Abstract
During the study there was evaluated the effectiveness of treatment of acute catarrhal mastitis in cows using complex approach that included the application of antimicrobial preparation and testicular hyaluronidase. For the experiment, two groups of animals were formed, 10 heads each, with clinical signs of acute catarrhal inflammation of one of the udder quarters. All cows were given the drug “Vaccamast” in a dose of 10 ml intracisternally, as well as a supramuscular novocaine blockade on the day of diagnosis. In the experimental group, 1280 units of testicular hyaluronidase solution were additionally administered intracisternally. The results of the study showed that the relief of the inflammatory process was accompanied by an increase in the level of milk productivity: in the experimental group – by 6.2 liters (P< 0.05), in the control group – by 6.4 liters (P<0.01). After completing the therapeutic course, in the group where testicular hyaluronidase was used, the average concentration of somatic cells was 234.2 thousand/ml lower (P<0.05) compared with the control group, and the number of cows with a concentration of somatic cells of more than 500 thousand/ml was less than four times. In animals that were not injected with the enzyme, the average electrical conductivity of milk was 0.6 mSm/cm higher than in the group receiving testicular hyaluronidase (P<0.001). In addition, the number of individuals with electrical conductivity exceeding 7.0 ms/cm in the control group was twice as high. The duration of therapy of animals with the use of testicular hyaluronidase was 4.1 days, and without its use – 4.4 days. During a month of observation, no recurrence of mastitis was detected in the experimental group, whereas in two cows that did not receive the enzymatic preparation, mammary gland inflammation was rediagnosed. After the treatment, the activity of AsAT in the experimental group was 28.9 % lower (P<0.01) compared with the control group, and the de Ritis coefficient was 1.46 % lower (P<0.01). The activity of GGT using the enzyme decreased by 23.8 %, which led to a decrease in this indicator relative to the control by 41.9 % (P<0.01). Thus, the results of the study indicate that the intracistern application of testicular hyaluronidase increases the effectiveness of complex therapy of acute mastitis in cows during lactation.