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References from In extracto cryo-EM reveals eEF2 as a major hibernation factor on 60S and 80S particles. Local targets link to admitted publications; unresolved targets remain external evidence.
Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome
10.7554/elife.14874 · 2016 · External reference
Specific recognition and ubiquitination of translating ribosomes by mammalian CCR4–NOT
10.1038/s41594-023-01075-8 · 2023 · External reference
The Phenix software for automated determination of macromolecular structures
10.1016/j.ymeth.2011.07.005 · 2011 · External reference
Real-space refinement in PHENIX for cryo-EM and crystallography
10.1107/s2059798318006551 · 2018 · External reference
ZNF574 is a quality control factor for defective ribosome biogenesis intermediates
10.1016/j.molcel.2025.04.017 · 2025 · External reference
Glucose depletion rapidly inhibits translation initiation in yeast
10.1091/mbc.11.3.833 · 2000 · External reference
Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles
10.7554/elife.00461 · 2013 · External reference
Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography
10.1038/s41592-025-02659-6 · 2025 · External reference
Structural snapshots of actively translating human ribosomes
10.1016/j.cell.2015.03.052 · 2015 · External reference
Dynamic association of human Ebp1 with the ribosome
10.1261/rna.077602.120 · 2021 · External reference
TRIM37 is a new histone H2A ubiquitin ligase and breast cancer oncoprotein
10.1038/nature13955 · 2014 · External reference
Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling
10.1016/j.cell.2023.12.027 · 2024 · External reference
Structures of translationally inactive mammalian ribosomes
10.7554/elife.40486 · 2018 · External reference
Movies of ice-embedded particles enhance resolution in electron cryo-microscopy
10.1016/j.str.2012.08.026 · 2012 · External reference
Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP
10.1038/s41467-021-27415-0 · 2021 · External reference
Ribosome-associated vesicles: a dynamic subcompartment of the endoplasmic reticulum in secretory cells
10.1126/sciadv.aay9572 · 2020 · External reference
Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA
10.1038/s41467-023-36175-y · 2023 · External reference
Three tRNAs on the ribosome slow translation elongation
10.1073/pnas.1719592115 · 2017 · External reference
Better, faster, cheaper: recent advances in cryo-electron microscopy
10.1146/annurev-biochem-032620-110705 · 2022 · External reference
Spontaneous intersubunit rotation in single ribosomes
10.1016/j.molcel.2008.05.004 · 2008 · External reference
Mammalian Translation Termination Intermediates Captured Using PDMS Microfluidics-Based Time-Resolved Cryo-EM (TRCEM)
2024 · External reference
The highly conserved eukaryotic DRG factors are required for efficient translation in a manner redundant with the putative RNA helicase Slh1
10.1093/nar/gkq898 · 2011 · External reference
Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation
10.1038/s41467-021-24911-1 · 2021 · External reference
Convolutional networks for supervised mining of molecular patterns within cellular context
10.1038/s41592-022-01746-2 · 2023 · External reference
Phosphorylation of initiation factor 2 alpha by protein kinase GCN2 mediates gene-specific translational control of GCN4 in yeast
10.1016/0092-8674(92)90193-g · 1992 · External reference
Accuracy mechanism of eukaryotic ribosome translocation
10.1038/s41586-021-04131-9 · 2021 · External reference
The eIF2α kinases: their structures and functions
10.1007/s00018-012-1252-6 · 2013 · External reference
Implication of Stm1 in the protection of eIF5A, eEF2 and tRNA through dormant ribosomes
10.3389/fmolb.2024.1395220 · 2024 · External reference
Hibernating ribosomes as drug targets?
