Open the manuscript using the link above. DOC files open in Word or a compatible document reader.
Abstract
We describe the synthesis and characterization of melanin-like nanoparticles (MNP) as novel contrast agents for optoacoustic tomography. Good dispersion stability of high concentration MNPs in different biological media was achieved with thiol-terminated methoxy-poly(ethylene glycol), which can be used for further functional conjugation. MNP-PEG were found biocompatible with human MCF-7 and 3T3 cells. Cell toxicity of MNPs was found lower than that of gold nanorods for concentrations that provide equal optical absorbance. Optoacoustic tomography images were obtained with Laser Optoacoustic Imaging System (LOIS-3D) from tubes filled with contrast agents and live mice. Imaging of tubes permitted verification of the system resolution <300 μm and sensitivity Δμa=0.03/cm under safe laser fluence of 20 mJ/cm(2). Water suspensions of MNP demonstrated optoacoustic efficiency that is about equal to that of gold nanorods under conditions of equal optical absorption. We conclude that MNPs have the potential for biomedical imaging applications as optoacoustic contrast agents.
Multiscale photoacoustic microscopy and computed tomography
10.1038/nphoton.2009.157 · 2009
Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo
10.1109/tmi.2004.843199 · 2005
Laser Optoacoustic tomography: Towards new technology for biomedical diagnostics
10.1016/j.nima.2012.12.035 · 2013
Biomedical photoacoustic imaging
10.1098/rsfs.2011.0028 · 2011
Prospects of photoacoustic tomography
10.1118/1.3013698 · 2008
Optical properties of circulating human blood in the wavelength range 400–2500nm
10.1117/1.429919 · 1999
Optical and opto-acoustic imaging
10.1007/978-3-642-10853-2_4 · 2013
Gold and silver nanoparticles as contrast agents for optoacoustic imaging. Chapter 30
2009
unpaywall
datacite
Nanoparticles as optoacoustic contrast agents for optoacoustic imaging. Chapter 5
2015
Quantitative photoacoustic measurement of tissue optical absorption spectrum aided by an optical contrast agent
10.1364/oe.17.004879 · 2009
A theoretical and experimental study of light absorption and scattering by in vivo skin
10.1088/0031-9155/25/4/008 · 1980
The red and the black
10.1021/ar100079y · 2010
Current understanding of the binding sites, capacity, affinity and biological significance of metals in melanin
10.1021/jp071439h · 2007
Chemistry of mixed melanogenesis- pivotal role of dopaquinone
10.1111/j.1751-1097.2007.00238.x · 2008
Vaccinia virus-mediated melanin production allows MR and optoacoustic deep tissue imaging and laser-induced thermotherapy of cancer
10.1073/pnas.1216916110 · 2013
Detection of melanoma metastases in resected human lymph nodes by noninvasive multispectral photoacoustic imaging
10.1155/2014/163652 · 2014
Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy
10.1117/1.2210907 · 2006
Photoacoustic microscopy of tyrosinase reporter gene in vivo
10.1117/1.3606568 · 2011
Mulitwavelength photoacoustic imaging of inducible tyrosinase reporter gene expression in xenograft tumors
10.1038/srep05329 · 2014
Tyrosinase as a multifunctional reporter gene for photoacoustic/MRI/PET triple modality molecular imaging
10.1038/srep01490 · 2013
The inherent cytotoxicity of melanin precursors: a revision
10.1016/0167-4889(94)90250-x · 1994
Melanocytes as instigators and victims of oxidative stress
10.1038/jid.2014.65 · 2014
Control of In Vivo transport and toxicity of nanoparticles by tea melanin
10.1155/2012/746960 · 2012
Melanin-covered nanoparticles for protection of bone marrow during radiation therapy of cancer
10.1016/j.ijrobp.2010.02.020 · 2010
Water-soluble ultra-small melanin nanoparticles as a naturally active platform for multimodality imaging
2014
Bioinspired polymerization of dopamine to generate melanin-like nanoparticles having an excellent free-radical scavenging property
10.1021/bm101281b · 2011
Spontaneous autoxidation of dopamine
1995
Isolation and biophysical studies of natural eumelanins: applications of imaging technologies and ultrafast spectroscopy
10.1046/j.1600-0749.2003.00098.x · 2003
The synthetic melanin nanoparticles having an excellent binding capacity of heavy metal ions Bull Korean
2012
Bio-inspired, melanin-like nanoparticles as a highly efficient contrast agent for T1-weighted magnetic resonance imaging
10.1021/bm4008138 · 2013
Highly purified biocompatible gold nanorods for contrasted optoacoustic imaging of small animal models
10.1166/nnl.2012.1387 · 2012
Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research
10.4172/2157-7439.s2-001 · 2012
Apoptosis, Cytotoxicity and Cell Proliferation, Roche Diagnostics GmbH
2008
Whole body three-dimensional optoacoustic tomography system for small animals
10.1117/1.3259361 · 2009
Three-dimensional optoacoustic imaging as a new noninvasive technique to study long-term biodistribution of optical contrast agents in small animal models
10.1117/1.jbo.17.10.101506 · 2012
Laser Optoacoustic Tomography: Towards New Technology for Biomedical Diagnostics
10.1016/j.nima.2012.12.035 · 2013
Enabling in vivo measurements of nanoparticles concentrations with three-dimensional optoacoustic tomography
10.1002/jbio.201200233 · 2014
Additional external references
Laser nanothermolysis of human leukemia cells using functionalized plasmonic nanoparticles
10.5101/nbe.v4i2.p66-75 · doi-reference
Plasmon resonance in ellipsoid nanoparticles
10.1070/qe2002v032n01abeh002131 · doi-reference
Half-time image reconstruction in thermoacoustic tomography
10.1109/tmi.2004.839682 · doi-reference
An imaging model incorporating ultrasonic transducer properties for three-dimensional optoacoustic tomography
