Research graph
References from Gβγ engages PLCβ3 at multiple sites to reorient and facilitate its activation. Local targets link to admitted publications; unresolved targets remain external evidence.
PHENIX: a comprehensive Python-based system for macromolecular structure solution
10.1107/s0907444909052925 · 2010 · External reference
Oxidative stress contributes to the enhanced expression of Gqα/PLCβ1 proteins and hypertrophy of VSMC from SHR: role of growth factor receptor transactivation
10.1152/ajpheart.00659.2015 · 2016 · External reference
Proteolytic modification of membrane-associated phospholipase C-beta by mu-calpain enhances its activation by G-protein beta gamma subunits in human platelets
10.1016/0014-5793(94)80134-7 · 1994 · External reference
Identification of a region at the N-terminus of phospholipase C-beta 3 that interacts with G protein beta gamma subunits
10.1021/bi992021f · 2000 · External reference
Regulatory interactions between the amino terminus of G-protein betagamma subunits and the catalytic domain of phospholipase Cbeta2
10.1074/jbc.m412514200 · 2005 · External reference
A molecular mechanism to diversify Ca2+ signaling downstream of Gs protein-coupled receptors
10.1038/s41467-024-51991-6 · 2024 · External reference
Cell shape regulates subcellular organelle location to control early Ca2+ signal dynamics in vascular smooth muscle cells
10.1038/s41598-020-74700-x · 2020 · External reference
Current developments in Coot for macromolecular model building of Electron Cryo-microscopy and Crystallographic Data
10.1002/pro.3791 · 2020 · External reference
Lipid modifications of G proteins
10.1016/0955-0674(94)90139-2 · 1994 · External reference
Membrane-induced allosteric control of phospholipase C-β isozymes
10.1074/jbc.m114.586784 · 2014 · External reference
Molecular basis for Gβγ-mediated activation of phosphoinositide 3-kinase γ
10.1038/s41594-024-01265-y · 2024 · External reference
Structural and molecular characterization of a preferred protein interaction surface on G protein beta gamma subunits
10.1021/bi050655i · 2005 · External reference
Stimulation of phospholipase Cbeta by membrane interactions, interdomain movement, and G protein binding--how many ways can you activate an enzyme?
10.1016/j.cellsig.2007.04.006 · 2007 · External reference
Gβγ activates PIP2 hydrolysis by recruiting and orienting PLCβ on the membrane surface
10.1073/pnas.2301121120 · 2023 · External reference
Dissection of the steps of phospholipase C beta 2 activity that are enhanced by G beta gamma subunits
10.1021/bi0482607 · 2005 · External reference
Gq-initiated cardiomyocyte hypertrophy is mediated by phospholipase Cbeta1b
10.1096/fj.09-133983 · 2009 · External reference
Activation of phospholipase C β by Gβγ and Gαq involves C-Terminal rearrangement to release autoinhibition
10.1016/j.str.2020.04.012 · 2020 · External reference
Molecular basis for interactions of G protein betagamma subunits with effectors
10.1126/science.280.5367.1271 · 1998 · External reference
A2B adenosine receptor-triggered intracellular calcium mobilization: Cell type-dependent involvement of Gi, Gq, Gs proteins and protein kinase C
10.1007/s11302-025-10070-1 · 2025 · External reference
Purification of phospholipase C beta and phospholipase C epsilon from Sf9 cells
10.1385/1-59259-430-1:55 · 2004 · External reference
A dynamic model of membrane-bound phospholipase Cβ2 activation by Gβγ subunits
10.1124/mol.111.073403 · 2011 · External reference
Specificity and structural requirements of phospholipase C-beta stimulation by Rho GTPases versus G protein beta gamma dimers
10.1074/jbc.m208282200 · 2003 · External reference
Visualization of endogenous G proteins on endosomes and other organelles
10.7554/elife.97033.3 · 2024 · External reference
Crystal structure of Rac1 bound to its effector phospholipase C-beta2
10.1038/nsmb1175 · 2006 · External reference
A facile, sensitive and quantitative membrane-binding assay for proteins
10.1111/tra.12719 · 2020 · External reference
Mammalian phospholipase C
10.1146/annurev-physiol-030212-183750 · 2013 · External reference
Intrinsic Pleckstrin Homology (PH) domain motion in phospholipase C-β Exposes a Gβγ protein binding site
10.1074/jbc.m116.723940 · 2016 · External reference
Dissociation of the G protein βγ from the Gq-PLCβ complex partially attenuates PIP2 hydrolysis
10.1016/j.jbc.2021.100702 · 2021 · External reference
Purification of recombinant G proteins from Sf9 cells by hexahistidine tagging of associated subunits: characterization of alpha 12 and inhibition of adenylyl cyclase by alpha z
10.1074/jbc.270.4.1734 · 1995 · External reference
Purification, molecular cloning, and sequencing of phospholipase C-beta 4
10.1016/s0021-9258(19)36926-1 · 1993 · External reference
Activation of phospholipase C-β2 mutants by G protein αq and βγ subunits
10.1016/s0021-9258(19)74479-2 · 1993 · External reference
Regulation of phospholipase C-beta 4 by ribonucleotides and the alpha subunit of Gq
10.1016/s0021-9258(18)47252-3 · 1994 · External reference
Sites for Galpha binding on the G protein beta subunit overlap with sites for regulation of phospholipase Cbeta and adenylyl cyclase
10.1074/jbc.273.26.16265 · 1998 · External reference
An autoinhibitory helix in the C-terminal region of phospholipase C-β mediates Gαq activation
10.1038/nsmb.2095 · 2011 · External reference
Full-length Gα(q)-phospholipase C-β3 structure reveals interfaces of the C-terminal coiled-coil domain
10.1038/nsmb.2497 · 2013 · External reference
