Abstract
Herein, we designed a dual DNP-caged near-infrared probe, N-Bu-DCIB-2DNP, by conjugating dicyanoisophorone (DCI) with an N-alkylated benzimidazole (B) through double vinylene linkers. This probe features two responsive sites-the DNP ether on the donor and the DNP-functionalized electron-deficient quaternary carbon on the DCI unit-to enable a cascade thiolysis strategy. The probe exhibits distinct dual-emission fluorescence signals at 610 nm and 745 nm with large Stokes shifts, and achieves a rapid response to H2S within 6 min, with a detection limit as low as 51.48 nM. Furthermore, test strips loaded with N-Bu-DCIB-2DNP enable convenient visual detection of H2S in spoiled food samples, and its applicability in real water samples has also been validated, demonstrating its promising practical utility.