Antibacterial effect of silver nanofluid synthesized using herbal medicine on Pseudomonas aeruginosa and Klebsiella pneumoniae

The antibacterial property of silver nanofluid was investigated on Pseudomonas aeruginosa and Klebsiella pneumoniae. Silver nanoparticles were synthesized using Nigella sativa (black seeds), green tea, ginger, and garlic as reductant agents through hydrothermal and microwave methods. The physical characteristics of nanoparticles were examined by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), Energy-dispersive X-ray spectroscopy (EDX), and visible ultraviolet spectroscopy (UV–vis). The crystal size measured from SEM analysis on average was about 60 nm and calculated from Debey-Scherer equation was around 43 nm. The nanofluid was prepared by solving the nanoparticles in deionized water. The effect of prepared nanofluid was examined on the growth of two kinds of bacteria that is Pseudomonas aeruginosa and Klebsiella pneumoniae by an antibacterial sensitivity test using the pour plate method. The nanofluids showed a considerable antibacterial effect on Pseudomonas aeruginosa and a moderate effect on Klebsiella pneumoniae on agar plates.