Abstract
Anomalies of meiosis frequently result in abnormal chromosome segregation, which leads to defects in pollen formation in maize. Thus, assessing pollen viability is an important measure for examining the overall success of male sexual reproduction. Pollen viability tests are used to characterize mutants defective in meiosis and microsporogenesis, and to determine the effects of genome instability and environmental conditions on reproduction. This protocol describes a rapid method for assessing pollen viability in maize, using a simplified cytological staining approach. Traditional pollen staining methods, such as Alexander staining, often require hazardous chemicals that are increasingly restricted due to safety concerns. The method presented here uses easily accessible reagents and avoids highly toxic substances.