Abstract
Abstract
Objectives
To explore whether transmembrane 4 superfamily 1 (TM4SF1) can modulate the transforming growth factor β1 (TGF-β1)/mothers against decapentaplegic homolog 3 (Smad3) signaling pathway to influence nasopharyngeal carcinoma (NPC) cells from the aspect of malignant biological activity and autophagy.
Methods
Reverse transcriptase-polymerase chain reaction was utilized for measuring the relative expression of TM4SF1 in human nasopharyngeal epithelial cell line (NP69) as well as human NPC cells (5–8F, CNE2, and 6–10B). A small interfering-negative control (si-NC) group, a si-TM4SF1 group, a si-TM4SF1 + TGF-β1 group, and a control group (not transfected with any plasmid) were established for the assignment of 5–8F cells in a random manner. Cell proliferation, apoptosis, autophagy, and protein expression were evaluated by Cell Counting Kit-8 assay, flow cytometry, monodansylcadaverine (MDC) staining/transmission electron microscopy (TEM), and Western blotting, respectively.
Results
We found that TM4SF1 expression was higher in human NPC 5–8F, CNE2, and 6–10B cells than in NP69 cells, and its relative expression significantly rose in 5–8F cells compared with 6–10B and CNE2 cells (p
Conclusions
TM4SF1 knockdown is able to influence NPC cells by repressing their proliferation and infiltration while inducing their apoptosis.