Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels | ResearchHub
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Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels
Vincenzo Izzo, Maria Assunta Costa, Renata Di Fiore, Giovanni Duro, Daniele Bellavia, Eleonora Cascone, Paolo Colombo, Maria Concetta Gioviale, Rainer Barbieri
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Abstract
Abstract
An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularirly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms.
