Abstract
Rising demand fornatural colorants,coupled with health concerns over synthetic dyessuch as Red K3 and Red K10, motivates the development of plant-based alternatives for cosmetics applications. Here, we formulated lipstick incorporating anthocyanins extracted from Binahong (Anredera cordifolia) fruitsusing acidified water (0.01% HCl, v/v) and purified viaAmberlite XAD-7 HP resin.Refined Binahong fruit extract (RBFE) was analyzed byUV-Vis spectrophotometryand LC-MS, whichclassified its anthocyanins as C-type based on absorbance patterns at pH 1 and 4.5. This indicating an acyl groups on the B-ring. Four anthocyaninswere identified: pelargonidin-3-O-glucoside (m/z 433), pelargonidin-3-O-(6”-malonyl) glucoside (m/z 519), petunidin-3-O-(6”-malonyl) glucoside (m/z 565), and cyanidin-3-O-rutinoside (m/z 595). Anthocyanins exhibited a bathochromic shiftwith increasing pH and greater stability under acidic, refrigerated storage than at room temperature. Lipsticks formulated with 0-25% anthocyanins met SNI standards for pH and melting point. Antioxidant activity (IC50) improved with anthocyanins concentration, ranging from 24.52 ppm (0%) to 18.20 ppm (25%), compared to 12.91 ppm for Trolox and 15.02 ppm for RBFE. Higher anthocyanin concentration also produced more intense red coloration, demonstrating Binahong fruit's potential as a functional, health-conscious colorant for cosmetics formulations.