371/journal.pone.0072871 · External reference
Transcriptional and post-transcriptional events affect the production of secreted hen egg white lysozyme by Aspergillus niger
10.1007/bf01973589 · 1994 · External reference
RoCi—A single-step multi-copy integration system based on rolling-circle replication
10.1093/nar/gkaf1488 · 2026 · External reference
Telomere position effect is regulated by heterochromatin-associated proteins and NkuA in Aspergillus nidulans
10.1099/mic.0.039255-0 · 2010 · External reference
Translocated duplication of a targeted chromosomal segment enhances gene expression at the duplicated site and results in phenotypic changes in Aspergillus oryzae
10.1186/s40694-018-0061-6 · 2018 · External reference
Tet-on, or Tet-off, that is the question: Advanced conditional gene expression in Aspergillus
10.1016/j.fgb.2015.11.003 · 2016 · External reference
Fungal gene expression on demand: An inducible, tunable, and metabolism-independent expression system for Aspergillus niger
10.1128/aem.02740-10 · 2011 · External reference
Promoter engineering with programmable upstream activating sequences in Aspergillus niger cell factory
10.1186/s12934-025-02642-y · 2025 · External reference
CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi
10.1021/acssynbio.0c00197 · 2020 · External reference
10.3390/jof8070679
10.3390/jof8070679 · External reference
Translation efficiency mediated by the 5′ untranslated region greatly affects protein production in Aspergillus oryzae
10.1007/s00253-004-1681-8 · 2004 · External reference
Enhancing the yield of human erythropoietin in Aspergillus niger by introns and CRISPR-Cas9
10.1016/j.pep.2020.105570 · 2020 · External reference
Codon optimization increases steady-state mRNA levels in Aspergillus oryzae heterologous gene expression
10.1128/aem.01354-08 · 2008 · External reference
Profiling heterologous 3′ terminator-dependent expression variation in Aspergillus oryzae
10.1016/j.synbio.2026.04.033 · 2026 · External reference
Synthetic biology tools for bioprospecting of natural products in eukaryotes
10.1016/j.chembiol.2014.02.010 · 2014 · External reference
Polycistronic gene expression in Aspergillus niger
10.1186/s12934-017-0780-z · 2017 · External reference
Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post) translational levels
10.1128/aem.63.2.488-497.1997 · 1997 · External reference
Determinants of the fidelity of processing glucoamylase-lysozyme fusions by Aspergillus niger
10.1046/j.1432-1327.1998.2580107.x · 1998 · External reference
Protein disulfide isomerase 1 is required for RodA assembling-based conidial hydrophobicity of Aspergillus fumigatus
10.1128/aem.01260-23 · 2024 · External reference
Glycoengineering of Aspergillus nidulans to produce precursors for humanized N-glycan structures
10.1016/j.ymben.2021.06.001 · 2021 · External reference
The mechanistic impact of N-glycosylation on stability, pharmacokinetics, and immunogenicity of therapeutic proteins
10.1016/j.xphs.2018.11.029 · 2019 · External reference
Disruption of ten protease genes in the filamentous fungus Aspergillus oryzae highly improves production of heterologous proteins
10.1007/s00253-010-2937-0 · 2011 · External reference
Overexpression of msnA reprograms metabolism to enhance recombinant protein secretion across Aspergillus species
10.1186/s12934-026-02975-2 · 2026 · External reference
Mycotoxin-free Aspergillus oryzae strain lineage for alternative and novel protein production at industrial scale
10.1007/s00253-025-13482-6 · 2025 · External reference
Functional production of human antibody by the filamentous fungus Aspergillus oryzae
10.1186/s40694-020-00098-w · 2020 · External reference
High-throughput droplet microfluidics screening and genome sequencing analysis for improved amylase-producing Aspergillus oryzae
10.1186/s13068-023-02437-6 · 2023 · External reference
Microbioreactor-assisted cultivation workflows for time-efficient phenotyping of protein producing Aspergillus niger in batch and fed-batch mode
