Abstract
For the first time, this report confirmed under real-laboratory-contamination scenarios that ozone disinfection effectively reduces microbial nucleic acid background in mNGS laboratories, with consistent and efficient disinfection performance. With the global advancement of standardized clinical application of mNGS technology, the results provide a practical strategy to control laboratory contamination, reduce false-positive risks, and maintain a low nucleic acid baseline. This method addresses the difficulty of eliminating persistent contamination in mNGS laboratories and offers a direct reference for environmental decontamination of metagenomic sequencing laboratories worldwide.