Abstract
Wheat bran, a significant cereal by-product, is widely used in animal husbandry and the food industry. Our previous study identified a correlation between fiber utilization in Tibetan pigs and their efficient hindgut fermentation capacity. In this study, the Bacillus altitudinis strain Z-99, capable of cellulose degradation, was identified and isolated from Tibetan pigs. The results of solid-state fermentation demonstrated a significant reduction in neutral detergent fiber (NDF), acid detergent fiber (ADF), and hemicellulose content by 12.26 % (P P P P Bacillus altitudinis strain Z-99 enhanced the in vitro fermentation characteristics of wheat bran. In particular, it significantly increased the theoretical maximum gas production (P P P P P P Bacillus altitudinis strain Z-99 significantly increased the abundance of Bifidobacterium (P Blautia (P Enterococcus (P Actinobacillus (P Bacillus altitudinis strain Z-99 with cellulose-degrading capacity was isolated from Tibetan pigs, and its functionality was validated through solid-state fermentation and in vitro fermentation methods. This study provided valuable insights into the utilization of wheat bran and the exploration of cellulose-degrading bacteria.