Abstract
The Protein Secondary Structure Visualizer (ProS2Vi) is a novel Python-based visualization tool designed to enhance the analysis and information accessibility of protein secondary structures, calculated and identified using the Dictionary of Secondary Structure of Proteins (DSSP) algorithm. Leveraging robust Python libraries such as "Biopython" for data handling, "Flask" for Graphical User Interface (GUI), "Jinja2", and "wkhtmltopdf" for visualization, ProS2Vi offers a modern and intuitive representation for visualization of the DSSP assigned secondary structures to each residue of any proteins' amino acid sequence. Significant features of ProS2Vi include customizable icon colors, the number of residues per line, and the ability to export visualizations as scalable PDFs, enhancing both visual appeal and functional versatility through a user-friendly GUI. We have designed ProS2Vi specifically for secure and local operation, which significantly increases security when working with novel protein data.