Abstract
Abstract
Objectives
Acute kidney injury (AKI) is a serious clinical condition characterized by rapid renal dysfunction and high mortality. Although the dysregulation of microRNAs is well-established in AKI, the clinical significance of microRNA-200c in this condition remains poorly defined.
Methods
RT-qPCR detected miR-200c and inflammatory factors (TNF-α, IL-6, IL-8) in serum from 75 patients with AKI and 72 controls. ROC curves assessed miR-200c’s diagnostic value. Multivariate Cox regression identified independent survival risk factors. Kaplan-Meier curves analyzed miR-200c-survival links. Establishment of an
in vitro
cell model induced by LPS. To assess cell proliferation and apoptosis, CCK-8 assays and flow cytometry were employed.
Results
miR-200c expression was significantly elevated in AKI patients. Serum miR-200c effectively distinguishes AKI patients from controls. Compared with the group of patients with good prognosis, the miR-200c expression was significantly higher in the group of patients with poor prognosis. Survival analysis showed that higher miR-200c levels in AKI cases correlated with a notable decline in 90-day survival. Suppression of miR-200c expression enhanced cell proliferation, attenuated apoptosis, and reduced inflammatory factor levels, thereby ameliorating AKI progression.
Conclusions
miR-200c shows promising application potential in the diagnosis and prognosis assessment of AKI. Suppressing miR-200c expression effectively curbs aberrant cellular behaviors and pro-inflammatory responses.