Abstract
Abstract
The protein Talin1 encoded by the
TLN1
gene is a focal adhesion-related protein that binds to various cytoskeletal proteins and plays an important role in cell adhesion and movement. Recent studies have shown that it is overexpressed in prostate cancer, liver cancer, and oral squamous cell carcinoma, and is closely related to tumor progression and metastasis. This study integrated bioinformatics and functional analysis to reveal the prognosis and potential functions of
TLN1
in AML. The results showed that the expression level of
TLN1
was abnormally increased in AML and localized in the cell membrane and cytoplasm, and
TLN1
is a significant prognostic indicator of overall survival (OS). Enrichment analysis of related genes showed that
TLN1
is related to neutrophil mediated immunity, neutrophil activation and may regulate important signal pathways in hematological tumors including tyrosine kinase receptor, FLT3 and PIK3/AKT. The PPI network shows that
TLN1
and
MYH9
may be involved in the process of AML tumors together with
PIP5K1C, ROCK1, S100A4, MY01A
and
WAC
. Immune infiltration analysis explains that
TLN1
is associated with multiple immune cells and may be an important immune marker in AML. Furthermore, molecular biology experiments confirmed that
TLN1
is related to the proliferation, differentiation and cycle of AML cells. Silencing
TLN1
can inhibit the proliferation of AML cells and promote differentiation through the Talin1/P-AKT/CREB signaling pathway.