Abstract
Flavan-3-ol is a secondary metabolite in tea plant that is used as anti obesity agent. The diffi culty in obtaining Flavan-3-ol outof tea plant is due to dependency on season, need for large land, need for very intensive care and relatively low production. Therefore fl avan 3-ol production needs tobe developed in vitro culture technique. This technique can cope with the handicapes above. It caneffectively control production and requires less space. The purpose of this research was to enhance fl avan 3-ol production by modifyingmedium and precursor appropriately. The steps of this process were: (1) Callus induction through cultivating the tea shoot explants onmedium fi lled with some growth hormone, (2) fl avan-3-ol induction on callus culture using Cu 2+ elicitor. (3) Observation over callusgrowth, (4) Observation over qualitative characterizes of fl avan 3-ol. The result of the research using Cu 2+ elicitor shows that theproduction of fl avan-3-ol increases by 12.5%.