10.3389/fmicb.2024.1436579 · 2024 · External reference
CTFFIND5 provides improved insight into quality, tilt, and thickness of TEM samples
10.7554/elife.97227 · 2024 · External reference
Yafw
2026 · External reference
mRNA translocation occurs during the second step of ribosomal intersubunit rotation
10.1038/nsmb.2011 · 2011 · External reference
Digitonin does not flip across cholesterol-poor membranes
10.1016/j.jcis.2017.05.034 · 2017 · External reference
Visualization of translation reorganization upon persistent ribosome collision stress in mammalian cells
10.1016/j.molcel.2024.01.015 · 2024 · External reference
tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis
10.1016/j.celrep.2018.11.040 · 2018 · External reference
Transient disome complex formation in native polysomes during ongoing protein synthesis captured by cryo-EM
10.1038/s41467-024-46092-3 · 2024 · External reference
La-related Protein 1 (LARP1) represses Terminal Oligopyrimidine (TOP) mRNA translation downstream of mTOR Complex 1 (mTORC1)
10.1074/jbc.m114.621730 · 2015 · External reference
Key intermediates in ribosome recycling visualized by time-resolved cryoelectron microscopy
10.1016/j.str.2016.09.014 · 2016 · External reference
The structural basis for release factor activation during translation termination revealed by time-resolved cryogenic electron microscopy
10.1016/j.bpj.2018.11.3090 · 2019 · External reference
The structure of the ribosome with elongation factor G trapped in the posttranslocational state
10.1126/science.1179709 · 2009 · External reference
Visualization of translation and protein biogenesis at the ER membrane
10.1038/s41586-022-05638-5 · 2023 · External reference
Survival of the drowsiest: the hibernating 100S ribosome in bacterial stress management
10.1007/s00294-017-0796-2 · 2018 · External reference
cisTEM, user-friendly software for single-particle image processing
10.7554/elife.35383 · 2018 · External reference
Insertion of the biogenesis factor Rei1 probes the ribosomal tunnel during 60S maturation
10.1016/j.cell.2015.11.027 · 2016 · External reference
Regulated translation initiation controls stress-induced gene expression in mammalian cells
10.1016/s1097-2765(00)00108-8 · 2000 · External reference
High-throughput cryo-electron tomography enables multiscale visualization of the inner life of microbes
10.1016/j.sbi.2025.103065 · 2025 · External reference
Cryo-TEM simulations of amorphous radiation-sensitive samples using multislice wave propagation
10.1107/s2052252521008538 · 2021 · External reference
Factor dependent breakdown of polysomes
10.1016/0006-291x(70)90165-8 · 1970 · External reference
Structures of the eukaryotic ribosome and its translational states in situ
10.1038/s41467-022-34997-w · 2022 · External reference
LARP1 functions as a molecular switch for mTORC1-mediated translation of an essential class of mRNAs
10.7554/elife.25237 · 2017 · External reference
eIF6 rebinding dynamically couples ribosome maturation and translation
10.1038/s41467-022-29214-7 · 2022 · External reference
Molecular basis of the TRAP complex function in ER protein biogenesis
10.1038/s41594-023-00990-0 · 2023 · External reference
Structural aspects of messenger RNA reading frame maintenance by the ribosome
10.1038/nsmb.1790 · 2010 · External reference
Highly accurate protein structure prediction with AlphaFold
10.1038/s41586-021-03819-2 · 2021 · External reference
Late steps in bacterial translation initiation visualized using time-resolved cryo-EM
10.1038/s41586-019-1249-5 · 2019 · External reference
Eukaryotic elongation factor-2 (eEF2): its regulation and peptide chain elongation
10.1002/cbf.1740 · 2011 · External reference
Swinging lever mechanism of myosin directly shown by time-resolved cryo-EM
10.1038/s41586-025-08876-5 · 2025 · External reference
Biogenesis of erythrocyte membrane proteins in vitro studies with rabbit reticulocytes
10.1016/0005-2736(75)90395-8 · 1975 · External reference
Ribosomal dormancy at the nexus of ribosome homeostasis and protein synthesis
10.1002/bies.202300247 · 2024 · External reference
Structural dynamics of the ribosome
10.1016/j.cbpa.2008.08.037 · 2008 · External reference
The structural dynamics of translation
10.1146/annurev-biochem-071921-122857 · 2022 · External reference
Autonomous ribosome biogenesis in vitro
10.1038/s41467-025-55853-7 · 2025 · External reference
Computer visualization of three-dimensional image data using IMOD
10.1006/jsbi.1996.0013 · 1996 · External reference
Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM
10.1038/nmeth.2472 · 2013 · External reference
Structure of the TRPV1 ion channel determined by electron cryo-microscopy
10.1038/nature12822 · 2013 · External reference
Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix
10.1107/s2059798319011471 · 2019 · External reference
Structural insights into ribosome translocation
