Abstract
Background
Cross-infection may occur if alginate impressions are not disinfected. Available chlorine (AC), though common, poses safety risks. Chlorogenic acid (CA), a natural compound, shows effective disinfection and, as we previously verified, inhibits pathogens without damaging impressions.Objective
This study investigates the influence of CA and AC on the oral microbial community of alginate impressions from a community-ecology perspective.Methods
The maxillary impressions of 60 participants were randomly assigned to four groups (n = 15). The NS-AC group received a normal saline (NS) rinse and a 2,000 mg/L AC spray. The NS-CA group got an NS rinse and a CA (10 mg/mL mix + 60 mg/mL spray). The CA-AC group obtained a 10 mg/mL CA rinse and a 2,000 mg/L AC spray. The CA-CA group acquired a CA (10 mg/mL rinse & mix + 60 mg/mL spray). An analysis of 16S rRNA sequencing was conducted on the samples that were collected.Results
The NS-CA group exhibited substantially higher Chao1, Shannon and Simpson indices than the NS-AC group (p Neisseria, Porphyromonas and Prevotella were identified through LEfSe analysis and a random forest model (LDA > 4, AUC = 0.911). A synchronous decrease in the NS-CA was predicted by FAPROTAX functional prediction in relation to aromatic_compound_degradation and human_pathogens_all. The NS-CA and CA-CA did not exhibit any significant differences in alpha diversity or FAPROTAX (p > 0.05), and the PCoA showed that the two groups were closely clustered.Conclusion
The stabilization and maturation of oral microbiota on the surface of alginate impressions are facilitated by CA disinfection. Of all the groups, the NS-CA group demonstrates specific cost-effectiveness advantages and has the potential to serve as an alternative to conventional AC disinfection.