2021 · External reference
High-throughput culture and DNA isolation methods for Aspergillus fumigatus
10.1002/cpz1.70112 · 2025 · External reference
Precise prediction of promoter strength based on a de novo synthetic promoter library coupled with machine learning
10.1021/acssynbio.1c00117 · 2022 · External reference
Technologies for design-build-test-learn automation and computational modelling across the synthetic biology workflow: A review
10.1007/s13721-024-00455-4 · 2024 · External reference
High-throughput culture and DNA isolation methods for Aspergillus fumigatus
10.1002/cpz1.70112 · ExternalCitation · doi-reference
Transcriptional and post-transcriptional events affect the production of secreted hen egg white lysozyme by Aspergillus niger
10.1007/bf01973589 · ExternalCitation · doi-reference
Translation efficiency mediated by the 5′ untranslated region greatly affects protein production in Aspergillus oryzae
10.1007/s00253-004-1681-8 · ExternalCitation · doi-reference
Marker and promoter effects on heterologous expression in Aspergillus nidulans
10.1007/s00253-006-0368-8 · ExternalCitation · doi-reference
Effects of a defective ERAD pathway on growth and heterologous protein production in Aspergillus niger
10.1007/s00253-010-2916-5 · ExternalCitation · doi-reference
Disruption of ten protease genes in the filamentous fungus Aspergillus oryzae highly improves production of heterologous proteins
10.1007/s00253-010-2937-0 · ExternalCitation · doi-reference
The filamentous fungal pellet—Relationship between morphology and productivity
10.1007/s00253-018-8818-7 · ExternalCitation · doi-reference
Heterologous protein production in filamentous fungi
10.1007/s00253-023-12660-8 · ExternalCitation · doi-reference
Protein secretion and associated stress in industrially employed filamentous fungi
10.1007/s00253-023-12985-4 · ExternalCitation · doi-reference
Cultivation of filamentous fungi in airlift bioreactors: Advantages and disadvantages
10.1007/s00253-025-13422-4 · ExternalCitation · doi-reference
Mycotoxin-free Aspergillus oryzae strain lineage for alternative and novel protein production at industrial scale
10.1007/s00253-025-13482-6 · ExternalCitation · doi-reference
Ku80 gene is related to non-homologous end-joining and genome stability in Aspergillus niger
10.1007/s00284-010-9853-5 · ExternalCitation · doi-reference
Morphological regulation of Aspergillus niger to improve citric acid production by chsC gene silencing
10.1007/s00449-018-1932-1 · ExternalCitation · doi-reference
Technologies for design-build-test-learn automation and computational modelling across the synthetic biology workflow: A review
10.1007/s13721-024-00455-4 · ExternalCitation · doi-reference
Transformation by integration in Aspergillus nidulans
10.1016/0378-1119(83)90191-9 · ExternalCitation · doi-reference
Recent advances in the development of Aspergillus for protein production
10.1016/j.biortech.2022.126768 · ExternalCitation · doi-reference
Developing Aspergillus niger as a cell factory for food enzyme production
10.1016/j.biotechadv.2020.107630 · ExternalCitation · doi-reference
Multidimensional strategies for efficient heterologous protein expression in Aspergillus niger
10.1016/j.biotechadv.2025.108690 · ExternalCitation · doi-reference
Synthetic biology tools for bioprospecting of natural products in eukaryotes
10.1016/j.chembiol.2014.02.010 · ExternalCitation · doi-reference
Tet-on, or Tet-off, that is the question: Advanced conditional gene expression in Aspergillus
10.1016/j.fgb.2015.11.003 · ExternalCitation · doi-reference
Influence of α-1, 3-glucan synthase gene agsE on protoplast formation for transformation of Aspergillus luchuensis
10.1016/j.jbiosc.2019.01.018 · ExternalCitation · doi-reference
Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability
10.1016/j.mtbio.2023.100560 · ExternalCitation · doi-reference
Enhancing the yield of human erythropoietin in Aspergillus niger by introns and CRISPR-Cas9
