Abstract
PBMC from HLA-A2 positive patients are isolated from the peripheral blood by Leucapheresis. Monocytes are seperated by adherence and differentiated into immature DC by incubation with GM-CSF and IL-4 under serumfree conditions over six days. Then DC are matured with TNF-α and PGE 2 and pulsed with three different PSA-Peptides (PSA-1 [FLTPKKLQCV]; PSA-2 [KLQCVDLHV]; PSA-3 [VISNDVCAQV]). For vaccination 6 × 10 Peptide-pulsed DC are applied intracutaneously after previous subcutaneous application of INF-γ (50 μg/m). Vaccination is performed 4 times in 3 week intervals. Patients are included after informed consent and sufficient hematological liver and renal function. Primary end point of the trial is the evaluation of PSA, measurable tumour parameter after 4 vaccinations and clinical benefit. Secondary end points are safety, quality of life (EORTC-QLQ-C30) and immunological parameters. Until now 8 patients are enrolled. 4 patients completed all 4 scheduled vaccination. 3 patients are still under vaccination. One patient dropped out due to early progress. The vaccination is well tolerated without any therapy related adverse event. Response rates are under evaluation and will be presented. Vaccination with PSA-peptide-pulsed autologous DC combined with INF-γ is feasible and well tolerated. Further evaluation is in progress.