10.1002/wrna.1354 · 2016 · External reference
Hibernation-promoting factor sequesters Staphylococcus aureus ribosomes to antagonize RNase R-mediated nucleolytic degradation
10.1128/mbio.00334-21 · 2021 · External reference
Biosynthesis of reticulocyte membrane proteins by membrane-free polyribosomes
10.1073/pnas.70.5.1526 · 1973 · External reference
Cryo-EM of elongating ribosome with EF-Tu•GTP elucidates tRNA proofreading
10.1038/s41586-020-2447-x · 2020 · External reference
Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM
10.1038/s41467-022-30418-0 · 2022 · External reference
Structural mechanism of angiogenin activation by the ribosome
10.1038/s41586-024-07508-8 · 2024 · External reference
Locating macromolecular assemblies in cells by 2D template matching with cisTEM
10.7554/elife.68946 · 2021 · External reference
Likelihood-based classification of cryo-EM images using FREALIGN
10.1016/j.jsb.2013.07.005 · 2013 · External reference
Sudden depletion of carbon source blocks translation, but not transcription, in the yeast Saccharomyces cerevisiae
10.1016/0014-5793(96)00683-7 · 1996 · External reference
Automated electron microscope tomography using robust prediction of specimen movements
10.1016/j.jsb.2005.07.007 · 2005 · External reference
Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural states
10.1073/pnas.2521210123 · 2026 · External reference
UCSF ChimeraX: Tools for structure building and analysis
10.1002/pro.4792 · 2023 · External reference
Reversible membrane permeabilization of mammalian cells treated with digitonin and its use for inducing nuclear reprogramming by Xenopus egg extracts
10.1089/clo.2008.0020 · 2008 · External reference
Bridging structural and cell biology with cryo-electron microscopy
10.1038/s41586-024-07198-2 · 2024 · External reference
The ribosome comes to life
10.1016/j.cell.2024.10.035 · 2024 · External reference
Cryo-EM structure of the inactive ribosome complex accumulated in chick embryo cells in cold-stress conditions
10.1002/1873-3468.14831 · 2024 · External reference
An efficient mRNA-dependent translation system from reticulocyte lysates
10.1111/j.1432-1033.1976.tb10656.x · 1976 · External reference
The roles of individual eukaryotic translation initiation factors in ribosomal scanning and initiation codon selection
10.1101/gad.1020902 · 2002 · External reference
Structure of Gcn1 bound to stalled and colliding 80S ribosomes
10.1073/pnas.2022756118 · 2021 · External reference
Current data processing strategies for cryo-electron tomography and subtomogram averaging
10.1042/bcj20200715 · 2021 · External reference
Cryo-EM structure of Hepatitis C virus IRES bound to the human ribosome at 3.9-Å resolution
10.1038/ncomms8646 · 2015 · External reference
Visualization of two transfer RNAs trapped in transit during elongation factor G-mediated translocation
10.1073/pnas.1320387110 · 2013 · External reference
Back to basics: the untreated rabbit reticulocyte lysate as a competitive system to recapitulate cap/poly(A) synergy and the selective advantage of IRES-driven translation
10.1093/nar/gkm682 · 2007 · External reference
10.1007/978-3-7091-0215-2
10.1007/978-3-7091-0215-2 · 2011 · External reference
LARP1 binds ribosomes and TOP mRNAs in repressed complexes
10.1038/s44318-024-00294-z · 2024 · External reference
Movement of elongation factor G between compact and extended conformations
10.1016/j.jmb.2014.11.010 · 2015 · External reference
RELION: implementation of a Bayesian approach to cryo-EM structure determination
10.1016/j.jsb.2012.09.006 · 2012 · External reference
eIF5A functions globally in translation elongation and termination
10.1016/j.molcel.2017.03.003 · 2017 · External reference
Structural and mutational analysis of the ribosome-arresting human XBP1u
10.7554/elife.46267 · 2019 · External reference
Decoding mammalian ribosome-mRNA states by translational GTPase complexes
10.1016/j.cell.2016.10.046 · 2016 · External reference
Endonucleolytic cleavage in the expansion segment 7 of 25S rRNA is an early marker of low-level oxidative stress in yeast
10.1074/jbc.m117.800003 · 2017 · External reference
TORC1 phosphorylates and inhibits the ribosome preservation factor Stm1 to activate dormant ribosomes
10.15252/embj.2022112344 · 2023 · External reference
Functional role of the sarcin–ricin loop of the 23S rRNA in the elongation cycle of protein synthesis
10.1016/j.jmb.2012.03.016 · 2012 · External reference
Functionally distinct roles for eEF2K in the control of ribosome availability and p-body abundance
10.1038/s41467-021-27160-4 · 2021 · External reference
The space between notes: emerging roles for translationally silent ribosomes
10.1016/j.tibs.2022.02.003 · 2022 · External reference
The ribosome as a conveying thermal ratchet machine
10.1074/jbc.x109.001552 · 2009 · External reference
Ebp1 is a dsRNA-binding protein associated with ribosomes that modulates eIF2alpha phosphorylation
