Abstract
Green algae have a great potential as biofactories for the production of proteins. Chlamydomonas reinhardtii, a representative of eukaryotic microalgae, has been extensively used as a model organism to study light‐induced gene expression, chloroplast biogenesis, photosynthesis, light perception, cell–cell recognition, and cell cycle control. However, little is known about the glycosylation machinery and N‐linked glycan structures of green algae. In this study, we performed mass spectrometry analysis of N‐linked oligosaccharides released from total extracts of Chlamydomonas reinhardtii and demonstrated that C. reinhardtii algae possess glycoproteins with mammalian‐like sialylated N‐linked oligosaccharides. These findings suggest that C. reinhardtii may be an attractive system for expression of target proteins.