10.1016/j.pep.2020.105570 · ExternalCitation · doi-reference
Profiling heterologous 3′ terminator-dependent expression variation in Aspergillus oryzae
10.1016/j.synbio.2026.04.033 · ExternalCitation · doi-reference
Aspergillus nidulans
10.1016/j.tim.2022.09.013 · ExternalCitation · doi-reference
The mechanistic impact of N-glycosylation on stability, pharmacokinetics, and immunogenicity of therapeutic proteins
10.1016/j.xphs.2018.11.029 · ExternalCitation · doi-reference
Glycoengineering of Aspergillus nidulans to produce precursors for humanized N-glycan structures
10.1016/j.ymben.2021.06.001 · ExternalCitation · doi-reference
CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi
10.1021/acssynbio.0c00197 · ExternalCitation · doi-reference
Precise prediction of promoter strength based on a de novo synthetic promoter library coupled with machine learning
10.1021/acssynbio.1c00117 · ExternalCitation · doi-reference
Modular synthetic biology toolkit for filamentous fungi
10.1021/acssynbio.1c00260 · ExternalCitation · doi-reference
CRISPR-Cas9 cytidine-base-editor mediated continuous in vivo evolution in Aspergillus nidulans
10.1021/acssynbio.4c00716 · ExternalCitation · doi-reference
Genome sequencing and analysis of Aspergillus oryzae
10.1038/nature04300 · ExternalCitation · doi-reference
Agrobacterium tumefaciens-mediated transformation of filamentous fungi
10.1038/nbt0998-839 · ExternalCitation · doi-reference
Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit
10.1038/s41467-024-46314-8 · ExternalCitation · doi-reference
10.1038/s41587-026-03202-4
10.1038/s41587-026-03202-4 · ExternalCitation · doi-reference
Versatile filamentous fungal host highly-producing heterologous natural products developed by genome editing-mediated engineering of multiple metabolic pathways
10.1038/s42003-024-06958-0 · ExternalCitation · doi-reference
Deciphering chemical logic of fungal natural product biosynthesis through heterologous expression and genome mining
10.1039/d2np00050d · ExternalCitation · doi-reference
Determinants of the fidelity of processing glucoamylase-lysozyme fusions by Aspergillus niger
10.1046/j.1432-1327.1998.2580107.x · ExternalCitation · doi-reference
Advancing recombinant protein expression in Komagataella phaffii: Opportunities and challenges
10.1093/femsyr/foaf010 · ExternalCitation · doi-reference
RoCi—A single-step multi-copy integration system based on rolling-circle replication
10.1093/nar/gkaf1488 · ExternalCitation · doi-reference
Dual-single-guide RNA strategy improves CRISPR-mediated homology-directed repair in Aspergillus
10.1093/nar/gkag095 · ExternalCitation · doi-reference
Telomere position effect is regulated by heterochromatin-associated proteins and NkuA in Aspergillus nidulans
10.1099/mic.0.039255-0 · ExternalCitation · doi-reference
Next-generation AMA1-based plasmids for enhanced heterologous expression in filamentous fungi
10.1111/1751-7915.70010 · ExternalCitation · doi-reference
A CRISPR/Cas9-based multicopy integration system for protein production in Aspergillus niger
10.1111/febs.16891 · ExternalCitation · doi-reference
Protein disulfide isomerase 1 is required for RodA assembling-based conidial hydrophobicity of Aspergillus fumigatus
10.1128/aem.01260-23 · ExternalCitation · doi-reference
Codon optimization increases steady-state mRNA levels in Aspergillus oryzae heterologous gene expression
10.1128/aem.01354-08 · ExternalCitation · doi-reference
Versatile enzyme expression and characterization system for Aspergillus nidulans, with the Penicillium brevicompactum polyketide synthase gene from the mycophenolic acid gene cluster as a test case
10.1128/aem.01768-10 · ExternalCitation · doi-reference
Forced recycling of an AMA1-based genome-editing plasmid allows for efficient multiple gene deletion/integration in the industrial filamentous fungus Aspergillus oryzae
10.1128/aem.01896-18 · ExternalCitation · doi-reference
