Abstract
Two Gram-stain-negative, rod-shaped, aerobic bacterial strains, designated 200-1SW
T
and C21-150M6
T
, were isolated from seawater samples of the western Pacific Ocean. Both strains were able to grow in the presence of 1–10% NaCl, at pH 6–9 and at 10–37 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that both strains belong to the genus
Pseudooceanicola
, with strain 200-1SW
T
closest to
Pseudooceanicola marinus
CECT 7751
T
(97.6% sequence similarity) and C21-150M6
T
to
Pseudooceanicola flagellatus
DY470
T
(97.8%). Genome sizes were 4.77 Mb (G+C content: 67.1 mol%) for 200-1SW
T
and 4.32 Mb (G+C content: 63.4 mol%) for C21-150M6
T
, with average nucleotide identity and digital DNA–DNA hybridization values with other
Pseudooceanicola
species below 86.5 and 32.0%, respectively.
In silico
genome mining identified several putative secondary metabolite biosynthetic gene clusters, suggesting that the strain possesses unexplored biosynthetic potential. Cellular fatty acid profiles identified summed feature 8 (C
18 : 1
ω
7
c
and/or C
18 : 1
ω
6
c
), C
19 : 0
cyclo
ω
8
c
and C
16 : 0
as the predominant fatty acids for 200-1SW
T
, as well as C
18 : 1
ω
7
c
11-methyl, C
16 : 0
, C
18 : 0
and summed feature 8 as the major fatty acids for C21-150M6
T
. The only respiratory quinone detected was ubiquinone 10 for both strains. The major polar lipids of both strains were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine. Based on phenotypic, chemotaxonomic and genomic characteristics, strains 200-1SW
T
and C21-150M6
T
represent two novel species within the genus
Pseudooceanicola
, for which the names
Pseudooceanicola aquaeponti
sp. nov. and
Pseudooceanicola profundi
sp. nov. are proposed; the type strains are 200-1SW
T
(=MCCC 1A12849
T
=NBRC 117448
T
) and C21-150M6
T
(=MCCC M22851
T
=NBRC 117449
T
), respectively.