Abstract
Background
Chronic airway dysbiosis plays an important role in the pathogenesis of cystic fibrosis (CF) lung disease and may serve as a therapeutic target. However, studies investigating the effects of direct therapeutic targeting of the airway microbiome are lacking. In this study, we therefore used βENaC-overexpressing (βENaC-Tg) mice and determined the evolution of abnormal lung microbiota and effects of re-balancing bacterial communities on chronic airway inflammation and mucus plugging in this model of CF lung disease.
Methods
The development of the respiratory microbiome was determined by 16S rRNA gene sequencing and the effects of preventive intranasal instillation of endogenous probiotic bacteria on the lung phenotype were determined in βENaC-Tg mice and wild-type littermates.
Results
Neonatal βENaC-Tg mice developed severe respiratory dysbiosis characterized by an increase in the relative abundance of
Streptococcus
and a decrease in
Ligilactobacillus
compared to wild-type littermates.
Ligilactobacillus murinus
SMH17 was identified as the dominant
Ligilactobacillus
species in the lungs of neonatal wild-type mice. Preventive treatment by intranasal instillation of
L. murinus
SMH17 was well tolerated and reduced age-specific markers of airway inflammation including inflammatory cell counts and proinflammatory cytokines in neonatal and juvenile βENaC-Tg mice. In addition, preventive treatment with
L. murinus
SMH17 reduced airway mucus plugging in βENaC-Tg mice by ∼40%.
Conclusion
Preventive intrapulmonary application of the endogenous probiotic
L. murinus
SMH17 reduces airway inflammation and mucus plugging in mice with CF-like lung disease. This data supports further elucidation of inhaled probiotics as a strategy to treat chronic airway dysbiosis in patients with CF.