Abstract
Abstract
Background and Objectives
To assess the effects of long‐term regular leukocyte‐reduced platelet (PLT) apheresis on lymphocyte subsets in volunteer donors.
Materials and Methods
We enrolled 178 participants, including Amicus‐using donors (Group A,
n
= 55), Trima‐using donors (Group T,
n
= 64), and first‐time donor controls (
n
= 59). Apheresis donors completed ≥8 PLT collections within 1 year on one fixed separator. Lymphocyte‐subset measurements were performed on pre‐donation screening samples.
Results
After age/sex adjustment, both apheresis groups exhibited significantly lower absolute counts and percentages of natural killer (NK) cells versus controls (
p
+
, CD4
+
, CD8
+
T cells, and B lymphocytes were similar among groups. Several T‐cell markers showed nominal downward trends with higher lifetime donations (raw
p
Conclusion
Long‐term leukocyte‐reduced PLT apheresis is associated with decreased circulating NK cells. Multiple‐testing correction provided no evidence for progressive lymphocyte decline with cumulative donations. One‐year donation frequency had no impact on lymphocyte subsets. With plasma rinseback activated for all Trima procedures, lymphocyte profiles were comparable between devices. Routine hematological parameters remained stable in long‐term apheresis donors.