Investigations into the role of rats as intermediate hosts for Neospora caninum in Chennai, India

Attempts were made to detect Neospora caninum in rats (Rattus rattus) in and around Chennai, India. During the study, 112 feral rats were trapped and blood, brain, heart, lungs and diaphragm samples were collected for serological, parasitological and molecular identification of N. caninum. Out of 112 rats, cyst-like structures were identified in 16 brain squash samples. However, the identity of the cysts could not be confirmed as N. caninum. A total of 12 sera samples were positive for N. caninum by indirect fluorescence antibody test (IFAT). Four of the 'cyst' positive samples were also positive by IFAT. None of the above samples showed amplification of N. caninum (Nc5) or toxoplasmatiid (ITS-1) fragments by PCR. In conclusion, the present study showed 10.71% seroprevalence of N. caninum among feral rats, which is a first report in India. Low prevalence of the organism in the environment and the consequent low chance of exposure of rats to N. caninum might explain the failure to detect the DNA in any of the samples tested in the study.