Abstract
Objective
To explore effects of cerebral ischemia on the ceruloplasmin (Cp) expression in the cortex and hippocampus of rats.Methods
Male Wistar rats were randomly divided into cerebral ischemia group and control group. Cerebral ischemia was induced by ligating bilateral common carotid arteries and the ischemic rats were further subgrouped according to ischemia time. The control rats received a sham operation. The expression of Cp mRNA in the cortex and hippocampus was measured by reverse transcription polymerase chain reaction (RT-PCR). The Cp expression was shown by immunohistochemistrical (streptavidin peroxidase, SP) method.Results
In ischemia group, the expression of Cp mRNA in the cortex and hippocampus decreased compared with that in control group (PConclusion
Cerebral ischemia inhibited Cp expression in the cortex and hippocampus of rats. The decrease of Cp might be involved in iron deposition in neurons.