Antioxidant activity, total phenolics, flavonoids and antinutritional characteristics of germinated foxtail millet (Setaria italica)

A central composite rotatable design was applied to analyse the effects of independent variables [soaking time (ST), germination time (Gt) and temperature (GT)] on responses [antioxidant activity (AoxA), total phenolic contents (TPC) and flavonoid contents (TFC)]. The results indicated that with increase in ST, Gt and GT, AoxA, TPC (free/bound) and TFC (free/bound) of foxtail millet increased significantly. The best combination of germination bioprocess variables for producing optimized germinated foxtail millet flour with the highest AoxA (90.5%), TPC (45.67 mg gallic acid equivalent (GAE)/100 g sample) and TFC (30.52–43.96 mg RU/g sample) were found with soaking time of 15.84 min having germination temperature of 25°C. The optimized germinated foxtail millet flour was nutritionally rich as it produced higher protein (14.32 g/100 g), dietary fibre (27.42 g/100 g), calcium (25.62 mg/kg), iron (54.23 mg/kg), magnesium (107.16 mg/kg) and sodium (69.45 mg/kg) per kg as compared to un-germinated foxtail millet flour.