10.1016/j.bbrc.2006.03.205 · 2006 · External reference
In vitro and tissue culture methods for analysis of translation initiation on the endoplasmic reticulum
10.1016/s0076-6879(07)31004-5 · 2007 · External reference
Termi-Luc: a versatile assay to monitor full-protein release from ribosomes
10.1261/rna.076588.120 · 2020 · External reference
EMAN2: an extensible image processing suite for electron microscopy
10.1016/j.jsb.2006.05.009 · 2007 · External reference
Structures of modified eEF2 80S ribosome complexes reveal the role of GTP hydrolysis in translocation
10.1038/sj.emboj.7601677 · 2007 · External reference
Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2
10.1126/science.abc8665 · 2020 · External reference
Hibernation as a stage of ribosome functioning
10.1134/s0006297920110115 · 2020 · External reference
Structural basis of the translational elongation cycle
10.1146/annurev-biochem-113009-092313 · 2013 · External reference
Multiperspective smFRET reveals rate-determining late intermediates of ribosomal translocation
10.1038/nsmb.3177 · 2016 · External reference
Quantitation of intracellular membrane-bound enzymes and receptors in digitonin-permeabilized cells
10.1016/0003-2697(83)90106-9 · 1983 · External reference
Structure and function of yeast Lso2 and human CCDC124 bound to hibernating ribosomes
10.1371/journal.pbio.3000780 · 2020 · External reference
Oxidative stress damages rRNA inside the ribosome and differentially affects the catalytic center
10.1093/nar/gkx1308 · 2018 · External reference
SPIDR enables multiplexed mapping of RNA-protein interactions and uncovers a mechanism for selective translational suppression upon cell stress
10.1016/j.cell.2025.06.042 · 2025 · External reference
Translation dynamics in human cells visualized at high resolution reveal cancer drug action
10.1126/science.adh1411 · 2023 · External reference
Visualizing translation dynamics at atomic detail inside a bacterial cell
10.1038/s41586-022-05255-2 · 2022 · External reference
Adaptive response in stress granule formation and bulk translational repression upon a combined stress of mild heat shock and mild ethanol stress in yeast
10.1111/gtc.12090 · 2013 · External reference
Molecular architecture of the ribosome-bound Hepatitis C Virus internal ribosomal entry site RNA
10.15252/embj.201592469 · 2015 · External reference
Bringing structure to cell biology with cryo-electron tomography
10.1146/annurev-biophys-111622-091327 · 2023 · External reference
Conserved heterodimeric GTPase Rbg1/Tma46 promotes efficient translation in eukaryotic cells
10.1016/j.celrep.2021.109877 · 2021 · External reference
The trends of in situ focused ion beam technology: toward preparing transmission electron microscopy lamella and devices at the atomic scale
10.1002/aelm.202101401 · 2022 · External reference
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy
10.1038/nmeth.4193 · 2017 · External reference
Visualizing the translation landscape in human cells at high resolution
10.1038/s41467-025-65795-9 · 2025 · External reference
New tools for automated high-resolution cryo-EM structure determination in RELION-3
10.7554/elife.42166 · 2018 · External reference
Structural Basis for Non-AUG Translation Regulation by 5MPs
10.64898/2025.12.28.696766 · 2025 · External reference
Rabbit 80S ribosome with eEF2, eIF5a and SERBP1
10.2210/pdb11iq/pdb · 2026 · External reference
Rabbit 80S ribosome with eEF2, CCDC124 and LARP1
10.2210/pdb11hg/pdb · 2026 · External reference
Rabbit 80S ribosome with eEF2, IFRD2 and LARP1
10.2210/pdb11jj/pdb · 2026 · External reference
Rabbit 80S ribosome with IFRD2 and LARP1
10.2210/pdb11kh/pdb · 2026 · External reference
Rabbit 60S with eEF2, domain IV open
10.2210/pdb11hv/pdb · 2026 · External reference
Rabbit 60S with eEF2, domain IV closed
10.2210/pdb11he/pdb · 2026 · External reference
Rabbit 80S ribosome with eEF2, eIF5A and SERBP1
2026 · External reference
Rabbit 80S ribosome with eEF2, CCDC124 and LARP1
2026 · External reference
Rabbit 80S ribosome with eEF2, IFRD2 and LARP1
2026 · External reference
Rabbit 80S with IFRD2 and LARP1, 40S head-swiveled
2026 · External reference
Rabbit 60S with eEF2, domain IV open
2026 · External reference
Rabbit 60S with eEF2, domain IV closed
2026 · External reference
merged rabbit reticulocyte lysate data
2026 · External reference
In extracto cryo-EM reveals eEF2 as a major hibernation factor on 60S and 80S particles
2026 · External reference
MCF-7-starved cell lysate
2026 · External reference
In extracto cryo-EM reveals eEF2 as a major hibernation factor on 60S and 80S particles
2026 · External reference
Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20
10.2210/pdb7tor/pdb · 2022 · External reference
Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel)
10.2210/pdb6mtd/pdb · 2018 · External reference