Abstract
ABSTRACT
This study aimed to identify bioactive constituents of Jingtong Granules (JTG), a traditional Chinese medicine for cervical spondylotic radiculopathy (CSR), characterize their in vivo exposure, and explore pharmacological targets and pathways. Chemical constituents were characterized by LC–MS with GNPS‐based molecular networking, and key compounds were prioritized via molecular docking; pharmacokinetic analysis was characterized in vivo exposure, and anti‐inflammatory activity was evaluated via NO production in LPS‐stimulated BV2 cells. Network pharmacology with GO and KEGG enrichment identified potential targets and pathways. Seventy‐three components were identified in vitro and 10 in vivo. Albiflorin, puerarin, and ginsenoside Rg1 were rapidly absorbed and reduced NO production dose‐dependently. Network analysis suggested MMP9 and PTGS2 as key targets, involving TNF and PI3K‐AKT signaling pathways. JTG and its exposed constituents exert anti‐inflammatory effects by modulating inflammatory targets and pathways. Albiflorin, puerarin, and ginsenoside Rg1 are prioritized as candidate bioactive constituents; MMP9, PTGS2, TNF, and PI3K‐AKT pathways are putative mechanistic nodes requiring further validation.