Abstract
The present work aimed to study the intensity of electron spin resonance (ESR) of hemoglobin rat's polymer and changes in rheological properties (viscosity). This study included five groups: control, irradiation with single dose of 5 Gy gamma irradiation, intraperitoneal injection with single dose of arsenic trioxide (As2O3) 10 mg/kg body weight, As2O3 followed by 5 Gy and 5 Gy followed by As2O3. The results of ESR spectroscopy indicated that the intensity increases significantly for As2O3 and non-significantly with 5 Gy of gamma radiation compared with control, which indicated an increase in the number of free radicals. Injection with As2O3 after and before 5 Gy resulted in non-significant decrease which given rise to a decrease in the number of free radicals. there was significant decrease in viscosity for 5 Gy at different shear rates (11.3–375 sec−1), viscosity for As2O3 injection group showed non-significant decrease, and non-significant increase in case of 5 Gy followed by As2O3 injection, significant decrease at high shear rate in case of 5 Gy followed by As2O3. These results concluded that 5 Gy followed by As2O3 showed some sort of repair in the function of rats hemoglobin rather than injection with As2O3 and 5 Gy both individually. Keywords: Gamma radiation, Arsenic trioxide, Viscosity, Electron spins resonance