Abstract
ABSTRACT
Aim
The myogenic response contributes considerably to blood flow autoregulation. Intracellular pH (pH
i
) has a substantial impact on small artery contractility. However, the role of putative pH
i
changes during myogenic constriction is unknown. Therefore, the hypothesis was tested that changes in pH
i
are associated with and contribute to the myogenic response in small arteries.
Methods
Rat small tail arteries were studied using isobaric myography and BCECF‐ as well as FURA‐2‐fluorimetry.
Results
We found that in response to a pressure increase, pH
i
decreased. It initially reached a peak and then recovered partly. The initial pH
i
peak is augmented in bicarbonate‐free solution, but was not affected by inhibitors of Na
+
/H
+
exchange. The pH
i
recovery was not altered in bicarbonate‐free solution, but was reduced by inhibitors of Na
+
/H
+
exchange. Proton fluxes were larger at 80 mmHg than at 10 mmHg. Bicarbonate‐free solution had no effect on proton fluxes at either 80 mmHg or 10 mmHg. Inhibitors of Na
+
/H
+
exchange reduced proton fluxes only at 80 mmHg. Pressure‐induced changes in intracellular calcium were not affected by either bicarbonate‐free solution or inhibitors of Na
+
/H
+
exchange.
Conclusions
This study showed that in rat small tail arteries a pressure increase is accompanied by a decrease in pH
i
that appears to facilitate the myogenic response. The results of this study suggest that the pressure‐induced immediate fall in pH
i
is caused by a release of protons from the contractile machinery, and that the subsequent pH
i
recovery is due to activation of Na
+
/H
+
exchange.