Structural insights into phospholipase C-β function
10.1124/mol.113.087403 · 2013 · External reference
G protein βγ subunits directly interact with and activate phospholipase Cϵ
10.1074/jbc.ra118.002354 · 2018 · External reference
A novel Gbetagamma-subunit inhibitor selectively modulates mu-opioid-dependent antinociception and attenuates acute morphine-induced antinociceptive tolerance and dependence
10.1523/jneurosci.2326-08.2008 · 2008 · External reference
Sites important for PLCbeta2 activation by the G protein betagamma subunit map to the sides of the beta propeller structure
10.1074/jbc.273.43.28298 · 1998 · External reference
UCSF Chimera--a visualization system for exploratory research and analysis
10.1002/jcc.20084 · 2004 · External reference
Heterotrimeric G protein subunit Gαq is a master switch for Gβγ-mediated calcium mobilization by Gi-Coupled GPCRs
10.1016/j.molcel.2020.10.027 · 2020 · External reference
cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination
10.1038/nmeth.4169 · 2017 · External reference
Structure of the phosphoinositide 3-kinase (PI3K) p110γ-p101 complex reveals molecular mechanism of GPCR activation
10.1126/sciadv.abj4282 · 2021 · External reference
Synergistic Ca2+ responses by G{alpha}i- and G{alpha}q-coupled G-protein-coupled receptors require a single PLC{beta} isoform that is sensitive to both G{beta}{gamma} and G{alpha}q
10.1074/jbc.m110.198200 · 2011 · External reference
CTFFIND4: Fast and accurate defocus estimation from electron micrographs
10.1016/j.jsb.2015.08.008 · 2015 · External reference
Phospholipase C beta2 association with phospholipid interfaces assessed by fluorescence resonance energy transfer: G protein betagamma subunit-mediated translocation is not required for enzyme activation
10.1074/jbc.271.41.25071 · 1996 · External reference
Membrane binding of phospholipases C-beta 1 and C-beta 2 is independent of phosphatidylinositol 4,5-bisphosphate and the alpha and beta gamma subunits of G proteins
10.1021/bi961606w · 1996 · External reference
Regulation of the rate and extent of phospholipase C beta 2 effector activation by the beta gamma subunits of heterotrimeric G proteins
10.1021/bi9811258 · 1998 · External reference
Coincident regulation of PLCβ Signaling by Gq-Coupled and μ-Opioid receptors opposes opioid-mediated antinociception
10.1124/molpharm.122.000541 · 2022 · External reference
Identification of a structural element in phospholipase C beta2 that interacts with G protein betagamma subunits
10.1074/jbc.273.12.7148 · 1998 · External reference
An integrated mechanism of Gq regulation of PLCβ enzymes
10.1073/pnas.2500318122 · 2025 · External reference
Exploring lipid-protein interactions in plant membranes
10.1093/jxb/erae199 · 2024 · External reference
Regulation of purified subtypes of phosphatidylinositol-specific phospholipase C beta by G protein alpha and beta gamma subunits
10.1016/s0021-9258(18)98401-2 · 1993 · External reference
G protein βγ subunits: central mediators of G protein-coupled receptor signaling
10.1007/s00018-008-8006-5 · 2008 · External reference
G-protein βγ subunits as multi-functional scaffolds and transducers in G-protein-coupled receptor signaling
10.1007/s00018-019-03275-2 · 2019 · External reference
Residue-wise local quality estimation for protein models from cryo-EM maps
10.1038/s41592-022-01574-4 · 2022 · External reference
Protein model refinement for cryo-EM maps using AlphaFold2 and the DAQ score
10.1107/s2059798322011676 · 2023 · External reference
Molecular dynamics flexible fitting: a practical guide to combine cryo-electron microscopy and X-ray crystallography
10.1016/j.ymeth.2009.04.005 · 2009 · External reference
Single vesicle fluorescence-bleaching assay for multi-parameter analysis of proteoliposomes by total internal reflection fluorescence microscopy
10.1021/acsami.2c07454 · 2022 · External reference
Molecular cloning, expression and regulatory activity of G alpha 11- and beta gamma-subunit-stimulated phospholipase C-beta from avian erythrocytes
10.1042/bj3160559 · 1996 · External reference
Kinetic scaffolding mediated by a phospholipase C-beta and Gq signaling complex
10.1126/science.1193438 · 2010 · External reference
The structure of the G protein heterotrimer Giα1β1γ2
10.1016/0092-8674(95)90220-1 · 1995 · External reference
The pleckstrin homology domain of phospholipase C-beta(2) links the binding of gbetagamma to activation of the catalytic core
10.1074/jbc.275.11.7466 · 2000 · External reference
Genetic alteration of phospholipase C beta3 expression modulates behavioral and cellular responses to mu opioids
10.1073/pnas.96.18.10385 · 1999 · External reference
A simple guide to biochemical approaches for analyzing protein-lipid interactions
10.1091/mbc.e11-07-0645 · 2012 · External reference
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy
10.1038/nmeth.4193 · 2017 · External reference
Gβγ engages PLCβ3 at multiple sites to reorient and facilitate its activation
10.4231/ek7t-rt26 · 2026 · External reference
Gb1g2 crosslinked to PLCb3
10.2210/pdb9y7h/pdb · 2026 · External reference
Gbg crosslinked to PLCb3 - second conformation
10.2210/pdb9yao/pdb · 2026 · External reference
Gbg crosslinked to PLCb3 - second conformation
10.2210/pdb9yap/pdb · 2026 · External reference
Gb1g2 crosslinked to PLCb3
2026 · External reference
Gbg crosslinked to PLCb3 - second conformation
2026 · External reference
Gbg crosslinked to PLCb3 - second conformation
2026 · External reference