Fungal gene expression on demand: An inducible, tunable, and metabolism-independent expression system for Aspergillus niger
10.1128/aem.02740-10 · ExternalCitation · doi-reference
Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post) translational levels
10.1128/aem.63.2.488-497.1997 · ExternalCitation · doi-reference
Chromosomal location plays a role in regulation of aflatoxin gene expression in Aspergillus parasiticus
10.1128/aem.68.1.306-315.2002 · ExternalCitation · doi-reference
Improvement of foreign-protein production in Aspergillus niger var. awamori by constitutive induction of the unfolded-protein response
10.1128/aem.69.12.6979-6986.2003 · ExternalCitation · doi-reference
A simple and universal system for gene manipulation in Aspergillus fumigatus: In vitro-assembled Cas9-guide RNA ribonucleoproteins coupled with microhomology repair templates
10.1128/msphere.00446-17 · ExternalCitation · doi-reference
10.1186/s12915-019-0702-0
10.1186/s12915-019-0702-0 · ExternalCitation · doi-reference
Polycistronic gene expression in Aspergillus niger
10.1186/s12934-017-0780-z · ExternalCitation · doi-reference
Engineering strategies for enhanced heterologous protein production by Saccharomyces cerevisiae
10.1186/s12934-024-02299-z · ExternalCitation · doi-reference
Promoter engineering with programmable upstream activating sequences in Aspergillus niger cell factory
10.1186/s12934-025-02642-y · ExternalCitation · doi-reference
Development of an efficient heterologous protein expression platform in Aspergillus niger through genetic modification of a glucoamylase hyperproducing industrial strain
10.1186/s12934-025-02786-x · ExternalCitation · doi-reference
Overexpression of msnA reprograms metabolism to enhance recombinant protein secretion across Aspergillus species
10.1186/s12934-026-02975-2 · ExternalCitation · doi-reference
Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus
10.1186/s13059-017-1151-0 · ExternalCitation · doi-reference
Moulding the mould: Understanding and reprogramming filamentous fungal growth and morphogenesis for next generation cell factories
10.1186/s13068-019-1400-4 · ExternalCitation · doi-reference
High-throughput droplet microfluidics screening and genome sequencing analysis for improved amylase-producing Aspergillus oryzae
10.1186/s13068-023-02437-6 · ExternalCitation · doi-reference
How a fungus shapes biotechnology: 100 years of Aspergillus niger research
10.1186/s40694-018-0054-5 · ExternalCitation · doi-reference
Translocated duplication of a targeted chromosomal segment enhances gene expression at the duplicated site and results in phenotypic changes in Aspergillus oryzae
10.1186/s40694-018-0061-6 · ExternalCitation · doi-reference
Growing a circular economy with fungal biotechnology: A white paper
10.1186/s40694-020-00095-z · ExternalCitation · doi-reference
Functional production of human antibody by the filamentous fungus Aspergillus oryzae
10.1186/s40694-020-00098-w · ExternalCitation · doi-reference
Understanding and controlling filamentous growth of fungal cell factories: Novel tools and opportunities for targeted morphology engineering
10.1186/s40694-021-00115-6 · ExternalCitation · doi-reference
NHEJ and HDR can occur simultaneously during gene integration into the genome of Aspergillus niger
10.1186/s40694-024-00180-7 · ExternalCitation · doi-reference
10.1371/journal.pone.0072871
10.1371/journal.pone.0072871 · ExternalCitation · doi-reference
10.1371/journal.pone.0133085
10.1371/journal.pone.0133085 · ExternalCitation · doi-reference
10.3390/jof10010034
10.3390/jof10010034 · ExternalCitation · doi-reference
10.3390/jof11070534
10.3390/jof11070534 · ExternalCitation · doi-reference
10.3390/jof8070679
10.3390/jof8070679 · ExternalCitation · doi-reference
10.3390/microorganisms12020346
10.3390/microorganisms12020346 · ExternalCitation · doi-reference
A dual selection marker transformation system using Agrobacterium tumefaciens for the industrial Aspergillus oryzae 3.042
10.4014/jmb.1811.11027 · ExternalCitation · doi-reference
