Construction of a eukaryotic expression plasmid of Humanin

Objective To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.Methods The recombinant plasmid pGEMEX-1-Humanin was digested with restriction endonucleases BamH I and Hind III and the Humanin gene fragments, about 100 bp length, were obtained. Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-) and the recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.Results Recombinant plasmid DNA successfully produced a band which had the same size as that of the Humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence.Conclusions A eukaryotic expression plasmid of Humanin was successfully constructed.