Abstract
Abstract
Objectives
Exercise and cognitive rehabilitation exercises (EC) have been shown to slow the progression of Alzheimer’s disease (AD). To evaluate the regulation of EC for the miR-103a-3p/BDNF expression to delay the progression of AD.
Methods
AD patients were assigned to control (n=30) and EC groups (n=30) using a random number table method. Cognition was assessed using the Mini-Mental State Examination (MMSE), Activity of Daily Living (ADL), and the Alzheimer’s Disease Assessment Scale–Cognition (ADAS-Cog) before and after treatment.in AD patients. The expression of miR-103a-3p in AD patients was detected by the RT-qPCR method. The expression of amyloid β-protein (Aβ), phosphorylated Tau proteins (p-Tau), BDNF, tumor necrosis factor-α (TNF-α), and interleukin-1 beta (IL-1β) in the serum of AD patients was measured by ELISA.
Results
After six months of EC treatment for them, MMSE and ALD scores were elevated, and ADAS-cog scores were reduced in AD patients. In addition, serum Aβ
1-42
was elevated and p-Tau was decreased in AD patients after EC treatment. RT-qPCR results indicated that the expression of miR-103a-3p was reduced in AD patients after EC treatment. The results of ELISA indicated that the expression of BDNF was increased while TNF-α and IL-1β were decreased in AD patients after EC treatment. Dual-luciferase reporter assay confirmed miR-103a-3p targeting of BDNF.
Conclusions
EC may affect miR-103a-3p/BDNF expression to inhibit the expression of pro-inflammatory factors (TNF-α and IL-1β), thus alleviating AD.