Abstract
ABSTRACT
Precisely integrating MOF powders into patterned electrochemical devices remains a processing challenge, as conventional methods often require masks, manual alignment, or poorly scalable steps and can lead to non‐selective deposition and inefficient material use. While electrophoretic and dielectrophoretic deposition methods for particulate materials and GOx@ZIF‐8/Prussian Blue (PB) glucose biosensors have been reported independently, low‐volume localization of enzyme‐loaded MOFs directly onto active microelectrode transducer regions remains challenging. Here, we develop a mask‐free, DC‐field‐assisted microdroplet strategy that selectively localizes GOx@ZIF‐8 onto PB‐modified interdigitated electrodes (PB@IDEs). The approach uses only 12 µL of GOx@ZIF‐8 suspension and, unlike random drop‐casting, preferentially confines the biocatalytic MOF to the PB transduction interface, minimizing inactive deposition and improving enzyme utilization. The resulting Nafion/GOx@ZIF‐8/PB@IDE biosensor exhibits reproducible spatial localization and approximately threefold higher sensitivity, together with a lower limit of detection, than an otherwise identical drop‐cast electrode. The sensor shows a linear amperometric response over 24–175 µM glucose concentration, overlapping concentrations relevant to sweat glucose analysis. These results demonstrate that electro‐addressable placement of enzyme@MOF particles can improve reaction‐readout coupling while reducing biocatalyst consumption.