Abstract
Collagen peptides produced by milkfish (Chanos chanos) scales can be an alternative to replace collagen from mammals. Besides the high price of mammalian collagen, it also risks spreading infectious diseases like Bovine Spongiform Encephalopathy (BSE) and Transmissible spongiform encephalopathies (TSEs). This study aims to identify the effect of acetic acid and pepsin concentrations in optimizing the extraction of collagen peptides from milkfish scales due to waste and their application as a bioactive emulsifier. This study used an experimental design using a completely randomized design (CRD) with a 3 x 2 factorial pattern with three replications. The first factor is three levels of acetic acid concentration, and the second is three levels of pepsin concentration. The results of this study on the proximate analysis of collagen peptides showed that the results of the moisture content test, ash content test, protein content test, fat content test, and pH test met the requirements for collagen quality.