Analysis of aflatoxins in various food and feed matrices-results of international validation studies

Collaborative studies were conducted to evaluate the effectiveness of analytical methods based on immunoaffinity column clean-up prior to high performance liquid chromatography (HPLC) for the determination of aflatoxin B1 and total aflatoxins at European regulatory limits in various food products (4 ng/g total aflatoxins, 2 ng/g aflatoxin B1), baby food (0.1 ng/g aflatoxin B1 in discussion) and feeding stuff (1 ng/g aflatoxin B1 in discussion).The methods showed acceptable within-laboratory and between-laboratory precision for all matrices at these low levels for the determination of both, total aflatoxins and aflatoxin B1.