Abstract
This research aimed to know the bioactivity of polysaccharide krestin (PSK) with a different timing on formation of mice antibodyexposed by Mycobacterium tuberculosis. Mycobacterium tuberculosis was used as infection agent that had an abilitiy to be imunogenicand to lead immune system responsses decreased. Polysaccharides krestin was used as immunopotentiator which had a role to activatemacrophage and stimulate B cell in order to produce antibody. This research involved 30 adult female mice of Mus musculus strain Balb/C in the age of 8 €“10 weeks and in the weight between of 30 €“40 g. Mice were divided into six groups, as follows: group K asa control, was added only aquades; group K+ as positive control, was added only PSK; group K- as negative control, was exposedby M. tuberculosis only; group P1 was added PSK before being exposed by M. tuberculosis; group P2 was added PSK after beingexposed by M. tuberculosis; group P3 was added PSK before and after being exposed by M. tuberculosis. Polysaccharides krestin wasadded by gavage with single dose of 500 μg and M. tuberculosis was exposed intraperitoneally with concentration of 5x108 bacteryper ml. The bioactivity of PSK was observed by formation antibody using indirect ELISA test. The results of research were the averageof OD value of K group was 0,590 ± 0,042, K+ group was 0.641 ± 0.025, K- group was 1.044 ± 0.054, P1 group was 1.032 ± 0.125,P2 group was 1.127 ± 0.042, P3 group was 1.230 ± 0.097. The conclusion of this research showed that the adding of PSK increasedmice formation antibody. The time of adding before and after being exposed M. tuberculosis was the most potential to raise antibodyproduction on mice.